Construct: ORF TRCN0000480220
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007281.1_s317c1
- Derived from:
- ccsbBroadEn_01539
- DNA Barcode:
- ACGTAATAGCCACAGACAGCGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLAMF1 (6504)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480220
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6504 | SLAMF1 | signaling lymphocytic activ... | NM_003037.4 | 100% | 100% | |
| 2 | human | 6504 | SLAMF1 | signaling lymphocytic activ... | XM_017002130.2 | 94.4% | 92.1% | (many diffs) |
| 3 | human | 6504 | SLAMF1 | signaling lymphocytic activ... | XM_005245456.4 | 91% | 91% | 698_699ins90 |
| 4 | human | 6504 | SLAMF1 | signaling lymphocytic activ... | NM_001330754.1 | 90.8% | 73.3% | 790_872del;1071_1072insTGACACTTCCAGAGAGC |
| 5 | human | 6504 | SLAMF1 | signaling lymphocytic activ... | XM_011509905.3 | 58.5% | 58.2% | 0_1ins412;2_3insGAGCA |
| 6 | human | 6504 | SLAMF1 | signaling lymphocytic activ... | XM_017002131.2 | 52.4% | 36.8% | (many diffs) |
| 7 | human | 6504 | SLAMF1 | signaling lymphocytic activ... | NR_104399.3 | 24.8% | 1_99del;889_890ins74;1031_3675del | |
| 8 | human | 6504 | SLAMF1 | signaling lymphocytic activ... | NR_104401.3 | 24.6% | (many diffs) | |
| 9 | human | 6504 | SLAMF1 | signaling lymphocytic activ... | NR_104400.3 | 22.4% | 1_99del;799_800ins164;941_3585del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1074
- ORF length:
- 1005
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatcccaag gggctcctct ccttgacctt cgtgctgttt ctctccctgg 121 cttttggggc aagctacgga acaggtgggc gcatgatgaa ctgcccaaag attctccggc 181 agttgggaag caaagtgctg ctgcccctga catatgaaag gataaataag agcatgaaca 241 aaagcatcca cattgtcgtc acaatggcaa aatcactgga gaacagtgtc gagaacaaaa 301 tagtgtctct tgatccatcc gaagcaggcc ctccacgtta tctaggagat cgctacaagt 361 tttatctgga gaatctcacc ctggggatac gggaaagcag gaaggaggat gagggatggt 421 accttatgac cctggagaaa aatgtttcag ttcagcgctt ttgcctgcag ttgaggcttt 481 atgagcaggt ctccactcca gaaattaaag ttttaaacaa gacccaggag aacgggacct 541 gcaccttgat actgggctgc acagtggaga agggggacca tgtggcttac agctggagtg 601 aaaaggcggg cacccaccca ctgaacccag ccaacagctc ccacctcctg tccctcaccc 661 tcggccccca gcatgctgac aatatctaca tctgcaccgt gagcaaccct atcagcaaca 721 attcccagac cttcagcccg tggcccggat gcaggacaga cccctcagaa acaaaaccat 781 gggcagtgta tgcTGGGCTG TTAGGGGGTG TCATCATGAT TCTCATCATG GTGGTAATAC 841 TACAGTTGAG AAGAAGAGGT AAAACGAACC ATTACCAGAC AACAGTGGAA AAAAAAAGCC 901 TTACGATCTA TGCCCAAGTC CAGAAACCAG GTCCTCTTCA GAAGAAACTT GACTCCTTCC 961 CAGCTCAGGA CCCTTGCACC ACCATATATG TTGCTGCCAC AGAGCCTGTC CCAGAGTCTG 1021 TCCAGGAAAC AAATTCCATC ACAGTCTATG CTAGTGTGAC ACTTCCAGAG AGCTTGCCAA 1081 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1141 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1201 TATCTTGTGG AAAGGACGAA CGTAATAGCC ACAGACAGCG TCCACGCGTT AAGTCgacaa 1261 tcaacctctg gattacaaaa tttgtgaaag att