Transcript: Human XM_006719155.2

PREDICTED: Homo sapiens dual specificity phosphatase 16 (DUSP16), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DUSP16 (80824)
Length:
5102
CDS:
492..2489

Additional Resources:

NCBI RefSeq record:
XM_006719155.2
NBCI Gene record:
DUSP16 (80824)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006719155.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000052013 CCGGCCATTTGTGGAATACAA pLKO.1 584 CDS 100% 5.625 7.875 N DUSP16 n/a
2 TRCN0000052014 CCCGAATTCTTCCCAATCTTT pLKO.1 970 CDS 100% 0.000 0.000 N DUSP16 n/a
3 TRCN0000299954 CCCGAATTCTTCCCAATCTTT pLKO_005 970 CDS 100% 0.000 0.000 N DUSP16 n/a
4 TRCN0000303835 CATTTGAGAGATCAGCTAATA pLKO_005 2659 3UTR 100% 13.200 9.240 N DUSP16 n/a
5 TRCN0000052017 GCAACAGGACAAAGTGTTAAT pLKO.1 665 CDS 100% 13.200 9.240 N DUSP16 n/a
6 TRCN0000310527 GCAACAGGACAAAGTGTTAAT pLKO_005 665 CDS 100% 13.200 9.240 N DUSP16 n/a
7 TRCN0000379533 GATCTCAAATATTAGTCTTTG pLKO_005 2841 3UTR 100% 10.800 7.560 N DUSP16 n/a
8 TRCN0000052015 CATCAGAAGATGCTTTGGAAT pLKO.1 1756 CDS 100% 4.950 3.465 N DUSP16 n/a
9 TRCN0000299955 CATCAGAAGATGCTTTGGAAT pLKO_005 1756 CDS 100% 4.950 3.465 N DUSP16 n/a
10 TRCN0000052016 GCCTCCAATGGATGTGTTCTA pLKO.1 1194 CDS 100% 4.950 3.465 N DUSP16 n/a
11 TRCN0000299953 GCCTCCAATGGATGTGTTCTA pLKO_005 1194 CDS 100% 4.950 3.465 N DUSP16 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006719155.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09053 pDONR223 100% 99.8% 99.8% None 1419C>T;1511A>T n/a
2 ccsbBroad304_09053 pLX_304 0% 99.8% 99.8% V5 1419C>T;1511A>T n/a
3 TRCN0000480153 ATAGCTACAGGCGAGTTACGCAGT pLX_317 19.9% 99.8% 99.8% V5 1419C>T;1511A>T n/a
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