Construct: ORF TRCN0000480238
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013981.3_s317c1
- Derived from:
- ccsbBroadEn_04606
- DNA Barcode:
- GGGTTTGGCTTAGTACCATGTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBCK (93627)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480238
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93627 | TBCK | TBC1 domain containing kinase | NM_033115.5 | 99.9% | 99.8% | 607C>G |
| 2 | human | 93627 | TBCK | TBC1 domain containing kinase | NM_001163437.3 | 94.6% | 88.3% | (many diffs) |
| 3 | human | 93627 | TBCK | TBC1 domain containing kinase | NM_001163435.3 | 92.9% | 92.7% | 266_454del;796C>G |
| 4 | human | 93627 | TBCK | TBC1 domain containing kinase | NM_001163436.4 | 92.9% | 92.7% | 266_454del;796C>G |
| 5 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_011532417.2 | 92.9% | 92.7% | 266_454del;796C>G |
| 6 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_017008846.1 | 92.9% | 92.7% | 266_454del;796C>G |
| 7 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_024454281.1 | 92.9% | 92.7% | 266_454del;796C>G |
| 8 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_024454282.1 | 92.9% | 92.7% | 266_454del;796C>G |
| 9 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_017008848.1 | 92.2% | 87.7% | (many diffs) |
| 10 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_017008847.2 | 88.8% | 88.6% | 266_454del;796C>G;2571_2572ins108 |
| 11 | human | 93627 | TBCK | TBC1 domain containing kinase | NM_001290768.2 | 86.8% | 86.7% | 0_1ins327;280C>G |
| 12 | human | 93627 | TBCK | TBC1 domain containing kinase | XM_017008849.1 | 86.8% | 86.7% | 0_1ins327;280C>G |
| 13 | human | 93627 | TBCK | TBC1 domain containing kinase | XR_001741354.2 | 72.6% | (many diffs) | |
| 14 | human | 93627 | TBCK | TBC1 domain containing kinase | XR_938800.3 | 71.3% | (many diffs) | |
| 15 | human | 93627 | TBCK | TBC1 domain containing kinase | XR_001741353.2 | 70% | (many diffs) | |
| 16 | human | 93627 | TBCK | TBC1 domain containing kinase | XR_002959772.1 | 28.6% | (many diffs) | |
| 17 | mouse | 271981 | Tbck | TBC1 domain containing kinase | NM_001163455.2 | 82.9% | 88.8% | (many diffs) |
| 18 | mouse | 271981 | Tbck | TBC1 domain containing kinase | XM_030252639.1 | 80.5% | 85.3% | (many diffs) |
| 19 | mouse | 271981 | Tbck | TBC1 domain containing kinase | XM_006501553.4 | 66.1% | 67.4% | (many diffs) |
| 20 | mouse | 271981 | Tbck | TBC1 domain containing kinase | XR_001783696.2 | 33.3% | (many diffs) | |
| 21 | mouse | 271981 | Tbck | TBC1 domain containing kinase | XR_003954329.1 | 31.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2556
- ORF length:
- 2490
