Construct: ORF TRCN0000480260
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019004.2_s317c1
- Derived from:
- ccsbBroadEn_14688
- DNA Barcode:
- TAAGTGCCAAACCCGCCCCTCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LCK (3932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480260
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | NM_001042771.2 | 100% | 100% | |
| 2 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | NM_005356.5 | 100% | 100% | |
| 3 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | NM_001330468.2 | 89.9% | 89.7% | 631_632ins153 |
| 4 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | XM_024447046.1 | 89.7% | 89.7% | 186_359del |
| 5 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | XM_024447047.1 | 89.7% | 89.7% | 186_359del |
| 6 | human | 3932 | LCK | LCK proto-oncogene, Src fam... | XM_011541453.3 | 80.7% | 80.5% | 186_359del;805_806ins153 |
| 7 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | NM_001162433.1 | 88.6% | 96.8% | (many diffs) |
| 8 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | NM_010693.3 | 88.6% | 96.8% | (many diffs) |
| 9 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | XM_006502818.3 | 88.6% | 96.8% | (many diffs) |
| 10 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | XM_030253275.1 | 88.6% | 96.8% | (many diffs) |
| 11 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | XM_030253276.1 | 88.6% | 96.8% | (many diffs) |
| 12 | mouse | 16818 | Lck | lymphocyte protein tyrosine... | NM_001162432.1 | 86.7% | 94.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1596
- ORF length:
- 1527
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggctgtggc tgcagctcac acccggaaga tgactggatg gaaaacatcg 121 atgtgtgtga gaactgccat tatcccatag tcccactgga tggcaagggc acgctgctca 181 tccgaaatgg ctctgaggtg cgggacccac tggttaccta cgaaggctcc aatccgccgg 241 cttccccact gcaagacaac ctggttatcg ctctgcacag ctatgagccc tctcacgacg 301 gagatctggg ctttgagaag ggggaacagc tccgcatcct ggagcagagc ggcgagtggt 361 ggaaggcgca gtccctgacc acgggccagg aaggcttcat ccccttcaat tttgtggcca 421 aagcgaacag cctggagccc gaaccctggt tcttcaagaa cctgagccgc aaggacgcgg 481 agcggcagct cctggcgccc gggaacactc acggctcctt cctcatccgg gagagcgaga 541 gcaccgcggg atcgttttca ctgtcggtcc gggacttcga ccagaaccag ggagaggtgg 601 tgaaacatta caagatccgt aatctggaca acggtggctt ctacatctcc cctcgaatca 661 cttttcccgg cctgcatgaa ctggtccgcc attacaccaa tgcttcagat gggctgtgca 721 cacggttgag ccgcccctgc cagacccaga agccccagaa gccgtggtgg gaggacgagt 781 gggaggttcc cagggagacg ctgaagctgg tggagcggct gggggctgga cagttcgggg 841 aggtgtggat ggggtactac aacgggcaca cgaaggtggc ggtgaagagc ctgaagcagg 901 gcagcatgtc cccggacgcc ttcctggccg aggccaacct catgaagcag ctgcaacacc 961 agcggctggt tcggctctac gctgtggtca cccaggagcc catctacatc atcactgaat 1021 acatggagaa tgggagtcta gtggattttc tcaagacccc ttcaggcatc aagttgacca 1081 tcaacaaact cctggacatg gcagcccaaa ttgcagaagg catggcattc attgaagagc 1141 ggaattatat tcatcgtgac cttcgggctg ccaacattct ggtgtctgac accctgagct 1201 gcaagattgc agactttggc cTAGCACGCC TCATTGAGGA CAACGAGTAC ACAGCCAGGG 1261 AGGGGGCCAA GTTTCCCATT AAGTGGACAG CGCCAGAAGC CATTAACTAC GGGACATTCA 1321 CCATCAAGTC AGATGTGTGG TCTTTTGGGA TCCTGCTGAC GGAAATTGTC ACCCACGGCC 1381 GCATCCCTTA CCCAGGGATG ACCAACCCGG AGGTGATTCA GAACCTGGAG CGAGGCTACC 1441 GCATGGTGCG CCCTGACAAC TGTCCAGAGG AGCTGTACCA ACTCATGAGG CTGTGCTGGA 1501 AGGAGCGCCC AGAGGACCGG CCCACCTTTG ACTACCTGCG CAGTGTGCTG GAGGACTTCT 1561 TCACGGCCAC AGAGGGCCAG TACCAGCCTC AGCCTCCAAC TTTCTTGTAC AAAGTGGTTG 1621 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1681 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1741 AGTGCCAAAC CCGCCCCTCG TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1801 ttgtgaaaga tt