Construct: ORF TRCN0000480281
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018793.3_s317c1
- Derived from:
- ccsbBroadEn_15340
- DNA Barcode:
- ATGCACTGTTTCTGCACAACGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC653155 (653155)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480281
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NM_003913.5 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 2 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011410.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 3 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011411.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 4 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011413.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 5 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011414.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 6 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011415.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 7 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011416.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 8 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011417.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 9 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011418.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 10 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011420.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 11 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011421.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 12 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011422.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 13 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011423.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 14 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011424.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 15 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011425.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 16 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011426.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 17 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011427.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 18 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011428.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 19 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011430.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 20 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146784.2 | 8.8% | 1_2829del;3126C>T;3148_3582del | |
| 21 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146783.2 | 8.5% | 1_2829del;3126C>T;3148_3715del | |
| 22 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146785.2 | 8.3% | 1_2829del;3126C>T;3148_3792del | |
| 23 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | NM_013830.2 | 9.8% | 10.5% | (many diffs) |
| 24 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315425.1 | 9.8% | 10.5% | (many diffs) |
| 25 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315426.1 | 9.8% | 10.5% | (many diffs) |
| 26 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315427.1 | 9.8% | 10.5% | (many diffs) |
| 27 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315428.1 | 9.8% | 10.5% | (many diffs) |
| 28 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315429.1 | 9.8% | 10.5% | (many diffs) |
| 29 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315430.1 | 9.8% | 10.5% | (many diffs) |
| 30 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315431.1 | 9.8% | 10.5% | (many diffs) |
| 31 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315432.1 | 9.8% | 10.5% | (many diffs) |
| 32 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315433.1 | 9.8% | 10.5% | (many diffs) |
| 33 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315434.1 | 9.8% | 10.5% | (many diffs) |
| 34 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315435.1 | 9.8% | 10.5% | (many diffs) |
| 35 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315436.1 | 9.8% | 10.5% | (many diffs) |
| 36 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315437.1 | 9.8% | 10.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 411
- ORF length:
- 318
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGATCT CAAAGGAAAG ATGCCAAATA 121 AGATGATTCG AAAAGGTGTG TTCAAAGATC AGCATTTTGA TCAAAATCTC AACTTCATGT 181 ACATAGAAGT TGATAAAGTA ACAGAGAGGG AGAAAGTTAC TGTTATGAGC ACCATTAATC 241 CAACTAAGGA CCTGTTGGCT GACTTGATTG GGTGCCAGAG ACTTCCTGAA GACCAACGTA 301 AGAAAGTACA CCAGCTAAAG GACTTGTTGG ACCAGATTCT GATGTTGGAC CCAGCTAAAC 361 GAATTAGCAT CAACCAGGCC CTACAGCATG CCTTCATCCA GGAAAAAATT TTGCCAACTT 421 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 481 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 541 CTTGTGGAAA GGACGAATGC ACTGTTTCTG CACAACGACC ACGCGTTAAG TCgacaatca 601 acctctggat tacaaaattt gtgaaagatt