Transcript: Mouse NM_173180.3

Mus musculus peptidase (mitochondrial processing) alpha (Pmpca), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Pmpca (66865)
Length:
3125
CDS:
18..1592

Additional Resources:

NCBI RefSeq record:
NM_173180.3
NBCI Gene record:
Pmpca (66865)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_173180.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000305777 GGTAGAACCACTGGCATAAAT pLKO_005 1921 3UTR 100% 15.000 21.000 N Pmpca n/a
2 TRCN0000305778 TTTCGTCCACTGCTCGATTTG pLKO_005 364 CDS 100% 10.800 15.120 N Pmpca n/a
3 TRCN0000031681 CCCTGTAGAGAACATAGCGAA pLKO.1 689 CDS 100% 2.640 3.696 N Pmpca n/a
4 TRCN0000031683 GCCCAGATCATACCGGCTGTT pLKO.1 1565 CDS 100% 1.350 1.890 N Pmpca n/a
5 TRCN0000348990 TCACGATATGAAGCGAAATAT pLKO_005 306 CDS 100% 15.000 10.500 N Pmpca n/a
6 TRCN0000031680 CCCGACTGACAGATGAAGAAA pLKO.1 535 CDS 100% 5.625 3.938 N Pmpca n/a
7 TRCN0000324788 CCCGACTGACAGATGAAGAAA pLKO_005 535 CDS 100% 5.625 3.938 N Pmpca n/a
8 TRCN0000031679 CCTAGTAGAATGTGCCAGAAA pLKO.1 800 CDS 100% 4.950 3.465 N Pmpca n/a
9 TRCN0000324872 CCTAGTAGAATGTGCCAGAAA pLKO_005 800 CDS 100% 4.950 3.465 N Pmpca n/a
10 TRCN0000031682 GCAACAGTTGATGGACAGGAA pLKO.1 183 CDS 100% 2.640 1.848 N Pmpca n/a
11 TRCN0000051473 GCTCGATTTGACAGCAAAGAT pLKO.1 375 CDS 100% 5.625 3.938 N PMPCA n/a
12 TRCN0000290304 GCTCGATTTGACAGCAAAGAT pLKO_005 375 CDS 100% 5.625 3.938 N PMPCA n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_173180.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07853 pDONR223 100% 85.1% 91.2% None (many diffs) n/a
2 ccsbBroad304_07853 pLX_304 0% 85.1% 91.2% V5 (many diffs) n/a
3 TRCN0000477523 TTAAAATCGCCAGCACTACGCCTT pLX_317 22.7% 85.1% 91.2% V5 (many diffs) n/a
4 ccsbBroadEn_15008 pDONR223 54.9% 84.5% .7% None (many diffs) n/a
5 ccsbBroad304_15008 pLX_304 0% 84.5% .7% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000480244 CATGTGCGTCGTAGAGGGTCGTAC pLX_317 22.7% 84.5% .7% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV