Transcript: Human XM_017004438.1

PREDICTED: Homo sapiens STE20 related adaptor beta (STRADB), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
STRADB (55437)
Length:
2128
CDS:
615..1457

Additional Resources:

NCBI RefSeq record:
XM_017004438.1
NBCI Gene record:
STRADB (55437)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017004438.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195094 CTAATCTAGCTAGTGTCATTT pLKO.1 1670 3UTR 100% 13.200 18.480 N STRADB n/a
2 TRCN0000007074 GCCGTGATTCTATCCCACTTT pLKO.1 519 5UTR 100% 4.950 6.930 N STRADB n/a
3 TRCN0000418437 AGGAACACTGGTAACTATAAA pLKO_005 446 5UTR 100% 15.000 10.500 N STRADB n/a
4 TRCN0000007072 GCACTGGAGAATCTATTATTT pLKO.1 1567 3UTR 100% 15.000 10.500 N STRADB n/a
5 TRCN0000196884 GAAACCAGTATCCATCAATAC pLKO.1 370 5UTR 100% 10.800 7.560 N STRADB n/a
6 TRCN0000194701 CAGAGTACTATGACAAGGAAA pLKO.1 1638 3UTR 100% 4.950 3.465 N STRADB n/a
7 TRCN0000007076 GCTTTGGGTTATTTCTCCATT pLKO.1 593 5UTR 100% 4.950 3.465 N STRADB n/a
8 TRCN0000007075 CCTCCTTATAGCCCATTGGAT pLKO.1 1050 CDS 100% 3.000 2.100 N STRADB n/a
9 TRCN0000196530 GAACACAAGTTGAATCACTCA pLKO.1 332 5UTR 100% 2.640 1.848 N STRADB n/a
10 TRCN0000007073 CCAGTGGAACTCACACAGTAA pLKO.1 1156 CDS 100% 4.950 2.970 N STRADB n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017004438.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15321 pDONR223 100% 67.3% 8.1% None (many diffs) n/a
2 TRCN0000466482 CTGGCTGCCAGCGCATTTACCTTT pLX_317 20.8% 67.3% 8.1% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_08534 pDONR223 100% 66.9% 66.9% None 0_1ins414;195G>T n/a
4 ccsbBroad304_08534 pLX_304 0% 66.9% 66.9% V5 0_1ins414;195G>T n/a
5 TRCN0000480011 CGACCGGTTCTTATGCTTCATGCA pLX_317 32.2% 66.9% 66.9% V5 0_1ins414;195G>T n/a
6 TRCN0000488001 GGGCTCAGCGAAGGCACAAATGGC pLX_317 25.8% 66.9% 66.9% V5 (not translated due to prior stop codon) 0_1ins414;342C>T n/a
7 TRCN0000488545 CTCATACCTGATAGCGCAGGAGCC pLX_317 25.7% 66.8% 66.8% V5 0_1ins414;342C>T;840_841insG n/a
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