Construct: ORF TRCN0000480052
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001070.1_s317c1
- Derived from:
- ccsbBroadEn_06886
- DNA Barcode:
- AGTCATCTCCAACCCCTGGCACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL36A (6173)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480052
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6173 | RPL36A | ribosomal protein L36a | NM_021029.6 | 100% | 100% | |
| 2 | human | 6166 | RPL36AL | ribosomal protein L36a like | NM_001001.5 | 89.9% | 99% | (many diffs) |
| 3 | human | 100529097 | RPL36A-HNRNPH2 | RPL36A-HNRNPH2 readthrough | NM_001199973.2 | 87.8% | 84.1% | (many diffs) |
| 4 | human | 100529097 | RPL36A-HNRNPH2 | RPL36A-HNRNPH2 readthrough | NM_001199974.2 | 66.2% | 60.3% | (many diffs) |
| 5 | mouse | 19982 | Rpl36a | ribosomal protein L36A | NM_019865.5 | 92.7% | 100% | (many diffs) |
| 6 | mouse | 66483 | Rpl36al | ribosomal protein L36A-like | NM_025589.4 | 87.7% | 100% | (many diffs) |
| 7 | mouse | 624713 | Gm6525 | predicted pseudogene 6525; ... | NR_036654.1 | 51% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 411
- ORF length:
- 318
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA TAAGTTGGCA CCATGGTTAA CGTCCCTAAA ACCCGCCGGA 121 CTTTCTGTAA GAAGTGTGGC AAGCACCAAC CCCATAAAGT GACACAGTAC AAGAAGGGCA 181 AGGATTCTCT GTACGCCCAG GGAAAGCGGC GTTATGACAG GAAGCAGAGT GGCTATGGTG 241 GGCAAACTAA GCCGATTTTC CGGAAAAAGG CTAAAACTAC AAAGAAGATT GTGCTAAGGC 301 TTGAGTGCGT TGAGCCCAAC TGCAGATCTA AGAGAATGCT GGCTATTAAA AGATGCAAGC 361 ATTTTGAACT GGGAGGAGAT AAGAAGAGAA AGGGCCAAGT GATCCAGTTC TTGCCAACTT 421 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 481 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 541 CTTGTGGAAA GGACGAAGTC ATCTCCAACC CCTGGCACAC ACGCGTTAAG TCgacaatca 601 acctctggat tacaaaattt gtgaaagatt