Construct: ORF TRCN0000480085
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001962.1_s317c1
- Derived from:
- ccsbBroadEn_09487
- DNA Barcode:
- TCTCTGAATCAGCCCCCAACATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- B4GALNT2 (124872)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480085
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124872 | B4GALNT2 | beta-1,4-N-acetyl-galactosa... | NM_153446.3 | 99.7% | 99.8% | (many diffs) |
| 2 | human | 124872 | B4GALNT2 | beta-1,4-N-acetyl-galactosa... | NM_001159387.2 | 88.8% | 88.6% | (many diffs) |
| 3 | human | 124872 | B4GALNT2 | beta-1,4-N-acetyl-galactosa... | NM_001159388.1 | 84.6% | 84.8% | 0_1ins258;318C>T;843T>C |
| 4 | human | 124872 | B4GALNT2 | beta-1,4-N-acetyl-galactosa... | XM_017024173.1 | 84.6% | 84.8% | 0_1ins258;318C>T;843T>C |
| 5 | human | 124872 | B4GALNT2 | beta-1,4-N-acetyl-galactosa... | XM_011524314.2 | 56.1% | 55.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1767
- ORF length:
- 1698
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggagcgct ggcttttccg tgggaaaatt ccacgtggag gtggcctctc 121 gcggccggga atgtgtctcg gggacgcccg agtgtgggaa tcggctcggg agtgcgggct 181 tcggggatct ctgcttggaa ctcagaggcg ctgacccagc ctggggcccg tttgctgccc 241 acgggaggag ccgccgtcag ggctcgagat ttctgtggct cctcaagata ttggtcataa 301 tcctggtact tggcattgtt ggatttatgt tcggaagcat gttccttcaa gcagtgttca 361 gcagccccaa gccagaactc ccaagtcctg ccccgggtgt ccagaagctg aagcttctgc 421 ctgaggaacg tctcaggaac ctcttttcct acgatggaat ctggctgttc ccgaaaaatc 481 agtgcaaatg tgaagccaac aaagagcagg gaggttacaa ctttcaggat gcctatggcc 541 agagcgacct cccagcggtg aaagcgagga gacaggctga atttgaacac tttcagagga 601 gagaagggct gccccgccca ctgcccctgc tggtccagcc caatctcccc tttgggtacc 661 cagtccacgg agtggaggtg atgcccctgc acacggttcc catcccaggc ctccagtttg 721 aaggacccga tgcccccgtc tatgaggtca ccctgacagc ttctctgggg acactgaaca 781 cccttgctga tgtcccagac agtgtggtgc agggcagagg ccagaagcag ctgatcattt 841 ctaccagtga ccggaagctg ttgaagttca ttcttcagca cgtgacatac accagcacgg 901 ggtaccagca ccagaaggta gacatagtga gtctggagtc caggtcctca gtggccaagt 961 ttccagtgac catccgccat cctgtcatac ccaagctata cgaccctgga ccagagagga 1021 agctcagaaa cctggttacc attgctacca agactttcct ccgcccccac aagctcatga 1081 tcatgctccg gagtattcga gagtattacc cagacttgac cgtaatagtg gctgatgaca 1141 gccagaagcc cctggaaatt aaagacaacc acgtggagta ttacactatg ccctttggga 1201 agggttggtt tgctggtagg aacctggcca tatctcaggt caccaccaaa tacgttctct 1261 gggtggacga tgattttctc ttcaacgagg agaccaagat tgaggtgctg gtggatgtcc 1321 tggagaaaac agaactggac gtggtaggcg gcagtgtgct gggaaatgtg ttccagtttA 1381 AGTTGTTGCT GGAACAGAGT GAGAATGGGG CCTGCCTTCA CAAGAGGATG GGATTTTTCC 1441 AACCCCTGGA TGGCTTCCCC AGCTGCGTGG TGACCAGTGG CGTGGTCAAC TTCTTCCTGG 1501 CCCACACGGA GCGACTCCAA AGAGTTGGCT TTGATCCCCG CCTGCAACGA GTGGCTCACT 1561 CAGAATTCTT CATTGATGGG CTAGGGACCC TACTCGTGGG GTCATGCCCA GAAGTGATTA 1621 TAGGTCACCA GTCTCGGTCT CCAGTGGTGG ACTCAGAACT GGCTGCCCTA GAGAAGACCT 1681 ACAATACATA CCGGTCCAAC ACCCTCACCC GGGTCCAGTT CAAGCTGGCC CTCCACTACT 1741 TCAAGAACCA TCTCCAATGT GCCGCATTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1801 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1861 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTCTGAA 1921 TCAGCCCCCA ACATTAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1981 aagatt