Construct: ORF TRCN0000480244

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006919.1_s317c1
Derived from:
ccsbBroadEn_15008
DNA Barcode:
CATGTGCGTCGTAGAGGGTCGTAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PMPCA (23203)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480244
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23203 PMPCA peptidase, mitochondrial pr... NM_015160.3 99.5% 2% (many diffs)
2 human 23203 PMPCA peptidase, mitochondrial pr... XM_005266059.4 87.7% 1.9% (many diffs)
3 human 23203 PMPCA peptidase, mitochondrial pr... NM_001282946.1 80.5% 2% (many diffs)
4 human 23203 PMPCA peptidase, mitochondrial pr... NM_001282944.1 74.6% 2.2% (many diffs)
5 human 23203 PMPCA peptidase, mitochondrial pr... XM_017014543.2 64.9% 2.5% 0_1ins547;200_202delACCinsGTT;204_205delCA
6 human 23203 PMPCA peptidase, mitochondrial pr... XM_011518417.3 55.9% .7% (many diffs)
7 mouse 66865 Pmpca peptidase (mitochondrial pr... NM_173180.3 84.5% .7% (many diffs)
8 mouse 66865 Pmpca peptidase (mitochondrial pr... XM_006498237.3 60% .7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
168
ORF length:
99
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggctgtg gtgctggccg gngacgcggt tgctgcgggg ctcgggttct
121tggggctgtt cgcggctgag gtttggacct cctgcgtaca gacggtttag tagtggtggt
181gcctatccca acatccccct ctcttctccc ttacctggag tacccaagcc tgtttttgct
241acagttgatg gacaggaaaa gtttgaaacc aaagtaacca cattggataa tgggcttcgc
301gtggcatctc agaataagtt tggacagttt tgtacagtag gaattcttat caattcagga
361tcgagatatg aagcgaaata ccttagtgga attgctcact ttttggaaaa attggcattt
421tcgtctactg ctcgatttga cagcaaagat gaaattctgc ttacgttgga aaagcatggg
481ggtatctgtg actgccagac atcaagagac accaccatgt atgctgtgtc tgctgatagc
541aaaggcttgg acacggtggt tgccttactg gctgatgtgg ttctgcagcc ccggctaaca
601gatgaagaag tcgagatgac gcggatggcg gtccagtttg agctggagga cctgaacctg
661cggcctgacc cagagccact tctcaccgag atgattcatg aagcggctta cagggagaac
721acagttggcc tccaccgttt ctgccccaca gaaaacgtag caaagatcaa ccgagaggtg
781ctgcattcct acctgaggaa ctactacact cccggttgtg gtgctggccg gcgtgggcgt
841ggagcacgag catctggtgg actgtgcccg gaagtacctc ctgggggtcc agccggcctg
901ggggagcgca gaggccgtgg atattgacag atctgtggcc cagtacactg gggggattgc
961caagctagaa agagacatgt ccaatgtcag cctgggcccg acccccatcc ccgagctcac
1021gcacatcatg gttggactgg agagctgctc cttcctggag gaggacttca tcccctttgc
1081agtgttgaac atgatgatgg gcggaggtgg ctccttctcg gctggtgggc ccggcaaggg
1141catgttctcc aggctctacc tcaacgtgct caacaggcac cactggatgt ataacgcgac
1201ctcctaccac cacagctacg aggacactgg cctcctttgc atccatgcca gcgccgaccc
1261aagacaggtt cgagaaatgg tagaaatcaT CACAAAGGAG TTTATTTTAA TGGGCGGAAC
1321CGTGGACACG GTGGAGCTGG AACGAGCCAA GACGCAGCTG ACATCAATGC TCATGATGAA
1381CCTGGAATCC AGGCCTGTGA TCTTCGAGGA TGTGGGGAGG CAGGTGCTGG CCACTCGCTC
1441CAGAAAGCTG CCGCACGAGC TGTGCACGCT CATCCGCAAC GTGAAGCCGG AAGATGTGAA
1501GAGAGTCGCT TCTAAGATGC TCCGAGGGAA GCCGGCAGTG GCCGCCCTGG GTGACCTGAC
1561TGACCTGCCC ACGTATGAGC ACATCCAGAC CGCCCTGTCG AGTAAGGACG GGCGCCTGCC
1621CAGGACGTAC CGGCTCTTCC GGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA
1681GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT
1741TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA TGTGCGTCGT
1801AGAGGGTCGT ACACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga
1861tt

Download FASTA (ORF) (Full)