Construct: ORF TRCN0000480198
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002234.1_s317c1
- Derived from:
- ccsbBroadEn_12250
- DNA Barcode:
- CAGTAAACTCCATAGGACTTAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIMS2 (55679)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480198
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_001256542.1 | 21.6% | 21.6% | 1_444del |
| 2 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_024452985.1 | 21.6% | 21.6% | 1_444del |
| 3 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_024452986.1 | 21.6% | 21.6% | 1_444del |
| 4 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_006712627.4 | 18.2% | 18.2% | 1_552del |
| 5 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_017004469.1 | 17.2% | 17.2% | 1_591del |
| 6 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_005263710.2 | 15% | 15% | 1_693del |
| 7 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_011511453.1 | 12.6% | 12.6% | 1_849del |
| 8 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_001161404.1 | 12.2% | 12.2% | 1_885del |
| 9 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_024452983.1 | 12.2% | 12.2% | 1_885del |
| 10 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XM_024452984.1 | 12.2% | 12.2% | 1_885del |
| 11 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_001161403.2 | 12% | 12% | 1_900del |
| 12 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_001136037.3 | 11.2% | 11.2% | 1_966del |
| 13 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | NM_017980.4 | 11.2% | 11.2% | 1_972del |
| 14 | human | 55679 | LIMS2 | LIM zinc finger domain cont... | XR_922961.2 | 8.5% | 1_1064del;1165_1166ins22 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 320
- ORF end:
- 443
- ORF length:
- 123
- Sequence:
-
1 TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG 61 TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG 121 TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC 181 TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 241 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 301 TTTGTACAAA AAAGTTGGCA TGAAGCCCGT GTGTAAGAGG TGCTACGAGA AGTTCCCGCT 361 GGAGCTGAAG AAGCGGCTGA AGAAGCTGTC GGAGCTGACC TCCCGCAAGG CCCAGCCCAA 421 GGCCACAGAC CTCAACTCTG CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 481 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 541 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA GTAAACTCCA 601 TAGGACTTAA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 661 tt