Construct: ORF TRCN0000480108
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003035.1_s317c1
- Derived from:
- ccsbBroadEn_14305
- DNA Barcode:
- CCACAAAGTCTCGAGAAGAACCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLR1B (84172)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480108
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84172 | POLR1B | RNA polymerase I subunit B | NM_001282774.2 | 99.8% | 99.6% | (many diffs) |
| 2 | human | 84172 | POLR1B | RNA polymerase I subunit B | NM_019014.6 | 83.7% | 82.9% | (many diffs) |
| 3 | human | 84172 | POLR1B | RNA polymerase I subunit B | NM_001282772.2 | 81% | 80.2% | (many diffs) |
| 4 | human | 84172 | POLR1B | RNA polymerase I subunit B | NM_001371969.1 | 80.4% | 79.6% | (many diffs) |
| 5 | human | 84172 | POLR1B | RNA polymerase I subunit B | NM_001137604.3 | 78.8% | 77.9% | (many diffs) |
| 6 | human | 84172 | POLR1B | RNA polymerase I subunit B | NM_001282777.2 | 71.5% | 70.7% | (many diffs) |
| 7 | human | 84172 | POLR1B | RNA polymerase I subunit B | NM_001282779.2 | 71.5% | 70.7% | (many diffs) |
| 8 | human | 84172 | POLR1B | RNA polymerase I subunit B | NM_001371970.1 | 71.5% | 70.7% | (many diffs) |
| 9 | human | 84172 | POLR1B | RNA polymerase I subunit B | NM_001282776.2 | 65.1% | 64.4% | (many diffs) |
| 10 | human | 84172 | POLR1B | RNA polymerase I subunit B | NM_001371971.1 | 65.1% | 64.4% | (many diffs) |
| 11 | mouse | 20017 | Polr1b | polymerase (RNA) I polypept... | XM_006499025.3 | 74.4% | 77.7% | (many diffs) |
| 12 | mouse | 20017 | Polr1b | polymerase (RNA) I polypept... | NM_009086.2 | 71.3% | 76.2% | (many diffs) |
| 13 | mouse | 20017 | Polr1b | polymerase (RNA) I polypept... | XM_006499026.2 | 51.1% | 55.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2925
- ORF length:
- 2856
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatcctggc atccggtggc ggaacctgcc cagcgggcct agcctaaagc 121 acttgactga cccctcttat ggaatcccgc gggaacagca aaaggcagcg ttgcaggagc 181 tgacgcgggc gcacgtggag tccttcaact acgctgtgca cgagggtctc ggcctcgcgg 241 tgcaggctat acctcccttt gaatttgctt tcaaagatga gcgtatctct tttactattc 301 tggatgctgt tatcagtcca cctacagttc caaaagggac catctgcaaa gaggccaatg 361 tttatccagc agaatgccgg ggccgaagga gtacctaccg tgggaagttg acagctgata 421 tcaactgggc agtgaatgga atctcaaaag gaatcattaa gcagtttctt ggctatgttc 481 ccatcatggt gaaatccaag ctttgcaact tacgtaacct tcccccacaa gccctcattg 541 agcaccatga ggaggcagag gaaatggggg gctattttat aatcaatggc attgaaaaag 601 tcatccgaat gttgattatg cctcggagaa attttcccat tgcaatgata agaccaaaat 661 ggaaaaccag agggcctggt tatactcagt atggagtttc aatgcactgt gtgagggaag 721 aacattccgc tgtcaatatg aacctccact acttggaaaa tggcacagtt atgttgaact 781 ttatttaccg aaaagaactg ttctttcttc ctttgggatt tgcacttaag gcacttgtca 841 gcttttctga ttatcagatc tttcaggagc tcatcaaagg aaaagaggat gattctttcc 901 ttaggaactc tgtttctcag atgttaagga ttgtaatgga agagggttgt ttgacacaaa 961 aacaggtcct taactaccta ggtgaatgct tcagagtaaa actcaatgtt cctgactggt 1021 acccaaatga gcaagctgcg gagttcctgt ttaaccagtg catctgtatc cacttgaaat 1081 ccaatactga aaagttttat atgctttgtc tcatgacgcg aaagctcttt gctttagcca 1141 aaggagggtg catggaggac aatcctgata gtttggtgaa ccaggaagtc ctcacaccgg 1201 gtcagctctt ccttatgttc ctgaaggggt cactcccatt gatggagctc cccaccgatc 1261 atacagtgtt gagagagaaa agaattcctc cctggatgga agtggtcctt atacccatga 1321 caggaaaacc aagtctgtac ccaggattgt tcctttttac cactccttgt agactggtac 1381 ggcctgtgca gaacttagca ttgggcaaag aagagctaat tggaactatg gaacagatct 1441 tcatgaatgt cgctatcttt gaggatgaag tttttgctgg agttaccaca caccaggaac 1501 tctttccaca cagcctgctg agtgtgattg ccaacttcat ccctttctct gatcacaacc 1561 agagtccacg gaacatgtac caatgccaga tgggtaagca aactatgggc tttccacttc 1621 tcacttatca agaccgatcg gataacaaac tgtatcgtct tcagactcct cagagtccct 1681 tggtgagacc ctccatgtat gattattatg acatggataa ctatccaatt gggaccaatg 1741 ccatcgttgc tgtgatttct tacactggct atgatatgga agatgccatg attgtgaata 1801 aggcctcttg ggaacgaggc tttgcccatg gaagtgtcta caagtctgag ttcatagacc 1861 tctctgaaaa aattaaacaa ggagatagta gcctggtgtt tggcatcaaa cctggtgacc 1921 cacgcgttct gcagaagtta gatgacgatg gattgccgtt tataggagca aaactgcagt 1981 acggagatcc gtattacagc tacctcaacc tcaacaccgg ggaaagtttt gtgatgtact 2041 ataagagtaa agaaaattgt gttgtggata acatcaaagt gtgcagtaat gacactggga 2101 gtggaaaatt caagtgtgtt tgcatcacta tgagagtgcc tcggaaccca actatcggag 2161 ataaatttgc cagtcgccat gggcagaagg gcattttaag cagattgtgg ccggctgagg 2221 acatgccttt tactgagagt gggatggtcc cagacattct gttcaatccc catggttttc 2281 catcccgcat gaccattggg atgttaattg agagtatggc cgggaagtct gcagctttgc 2341 atggtctctg ccatgatgct acacccttca tcttctcaga ggagaactcg gccttagaat 2401 actttggtga gatgttaaag gctgctggct acaatttcta tggcaccgag aggttatata 2461 gtggcatcag tgggctagaa ctggaagcag acatcttcat aggagtggtt tattatcagc 2521 gcttacgcca tatggtctca gacaaatttc aagtaaggac aactggagcc cgagacagag 2581 tcaccaacca gcctattggg ggaagaaatg tccagggtgg aatccgtttt ggggagatgg 2641 aacgggatgc gcttttagct catggtacaT CTTTTCTCCT TCATGACCGC CTCTTCAACT 2701 GCTCAGATCG GTCGGTAGCC CATGTGTGTG TGAAGTGTGG CAGTTTACTC TCTCCACTGT 2761 TGGAGAAGCC ACCCCCTTCT TGGTCTGCCA TGCGCAACAG AAAATACAAC TGTACTCTGT 2821 GTAGTCGCAG TGACACTATC GATACTGTTT CTGTGCCTTA TGTTTTTCGG TATTTTGTAG 2881 CTGAACTGGC AGCTATGAAC ATCAAAGTGA AACTGGATGT TGTTTTGCCA ACTTTCTTGT 2941 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3001 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3061 GAAAGGACGA CCACAAAGTC TCGAGAAGAA CCGGACGCGT TAAGTCgaca atcaacctct 3121 ggattacaaa atttgtgaaa gatt