Construct: ORF TRCN0000480153
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009378.1_s317c1
- Derived from:
- ccsbBroadEn_09053
- DNA Barcode:
- ATAGCTACAGGCGAGTTACGCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUSP16 (80824)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480153
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80824 | DUSP16 | dual specificity phosphatas... | NM_030640.3 | 99.8% | 99.8% | 1419C>T;1511A>T |
| 2 | human | 80824 | DUSP16 | dual specificity phosphatas... | XM_006719155.2 | 99.8% | 99.8% | 1419C>T;1511A>T |
| 3 | human | 80824 | DUSP16 | dual specificity phosphatas... | XM_011520856.1 | 99.8% | 99.8% | 1419C>T;1511A>T |
| 4 | human | 80824 | DUSP16 | dual specificity phosphatas... | XM_005253488.3 | 77.6% | 72% | (many diffs) |
| 5 | human | 80824 | DUSP16 | dual specificity phosphatas... | XM_017019988.1 | 77.6% | 72% | (many diffs) |
| 6 | mouse | 70686 | Dusp16 | dual specificity phosphatas... | NM_130447.3 | 88% | 90.5% | (many diffs) |
| 7 | mouse | 70686 | Dusp16 | dual specificity phosphatas... | NM_001048054.2 | 47% | 49.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2064
- ORF length:
- 1995
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccatgag atgattggaa ctcaaattgt tactgagagg ttggtggctc 121 tgctggaaag tggaacggaa aaagtgctgc taattgatag ccggccattt gtggaataca 181 atacatccca cattttggaa gccattaata tcaactgctc caagcttatg aagcgaaggt 241 tgcaacagga caaagtgtta attacagagc tcatccagca ttcagcgaaa cataaggttg 301 acattgattg cagtcagaag gttgtagttt acgatcaaag ctcccaagat gttgcctctc 361 tctcttcaga ctgttttctc actgtacttc tgggtaaact ggagaagagc ttcaactctg 421 ttcacctgct tgcaggtggg tttgctgagt tctctcgttg tttccctggc ctctgtgaag 481 gaaaatccac tctagtccct acctgcattt ctcagccttg cttacctgtt gccaacattg 541 ggccaacccg aattcttccc aatctttatc ttggctgcca gcgagatgtc ctcaacaagg 601 agctgatgca gcagaatggg attggttatg tgttaaatgc cagcaatacc tgtccaaagc 661 ctgactttat ccccgagtct catttcctgc gtgtgcctgt gaatgacagc ttttgtgaga 721 aaattttgcc gtggttggac aaatcagtag atttcattga gaaagcaaaa gcctccaatg 781 gatgtgttct agtgcactgt ttagctggga tctcccgctc cgccaccatc gctatcgcct 841 acatcatgaa gaggatggac atgtctttag atgaagctta cagatttgtg aaagaaaaaa 901 gacctactat atctccaaac ttcaattttc tgggccaact cctggactat gagaagaaga 961 ttaagaacca gactggagca tcagggccaa agagcaaact caagctgctg cacctggaga 1021 agccaaatga acctgtccct gctgtctcag agggtggaca gaaaagcgag acgcccctca 1081 gtccaccctg tgccgactct gctacctcag aggcagcagg acaaaggccc gtgcatcccg 1141 ccagcgtgcc cagcgtgccc agcgtgcagc cgtcgctgtt agaggacagc ccgctggtac 1201 aggcgctcag tgggctgcac ctgtccgcag acaggctgga agacagcaat aagctcaagc 1261 gttccttctc tctggatatc aaatcagttt catattcagc cagcatggca gcatccttac 1321 atggcttctc ctcatcagaa gatgctttgg aatactacaa accttccact actctggatg 1381 ggaccaacaa gctatgccag ttctcccctg ttcaggaact atcggagcag actcccgaaa 1441 ccagtcctga taaggaggaa gccagcatcc ccaagaagct gcagactgcc aggccttcag 1501 acagccagag caagcgattg cattcggtca gaaccagcag cagtggcacc gcccagaggt 1561 cccttttatc tccactgctt cgaagtggga gcgtggagga caattaccac accagcttcc 1621 ttttcggcct ttccaccagc cagcagcacc tcacgaagtc tgctggcctg ggCCTTAAGG 1681 GCTGGCACTC GGATATCTTG GCCCCCCAGA CCTCTACCCC TTCCCTGACC AGCAGCTGGT 1741 ATTTTGCCAC AGAGTCCTCA CACTTCTACT CTGCCTCAGC CATCTACGGA GGCAGTGCCA 1801 GTTACTCTGC CTACAGCTGC AGCCAGCTGC CCACTTGCGG AGACCAAGTC TATTCTGTGC 1861 GCAGGCGGCA GAAGCCAAGT GACAGAGCTG ACTCGCGGCG GAGCTGGCAT GAAGAGAGCC 1921 CCTTTGAAAA GCAGTTTAAA CGCAGAAGCT GCCAAATGGA ATTTGGAGAG AGCATCATGT 1981 CAGAGAACAG GTCACGGGAA GAGCTGGGGA AAGTGGGCAG TCAGTCTAGC TTTTCGGGCA 2041 GCATGGAAAT CATTGAGGTC TCCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2101 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2161 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TAGCTACAGG 2221 CGAGTTACGC AGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2281 att