Construct: ORF TRCN0000480244
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006919.1_s317c1
- Derived from:
- ccsbBroadEn_15008
- DNA Barcode:
- CATGTGCGTCGTAGAGGGTCGTAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PMPCA (23203)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480244
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23203 | PMPCA | peptidase, mitochondrial pr... | NM_015160.3 | 99.5% | 2% | (many diffs) |
| 2 | human | 23203 | PMPCA | peptidase, mitochondrial pr... | XM_005266059.4 | 87.7% | 1.9% | (many diffs) |
| 3 | human | 23203 | PMPCA | peptidase, mitochondrial pr... | NM_001282946.1 | 80.5% | 2% | (many diffs) |
| 4 | human | 23203 | PMPCA | peptidase, mitochondrial pr... | NM_001282944.1 | 74.6% | 2.2% | (many diffs) |
| 5 | human | 23203 | PMPCA | peptidase, mitochondrial pr... | XM_017014543.2 | 64.9% | 2.5% | 0_1ins547;200_202delACCinsGTT;204_205delCA |
| 6 | human | 23203 | PMPCA | peptidase, mitochondrial pr... | XM_011518417.3 | 55.9% | .7% | (many diffs) |
| 7 | mouse | 66865 | Pmpca | peptidase (mitochondrial pr... | NM_173180.3 | 84.5% | .7% | (many diffs) |
| 8 | mouse | 66865 | Pmpca | peptidase (mitochondrial pr... | XM_006498237.3 | 60% | .7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 168
- ORF length:
- 99
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggctgtg gtgctggccg gngacgcggt tgctgcgggg ctcgggttct 121 tggggctgtt cgcggctgag gtttggacct cctgcgtaca gacggtttag tagtggtggt 181 gcctatccca acatccccct ctcttctccc ttacctggag tacccaagcc tgtttttgct 241 acagttgatg gacaggaaaa gtttgaaacc aaagtaacca cattggataa tgggcttcgc 301 gtggcatctc agaataagtt tggacagttt tgtacagtag gaattcttat caattcagga 361 tcgagatatg aagcgaaata ccttagtgga attgctcact ttttggaaaa attggcattt 421 tcgtctactg ctcgatttga cagcaaagat gaaattctgc ttacgttgga aaagcatggg 481 ggtatctgtg actgccagac atcaagagac accaccatgt atgctgtgtc tgctgatagc 541 aaaggcttgg acacggtggt tgccttactg gctgatgtgg ttctgcagcc ccggctaaca 601 gatgaagaag tcgagatgac gcggatggcg gtccagtttg agctggagga cctgaacctg 661 cggcctgacc cagagccact tctcaccgag atgattcatg aagcggctta cagggagaac 721 acagttggcc tccaccgttt ctgccccaca gaaaacgtag caaagatcaa ccgagaggtg 781 ctgcattcct acctgaggaa ctactacact cccggttgtg gtgctggccg gcgtgggcgt 841 ggagcacgag catctggtgg actgtgcccg gaagtacctc ctgggggtcc agccggcctg 901 ggggagcgca gaggccgtgg atattgacag atctgtggcc cagtacactg gggggattgc 961 caagctagaa agagacatgt ccaatgtcag cctgggcccg acccccatcc ccgagctcac 1021 gcacatcatg gttggactgg agagctgctc cttcctggag gaggacttca tcccctttgc 1081 agtgttgaac atgatgatgg gcggaggtgg ctccttctcg gctggtgggc ccggcaaggg 1141 catgttctcc aggctctacc tcaacgtgct caacaggcac cactggatgt ataacgcgac 1201 ctcctaccac cacagctacg aggacactgg cctcctttgc atccatgcca gcgccgaccc 1261 aagacaggtt cgagaaatgg tagaaatcaT CACAAAGGAG TTTATTTTAA TGGGCGGAAC 1321 CGTGGACACG GTGGAGCTGG AACGAGCCAA GACGCAGCTG ACATCAATGC TCATGATGAA 1381 CCTGGAATCC AGGCCTGTGA TCTTCGAGGA TGTGGGGAGG CAGGTGCTGG CCACTCGCTC 1441 CAGAAAGCTG CCGCACGAGC TGTGCACGCT CATCCGCAAC GTGAAGCCGG AAGATGTGAA 1501 GAGAGTCGCT TCTAAGATGC TCCGAGGGAA GCCGGCAGTG GCCGCCCTGG GTGACCTGAC 1561 TGACCTGCCC ACGTATGAGC ACATCCAGAC CGCCCTGTCG AGTAAGGACG GGCGCCTGCC 1621 CAGGACGTAC CGGCTCTTCC GGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 1681 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 1741 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA TGTGCGTCGT 1801 AGAGGGTCGT ACACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 1861 tt