Construct: ORF TRCN0000480018
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013497.1_s317c1
- Derived from:
- ccsbBroadEn_10188
- DNA Barcode:
- TCTCTTGGGAGGTGCATTCGATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPIAL4G (644591)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480018
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 653598 | PPIAL4C | peptidylprolyl isomerase A ... | NM_001135789.3 | 100% | 100% | |
| 2 | human | 644591 | PPIAL4G | peptidylprolyl isomerase A ... | NM_001123068.1 | 99.3% | 98.1% | 16A>G;71A>T;206A>G |
| 3 | human | 105371242 | PPIAL4H | peptidylprolyl isomerase A ... | NM_001368128.1 | 99.1% | 98.1% | (many diffs) |
| 4 | human | 653505 | PPIAL4A | peptidylprolyl isomerase A ... | NM_001143883.3 | 98.7% | 98.1% | (many diffs) |
| 5 | human | 730262 | PPIAL4E | peptidylprolyl isomerase A ... | NM_001144032.2 | 98.5% | 98.1% | (many diffs) |
| 6 | human | 728945 | PPIAL4F | peptidylprolyl isomerase A ... | NM_001164262.2 | 98.5% | 98.1% | (many diffs) |
| 7 | human | 645142 | PPIAL4D | peptidylprolyl isomerase A ... | NM_001164261.1 | 98.3% | 97.5% | (many diffs) |
| 8 | human | 5478 | PPIA | peptidylprolyl isomerase A | NM_021130.5 | 91.9% | 86% | (many diffs) |
| 9 | human | 729739 | PPIAP46 | peptidylprolyl isomerase A ... | NR_045207.1 | 90.2% | (many diffs) | |
| 10 | human | 5478 | PPIA | peptidylprolyl isomerase A | XM_024446808.1 | 79.8% | 72.5% | (many diffs) |
| 11 | human | 100192204 | PPIAP30 | peptidylprolyl isomerase A ... | NR_036506.1 | 68.3% | (many diffs) | |
| 12 | human | 5478 | PPIA | peptidylprolyl isomerase A | NM_001300981.2 | 59.1% | 55.1% | (many diffs) |
| 13 | human | 5478 | PPIA | peptidylprolyl isomerase A | XM_024446809.1 | 59.1% | 55.1% | (many diffs) |
| 14 | human | 5478 | PPIA | peptidylprolyl isomerase A | XR_002956460.1 | 26.7% | (many diffs) | |
| 15 | human | 284996 | RNF149 | ring finger protein 149 | XR_001738713.2 | 15.7% | (many diffs) | |
| 16 | mouse | 268373 | Ppia | peptidylprolyl isomerase A | NM_008907.1 | 88.8% | 84.1% | (many diffs) |
| 17 | mouse | 668548 | Gm9234 | predicted pseudogene 9234 | XR_869917.3 | 58% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 561
- ORF length:
- 492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtcaactcc gtcgtctttt ttgacatcac cgtcgacggc aagcccttgg 121 gccgcatctc catcaaactg tttGCAGACA AGATTCCAAA GACAGCAGAA AACTTTCGTG 181 CTCTGAGCAC TGGAGAGAAA GGATTTCGTT ATAAGGGTTC CTGCTTTCAC AGAATTATTC 241 CAGGGTTTAT GTGTCAGGGT GGTGACTTCA CACGCCCTAA TGGCACCGGT GACAAGTCCA 301 TCTATGGGGA GAAATTTGAT GATGAGAACC TCATCCGAAA GCATACAGGT TCTGGCATCT 361 TGTCCATGGC AAATGCTGGA CCCAACACAA ATGGTTCCCA GTTTTTCATC TGCACTGCCA 421 AGACTGAGTG GTTGGATGGC AAGCATGTGG CCTTTGGCAA GGTGAAAGAA CGTGTGAATA 481 TTGTGGAAGC CATGGAGCAC TTTGGGTACA GGAATAGCAA GACCAGCAAG AAGATCACCA 541 TTGCTGACTG TGGACAATTC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 601 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 661 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCTC TTGGGAGGTG 721 CATTCGATCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt