Construct: ORF TRCN0000480284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017199.2_s317c1
- Derived from:
- ccsbBroadEn_02454
- DNA Barcode:
- TGAGGTAACATTATCGGCACGAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAP1 (10487)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480284
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_001105530.1 | 100% | 100% | |
| 2 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_001350475.1 | 100% | 100% | |
| 3 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_001350476.1 | 100% | 100% | |
| 4 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_001350477.2 | 100% | 100% | |
| 5 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_001350478.1 | 100% | 100% | |
| 6 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_001350479.1 | 100% | 100% | |
| 7 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_001350480.1 | 100% | 100% | |
| 8 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_001350481.1 | 100% | 100% | |
| 9 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_006367.3 | 100% | 100% | |
| 10 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_001330502.1 | 99.7% | 99.7% | 110_111insAGC |
| 11 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_001350482.1 | 99.7% | 99.7% | 110_111insAGC |
| 12 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_001350483.1 | 99.7% | 99.7% | 110_111insAGC |
| 13 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_001350484.1 | 99.7% | 99.7% | 110_111insAGC |
| 14 | human | 10487 | CAP1 | cyclase associated actin cy... | NM_001350485.1 | 99.7% | 99.7% | 110_111insAGC |
| 15 | human | 10487 | CAP1 | cyclase associated actin cy... | XM_011540510.1 | 99.5% | 98.7% | (many diffs) |
| 16 | human | 10487 | CAP1 | cyclase associated actin cy... | XM_017000069.1 | 99.5% | 98.7% | (many diffs) |
| 17 | human | 10487 | CAP1 | cyclase associated actin cy... | XM_011540515.1 | 99.3% | 98.5% | (many diffs) |
| 18 | mouse | 12331 | Cap1 | CAP, adenylate cyclase-asso... | NM_001301067.1 | 90.4% | 95.3% | (many diffs) |
| 19 | mouse | 12331 | Cap1 | CAP, adenylate cyclase-asso... | NM_007598.4 | 90.4% | 95.3% | (many diffs) |
| 20 | mouse | 12331 | Cap1 | CAP, adenylate cyclase-asso... | XM_006502695.1 | 90.4% | 95.3% | (many diffs) |
| 21 | mouse | 12331 | Cap1 | CAP, adenylate cyclase-asso... | XM_006502697.3 | 90.4% | 95.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1491
- ORF length:
- 1425
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgacatgcaa aatctggtag aaagattgga gagggcagtg ggccgcctgg 121 aggcagtatc tcatacctct gacatgcacc gtgggtatgc agacagtcct tcaaaagcag 181 gagcagctcc atatgtgcag gcatttgact cgctgcttgc tggtcctgtg gcagagtact 241 tgaagatcag taaagagatt gggggagacg tgcagaaaca tgcggagatg gtccacacag 301 gtttgaagtt ggagcgagct ctgttggtta cagcttctca gtgtcaacag ccagcagaaa 361 ataagctttc cgatttgttg gcacccatct cagagcagat caaagaagtg ataacctttc 421 gggagaagaa ccgaggcagc aagttgttta atcacctgtc agctgtcagc gaaagtatcc 481 aggccctggg ctgggtggct atggctccca agcctggccc ttatgtgaaa gaaatgaatg 541 atgccgccat gttttataca aaccgagtcc tcaaagagta caaagatgtg gataagaagc 601 atgtagactg ggtcaaagct tatttaagta tatggacaga gctgcaggct tacattaagg 661 agttccatac caccggactg gcctggagca aaacggggcc tgtggcaaaa gaactgagcg 721 gactgccatc tggaccctct gccggatcag gtcctcctcc ccctccacca ggcccccctc 781 ctcccccagt ctctaccagt tcaggctcag atgagtctgc ttcccgctca gcactgttcg 841 cgcagattaa tcagggggag agcattacac atgccctgaa acatgtatct gatgacatga 901 agactcacaa gaaccctgcc ctgaaggctc agagtggtcc agtacgcagt ggccccaaac 961 cattctctgc acctaaaccc caaaccagcc catcccccaa acgagccaca aagaaggagc 1021 cagctgtact tgaactggag ggcaagaagt ggagagtgga aaatcaggaa aatgtttcca 1081 acctggtgat tgaggacaca gagctgaaac aggtggctta catatacaag tgtgtcaaca 1141 cgacattgca aaTCAAGGGC AAAATTAACT CCATTACAGT AGATAACTGT AAGAAACTTG 1201 GCCTGGTATT CGATGACGTG GTGGGCATTG TGGAGATAAT CAACAGTAAG GATGTCAAAG 1261 TTCAGGTAAT GGGTAAAGTG CCAACCATAT CCATCAACAA AACAGATGGC TGCCATGCTT 1321 ACCTGAGCAA GAATTCCCTG GATTGTGAAA TAGTCAGTGC CAAATCTTCC GAGATGAATG 1381 TCCTCATTCC TACAGAAGGC GGTGACTTTA ATGAATTCCC AGTTCCTGAG CAGTTCAAGA 1441 CCCTATGGAA CGGGCAGAAG TTGGTCACCA CAGTGACAGA AATTGCTGGA TACCCAACTT 1501 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1561 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1621 CTTGTGGAAA GGACGATGAG GTAACATTAT CGGCACGAAG ACGCGTTAAG TCgacaatca 1681 acctctggat tacaaaattt gtgaaagatt