Construct: ORF TRCN0000480089
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000499.1_s317c1
- Derived from:
- ccsbBroadEn_11051
- DNA Barcode:
- TCTGATCCGACTCTATAAGCTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP2R2C (5522)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480089
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5522 | PPP2R2C | protein phosphatase 2 regul... | NM_001206996.1 | 99.9% | 100% | 1236C>T |
| 2 | human | 5522 | PPP2R2C | protein phosphatase 2 regul... | NM_001363388.2 | 97.4% | 97.7% | (many diffs) |
| 3 | human | 5522 | PPP2R2C | protein phosphatase 2 regul... | NM_001206994.2 | 97.1% | 97% | (many diffs) |
| 4 | human | 5522 | PPP2R2C | protein phosphatase 2 regul... | NM_001206995.2 | 97.1% | 97% | (many diffs) |
| 5 | human | 5522 | PPP2R2C | protein phosphatase 2 regul... | NM_020416.4 | 95.8% | 95.7% | (many diffs) |
| 6 | human | 5522 | PPP2R2C | protein phosphatase 2 regul... | NM_181876.3 | 95.6% | 94.8% | (many diffs) |
| 7 | human | 5522 | PPP2R2C | protein phosphatase 2 regul... | XM_011513496.1 | 67.8% | 67.9% | 0_1ins414;822C>T |
| 8 | human | 5522 | PPP2R2C | protein phosphatase 2 regul... | XM_005247979.4 | 53.4% | 53.4% | 0_1ins600;636C>T |
| 9 | mouse | 269643 | Ppp2r2c | protein phosphatase 2, regu... | XM_006503972.3 | 89% | 97% | (many diffs) |
| 10 | mouse | 269643 | Ppp2r2c | protein phosphatase 2, regu... | NM_172994.2 | 86.6% | 95% | (many diffs) |
| 11 | mouse | 269643 | Ppp2r2c | protein phosphatase 2, regu... | XM_006503971.2 | 78.2% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1356
- ORF length:
- 1290
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aaagagagac acggctgaca tcatctctac cgttgagttc aaccacacgg 121 gagagctgct ggccacaggt gacaagggcg gccgggtcgt catcttccag cgggaaccag 181 agagtaaaaa tgcgccccac agccagggcg aatacgacgt gtacagcact ttccagagcc 241 acgagccgga gtttgactat ctcaagagcc tggagataga ggagaagatc aacaagatca 301 agtggctccc acagcagaac gccgcccact cactcctgtc caccaacgat aaaactatca 361 aattatggaa gattaccgaa cgagataaaa ggcccgaagg atacaacctg aaggatgaag 421 aggggaaact taaggacctg tccacggtga cgtcactgca ggtgccagtg ctgaagccca 481 tggatctgat ggtggaggtg agccctcgga ggatctttgc caatggccac acctaccaca 541 tcaactccat ctccgtcaac agtgactgcg agacctacat gtcggcggat gacctgcgca 601 tcaacctctg gcacctggcc atcaccgaca ggagcttcaa catcgtggac atcaagccgg 661 ccaacatgga ggaccttacg gaggtgatca cagcatctga gttccatccg caccactgca 721 acctcttcgt ctacagcagc agcaagggct ccctgcggct ctgcgacatg cgggcagctg 781 ccctgtgtga caagcattcc aagctctttg aagagcctga ggaccccagt aaccgctcat 841 tcttctcgga aatcatctcc tccgtgtccg acgtgaagtt cagccacagc ggccgctaca 901 tgctcacccg ggactacctt acagtcaagg tctgggacct gaacatggag gcaagaccca 961 tagagaccTA CCAGGTCCAT GACTACCTTC GGAGCAAGCT CTGTTCCCTG TACGAGAACG 1021 ACTGCATTTT CGACAAGTTT GAATGTGCCT GGAACGGGAG CGACAGCGTC ATCATGACCG 1081 GGGCCTACAA CAACTTCTTC CGCATGTTCG ATCGGAACAC CAAGCGGGAC GTGACCCTGG 1141 AGGCCTCGAG GGAAAGCAGC AAGCCCCGGG CTGTGCTCAA GCCACGGCGC GTGTGCGTGG 1201 GGGGCAAGCG CCGGCGTGAT GACATCAGTG TGGACAGCTT GGACTTCACC AAGAAGATCC 1261 TGCACACGGC CTGGCACCCG GCTGAGAACA TCATTGCCAT TGCCGCCACC AACAACCTGT 1321 ACATCTTCCA GGACAAGGTA AACTCTGACA TGCACTACCC AACTTTCTTG TACAAAGTGG 1381 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1441 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1501 ATCTGATCCG ACTCTATAAG CTTCAACGCG TTAAGTCgac aatcaacctc tggattacaa 1561 aatttgtgaa agatt