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tcccctgaag gacgctgaaa tgggagcctt taccttcttt gcctcggctc 121 tgccacatga tgtttgtgga agcaatggac ttcctctcac accaaattcc atcaaaattt 181 tagggcgctt tcaaatcctt aaaaccatca cccatcccag actctgccag tatgtggata 241 tttctagggg aaagcatgaa cgactagtgg tcgtggctga acattgtgaa cgtagtctgg 301 aagacttgct tcgagaaagg aaacctgtga ggtatccctc gtacttggcc cctgaggtaa 361 ttgcacaggg aattttcaaa accactgatc acatgccaag taaaaaacca ttgccttctg 421 gccccaaatc agatgtatgg tctcttggaa tcattttatt tgagctttgt gtgggaagaa 481 aattatttca gagcttggat atttctgaaa gactaaaatt tttgcttact ttggattgtg 541 tagatgacac tttaatagtt ctggctgaag agcatggttg tttggacatt ataaaggagc 601 ttcctgaaac tgtgatagat cttttgaata agtgccttac cttccatcct tctaagaggc 661 caaccccaga tgaattaatg aaggacaaag tattcagtga ggtatcacct ttatataccc 721 cctttaccaa acctgccagt ctgttttcat cttctctgag atgtgctgat ttaactctgc 781 ctgaggatat cagtcagttg tgtaaagata taaataatga ttacctggca gaaagatcta 841 ttgaagaagt gtattacctt tggtgtttgg ctggaggtga cttggagaaa gagcttgtca 901 acaaggaaat cattcgatcc aaaccaccta tctgcacact ccccaatttt ctctttgagg 961 atggtgaaag ctttggacaa ggtcgagata gaagctcgct tttagatgat accactgtga 1021 cattgtcgtt atgccagcta agaaatagat tgaaagatgt tggtggagaa gcattttacc 1081 cattacttga agatgaccag tctaatttac ctcattcaaa cagcaataat gagttgtctg 1141 cagctgccac gctcccttta atcatcagag agaaggatac agagtaccaa ctaaatagaa 1201 ttattctctt cgacaggctg ctaaaggctt atccatataa aaaaaaccaa atctggaaag 1261 aagcaagagt tgacattcct cctcttatga gaggtttaac ctgggctgct cttctgggag 1321 ttgagggagc tattcatgcc aagtacgatg caattgataa agacactcca attcctacag 1381 atagacaaat tgaagtggat attcctcgct gtcatcagta cgatgaactg ttatcatcac 1441 cagaaggtca tgcaaaattt aggcgtgtat taaaagcctg ggtagtgtct catcctgatc 1501 ttgtgtattg gcaaggtctt gactcacttt gtgctccatt cctatatcta aacttcaata 1561 atgaagcctt ggcttatgca tgtatgtctg cttttattcc caaatacctg tataacttct 1621 tcttaaaaga caactcacat gtaatacaag agtatctgac tgtcttctct cagatgattg 1681 catttcatga tccagagctg agtaatcatc tcaatgagat tggtttcatt ccagatctct 1741 atgccatccc ttggtttctt accatgttta ctcatgtatt tccactacac aaaattttcc 1801 acctctggga taccttacta cttgggaatt cctctttccc attctgtatt ggagtagcaa 1861 ttcttcagca gctgcgggac cggcttttgg ctaatggctt taatgagtgt attcttctct 1921 tctccgattt accagaaatt gacattgaac gctgtgtgag agaatctatc aacctgtttt 1981 gttggactcc taaaagtgct acttacagac agcatgctca acctccaaag ccatcttctg 2041 acagcagtgg aggcagaagt tcggcacctt atttctctgc tgagtgtcca gatcctccaa 2101 agacagatct gtcaagagaa TCCATCCCAT TAAATGACCT GAAGTCAGAA GTATCACCAC 2161 GGATTTCAGC AGAGGACCTG ATTGACTTGT GTGAGCTCAC AGTGACAGGC CACTTCAAAA 2221 CACCCAGCAA GAAAACAAAG TCCAGTAAAC CAAAGCTCCT GGTGGTTGAC ATCCGGAATA 2281 GTGAAGACTT TATTCGTGGT CACATTTCAG GAAGCATCAA CATTCCATTC AGTGCTGCCT 2341 TCACTGCAGA AGGGGAGCTT ACCCAGGGCC CTTACACTGC TATGCTCCAG AACTTCAAAG 2401 GGAAGGTCAT TGTCATCGTG GGGCATGTGG CAAAACACAC AGCTGAGTTT GCAGCTCACC 2461 TTGTGAAGAT GAAATATCCA AGAATCTGTA TTCTAGATGG TGGCATTAAT AAAATAAAGC 2521 CAACAGGCCT CCTCACCATC CCATCTCCTC AAATATGCCC AACTTTCTTG TACAAAGTGG 2581 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2641 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2701 AGGGTTTGGC TTAGTACCAT GTGCCACGCG TTAAGTCgac aatcaacctc tggattacaa 2761 aatttgtgaa agatt