Construct: ORF TRCN0000480230
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018090.2_s317c1
- Derived from:
- ccsbBroadEn_00965
- DNA Barcode:
- TCTCGAACATTAGCTTCACAAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMAD9 (4093)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480230
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4093 | SMAD9 | SMAD family member 9 | NM_005905.6 | 100% | 100% | |
| 2 | human | 4093 | SMAD9 | SMAD family member 9 | NM_001127217.2 | 92% | 92% | 669_779del |
| 3 | human | 4093 | SMAD9 | SMAD family member 9 | XM_005266401.3 | 90.6% | 81.8% | (many diffs) |
| 4 | human | 4093 | SMAD9 | SMAD family member 9 | XM_006719827.3 | 83.8% | 75.8% | (many diffs) |
| 5 | human | 4093 | SMAD9 | SMAD family member 9 | XM_005266403.3 | 76.5% | 65.5% | (many diffs) |
| 6 | human | 4093 | SMAD9 | SMAD family member 9 | XM_005266404.3 | 71.6% | 63.6% | (many diffs) |
| 7 | mouse | 55994 | Smad9 | SMAD family member 9 | NM_019483.5 | 87.1% | 96.7% | (many diffs) |
| 8 | mouse | 55994 | Smad9 | SMAD family member 9 | XM_006501728.3 | 87.1% | 96.7% | (many diffs) |
| 9 | mouse | 55994 | Smad9 | SMAD family member 9 | XM_006501729.3 | 84.6% | 94.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1356
- ORF length:
- 1290
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ctccaccacc cccatcagct ccctcttctc cttcaccagc cccgcagtga 121 agagactgct aggctggaag caaggagatg aagaggaaaa gtgggcagag aaggcagtgg 181 actctctagt gaagaagtta aagaagaaga agggagccat ggacgagctg gagagggctc 241 tcagctgccc ggggcagccc agcaaatgcg tcacgattcc ccgctccctg gacgggcggc 301 tgcaggtgtc ccaccgcaag ggcctgcccc atgtgattta ctgtcgcgtg tggcgctggc 361 cggatctgca gtcccaccac gagctgaagc cgctggagtg ctgtgagttc ccatttggct 421 ccaagcagaa agaagtgtgc attaaccctt accactaccg ccgggtggag actccagtac 481 tgcctcctgt gctcgtgcca agacacagtg aatataaccc ccagctcagc ctcctggcca 541 agttccgcag cgcctccctg cacagtgagc cactcatgcc acacaacgcc acctatcctg 601 actctttcca gcagcctccg tgctctgcac tccctccctc acccagccac gcgttctccc 661 agtccccgtg cacggccagc taccctcact ccccaggaag tccttctgag ccagagagtc 721 cctatcaaca ctcagacttt cgaccagttt gttacgagga gccccagcac tggtgctcgg 781 tcgcctacta tgaactgaac aaccgagttg gggagacatt ccaggcttcc tcccgaagtg 841 tgctcataga tgggttcacc gacccttcaa ataacaggaa cagattctgt cttggacttc 901 tttctaatgt aaacagaaac tcaacgatag aaaataccag gagacatata ggaaagggtg 961 tgcacttgta cTACGTCGGG GGAGAGGTGT ATGCCGAGTG CGTGAGTGAC AGCAGCATCT 1021 TTGTGCAGAG CCGGAACTGC AACTATCAAC ACGGCTTCCA CCCAGCTACC GTCTGCAAGA 1081 TCCCCAGCGG CTGCAGCCTC AAGGTCTTCA ACAACCAGCT CTTCGCTCAG CTCCTGGCCC 1141 AGTCAGTTCA CCACGGCTTT GAAGTCGTGT ATGAACTGAC CAAGATGTGT ACTATCCGGA 1201 TGAGTTTTGT TAAGGGTTGG GGTGCTGAGT ATCATCGCCA GGATGTCACC AGCACCCCCT 1261 GCTGGATTGA GATTCATCTT CATGGGCCAC TGCAGTGGCT GGACAAAGTT CTGACTCAGA 1321 TGGGCTCTCC ACATAACCCC ATTTCTTCAG TGTCTTACCC AACTTTCTTG TACAAAGTGG 1381 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1441 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1501 ATCTCGAACA TTAGCTTCAC AAGTCACGCG TTAAGTCgac aatcaacctc tggattacaa 1561 aatttgtgaa agatt