Construct: ORF TRCN0000480283
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018028.1_s317c1
- Derived from:
- ccsbBroadEn_15763
- DNA Barcode:
- AACCGTCATTTCTGCAGGACAAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ISCU (23479)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480283
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23479 | ISCU | iron-sulfur cluster assembl... | NM_213595.3 | 100% | 100% | |
| 2 | human | 23479 | ISCU | iron-sulfur cluster assembl... | NM_001301140.1 | 85.6% | 85.2% | (many diffs) |
| 3 | human | 23479 | ISCU | iron-sulfur cluster assembl... | NM_001320042.1 | 85.6% | 85.2% | (many diffs) |
| 4 | human | 23479 | ISCU | iron-sulfur cluster assembl... | NM_001301141.1 | 85.2% | 82.9% | (many diffs) |
| 5 | human | 23479 | ISCU | iron-sulfur cluster assembl... | NM_014301.4 | 83.1% | 77.8% | (many diffs) |
| 6 | human | 23479 | ISCU | iron-sulfur cluster assembl... | XM_011538101.2 | 76.6% | 66.4% | 339_340ins79;384_385ins38 |
| 7 | human | 23479 | ISCU | iron-sulfur cluster assembl... | XM_011538100.2 | 69.4% | 63.3% | (many diffs) |
| 8 | human | 23479 | ISCU | iron-sulfur cluster assembl... | NR_135127.1 | 21.3% | (many diffs) | |
| 9 | mouse | 66383 | Iscu | iron-sulfur cluster assembl... | NM_025526.4 | 87.7% | 95.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 567
- ORF length:
- 501
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggctggg gctttccgtc tgaggcgggc ggcatcggct ctgctgctgc 121 ggagcccccg cctgcccgcc cgggagctgt cggccccggc ccgactctat cacaagaagg 181 ttgttgatca ttatgaaaat cctagaaacg tggggtccct tgaCAAGACA TCTAAAAATG 241 TTGGAACTGG ACTGGTGGGG GCTCCAGCAT GTGGTGACGT AATGAAATTA CAGATTCAAG 301 TGGATGAAAA GGGGAAGATT GTGGATGCTA GGTTTAAAAC ATTTGGCTGT GGTTCCGCAA 361 TTGCCTCCAG CTCATTAGCC ACTGAATGGG TGAAAGGAAA GACGGTGGAG GAAGCCTTGA 421 CTATCAAAAA CACAGATATC GCCAAGGAGC TCTGCCTTCC TCCCGTGAAA CTGCACTGCT 481 CCATGCTGGC TGAAGATGCA ATCAAGGCCG CCCTGGCTGA TTACAAATTG AAACAAGAAC 541 CCAAAAAAGG AGAGGCAGAG AAGAAATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 601 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 661 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACCGTCA 721 TTTCTGCAGG ACAAGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 781 aagatt