Construct: ORF TRCN0000480073
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010025.1_s317c1
- Derived from:
- ccsbBroadEn_06080
- DNA Barcode:
- GGACCCGAACGTCACTGCTATACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAD1 (1603)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480073
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1603 | DAD1 | defender against cell death 1 | NM_001344.4 | 99.7% | 99.1% | 59C>T |
| 2 | mouse | 13135 | Dad1 | defender against cell death 1 | NM_001113358.1 | 91.1% | 99.1% | (many diffs) |
| 3 | mouse | 13135 | Dad1 | defender against cell death 1 | NM_010015.4 | 91.1% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 429
- ORF length:
- 339
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCGGCGTC GGTAGTGTCT GTCATTTCGC 121 GGTTCTTAGA AGAGTACTTG AGCTCCATTC CGCAGCGTCT GAAGTTGCTG GACGCGTACC 181 TGCTGTATAT ACTGCTGACC GGGGCGCTGC AGTTCGGTTA CTGTCTCCTC GTGGGGACCT 241 TCCCCTTCAA CTCTTTTCTC TCGGGCTTCA TCTCTTGTGT GGGGAGTTTC ATCCTAGCGG 301 TTTGCCTGAG AATACAGATC AACCCACAGA ACAAAGCGGA TTTCCAAGGC ATCTCCCCAG 361 AGCGAGCCTT TGCTGATTTT CTCTTTGCCA GCACCATCCT GCACCTTGTT GTCATGAACT 421 TTGTTGGCTG CCTAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 481 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 541 TTTCTTGGCT TATATATCTT GTGGAAAGGA CGAGGACCCG AACGTCACTG CTATACCACG 601 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt