Construct: ORF TRCN0000480213
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009528.1_s317c1
- Derived from:
- ccsbBroadEn_04763
- DNA Barcode:
- CGCTTCAGCCCCCTCAGAATTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRAPPC6B (122553)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480213
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 122553 | TRAPPC6B | trafficking protein particl... | NM_177452.4 | 100% | 100% | |
| 2 | human | 122553 | TRAPPC6B | trafficking protein particl... | NM_001079537.2 | 82.2% | 82.2% | 266_349del |
| 3 | human | 122553 | TRAPPC6B | trafficking protein particl... | XM_017020964.2 | 77.3% | 76.7% | (many diffs) |
| 4 | human | 122553 | TRAPPC6B | trafficking protein particl... | XM_011536409.2 | 58.2% | 58.2% | 0_1ins114;152_235del |
| 5 | mouse | 78232 | Trappc6b | trafficking protein particl... | NM_030057.3 | 76.1% | 81% | (many diffs) |
| 6 | mouse | 78232 | Trappc6b | trafficking protein particl... | NM_001306217.1 | 73.9% | 64.1% | (many diffs) |
| 7 | mouse | 78232 | Trappc6b | trafficking protein particl... | NM_001306218.1 | 54.2% | 58.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 480
- ORF length:
- 390
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCGGATGA GGCGTTGTTT TTGCTTCTCC 121 ATAACGAGAT GGTGTCTGGA GTGTACAAGT CCGCGGAGCA GGGGGAGGTG GAAAACGGAC 181 GATGTATTAC TAAGCTGGAA AACATGGGGT TTCGAGTGGG ACAAGGATTG ATAGAAAGGT 241 TTACAAAAGA TACTGCAAGG TTCAAGGATG AGTTAGATAT CATGAAGTTC ATTTGTAAAG 301 ATTTTTGGAC TACGGTATTC AAGAAACAAA TCGACAATCT AAGGACAAAT CATCAGTATT 361 TAGCATTTAC GTGTGGCTTA ATCAGAGGTG GCTTATCAAA CTTGGGAATA AAAAGTATTG 421 TAACAGCTGA AGTGTCTTCA ATGCCTGCTT GCAAATTTCA GGTGATGATA CAGAAGCTGT 481 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 541 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 601 TTTATATATC TTGTGGAAAG GACGACGCTT CAGCCCCCTC AGAATTATCA CGCGTTAAGT 661 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt