Construct: ORF TRCN0000480083
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004174.1_s317c1
- Derived from:
- ccsbBroadEn_04198
- DNA Barcode:
- GCGGAGGCGCGCTACTGTGAGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C16orf70 (80262)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480083
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80262 | C16orf70 | chromosome 16 open reading ... | NM_001320540.2 | 100% | 100% | |
| 2 | human | 80262 | C16orf70 | chromosome 16 open reading ... | NM_001320541.2 | 100% | 100% | |
| 3 | human | 80262 | C16orf70 | chromosome 16 open reading ... | NM_001320542.2 | 100% | 100% | |
| 4 | human | 80262 | C16orf70 | chromosome 16 open reading ... | NM_025187.5 | 100% | 100% | |
| 5 | human | 80262 | C16orf70 | chromosome 16 open reading ... | NM_001320543.2 | 93.3% | 93.3% | 145_146ins84 |
| 6 | human | 80262 | C16orf70 | chromosome 16 open reading ... | XM_006721284.3 | 81.3% | 81.3% | 354_644del |
| 7 | human | 80262 | C16orf70 | chromosome 16 open reading ... | XM_017023729.1 | 81.3% | 81.3% | 354_644del |
| 8 | human | 80262 | C16orf70 | chromosome 16 open reading ... | XM_011523350.3 | 79.3% | 78% | (many diffs) |
| 9 | human | 80262 | C16orf70 | chromosome 16 open reading ... | XM_017023731.1 | 58.1% | 55.3% | (many diffs) |
| 10 | human | 80262 | C16orf70 | chromosome 16 open reading ... | XM_017023730.1 | 47.3% | 45% | (many diffs) |
| 11 | mouse | 234678 | D230025D16Rik | RIKEN cDNA D230025D16 gene | NM_145604.2 | 92% | 96.9% | (many diffs) |
| 12 | mouse | 234678 | D230025D16Rik | RIKEN cDNA D230025D16 gene | XM_006530887.2 | 85.9% | 90.4% | (many diffs) |
| 13 | mouse | 234678 | D230025D16Rik | RIKEN cDNA D230025D16 gene | XM_006530889.2 | 51.7% | 54.7% | (many diffs) |
| 14 | mouse | 234678 | D230025D16Rik | RIKEN cDNA D230025D16 gene | XM_006530888.2 | 48.3% | 51.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1332
- ORF length:
- 1266
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggacctggag gtagtgcccg aacgctctct ggggaacgag caatgggaat 121 tcacgctggg aatgcctctg gctcaggcag tagccattct tcagaagcac tgtcgcatca 181 tcaaaaacgt ccaggttctc tacagtgaac agtctcctct aagccatgac ctcattctta 241 acctgactca ggacgggatc aaactaatgt ttgatgcttt caatcagaga cttaaggtga 301 tcgaagtatg tgatttgact aaagtaaagt taaaatattg tggcgtgcat tttaattctc 361 aggccatagc tcctaccatt gaacagattg accagtcttt tggcgcaacc catcctggag 421 tgtacaactc cgctgagcag ctcttccatc tcaacttcag aggactgtct ttctcttttc 481 agttagactc atggactgag gctccaaagt atgagcccaa ttttgcccat ggcctggctt 541 ctctccagat accccatgga gcaactgtaa aacgaatgta catctacagt ggcaacagcc 601 tgcaggatac caaggctccc atgatgcctc tgagctgttt cctgggcaat gtctatgctg 661 agagtgtaga tgttcttcga gatggaactg gacctgcagg tttacgactt cgcctacttg 721 ctgcaggttg tggacctggc ctattagcag atgccaagat gcgggtattt gaacgttcag 781 tgtattttgg tgattcctgc caagatgttc ttagcatgct tggctctcca cacaaagtct 841 tctataaatc agaagacaag atgaaaattc attctccttc ccctcataaa caagttccat 901 cgaagtgtaa tgactacttt tttaactact tcactcttgg agtggacatc ctctttgatg 961 cgaatacaca caaagtgaag aagtttgttc TACACACCAA TTACCCTGGG CATTATAATT 1021 TCAACATTTA TCATCGCTGT GAGTTCAAGA TCCCACTAGC CATAAAGAAA GAAAACGCAG 1081 ATGGTCAGAC AGAAACATGC ACGACCTACA GCAAGTGGGA CAACATCCAG GAGCTCCTGG 1141 GCCACCCTGT GGAGAAGCCT GTTGTCCTGC ACAGGTCTTC CTCCCCAAAC AACACCAACC 1201 CATTTGGTTC CACATTCTGC TTTGGTCTTC AGCGAATGAT CTTTGAGGTC ATGCAGAACA 1261 ACCACATTGC CTCGGTGACC CTGTATGGCC CCCCCAGGCC TGGTAGCCAC CTGAGAACAG 1321 CGGAACTCCC CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1381 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1441 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCG GAGGCGCGCT ACTGTGAGAA 1501 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t