Construct: ORF TRCN0000480314
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017810.1_s317c1
- Derived from:
- ccsbBroadEn_07847
- DNA Barcode:
- GACAGTCTCCGACTGGGCACGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZCCHC14 (23174)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480314
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23174 | ZCCHC14 | zinc finger CCHC-type conta... | XM_005255858.3 | 97.4% | 97% | (many diffs) |
| 2 | human | 23174 | ZCCHC14 | zinc finger CCHC-type conta... | XM_017023082.2 | 92.8% | 92% | (many diffs) |
| 3 | human | 23174 | ZCCHC14 | zinc finger CCHC-type conta... | NM_015144.3 | 87.3% | 87.2% | 1_411del;2274C>T;2488G>A |
| 4 | human | 23174 | ZCCHC14 | zinc finger CCHC-type conta... | XR_243401.3 | 44.1% | (many diffs) | |
| 5 | human | 23174 | ZCCHC14 | zinc finger CCHC-type conta... | XM_011522964.2 | 36.4% | 33.7% | 1_411del;1475_1476ins128;1599_1600ins1531 |
| 6 | mouse | 142682 | Zcchc14 | zinc finger, CCHC domain co... | NM_080855.2 | 83.7% | 85.5% | (many diffs) |
| 7 | mouse | 142682 | Zcchc14 | zinc finger, CCHC domain co... | XM_006530667.3 | 72% | 73.2% | (many diffs) |
| 8 | mouse | 142682 | Zcchc14 | zinc finger, CCHC domain co... | XM_017312569.1 | 31.7% | 32.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2916
- ORF length:
- 2847
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctccaac cacccggcct tcagcttcca ccagaagcag gtgctgcgcc 121 aggagctcac gcagatccag agcagcctga acggcggcgg gggccacggc ggcaagggcg 181 cgcccgggcc gggcggcgcg ctgcccactt gcccagcctg ccacaagatc actccaagaa 241 ctgaggcccc tgtcagcagt gtcagtaata gtttggagaa tgccctgcac acatcagcac 301 attccacgga ggagtcgctg cccaagaggc ccttaggaaa acacagcaaa gtgagtgttg 361 aaaagataga cctgaaggga ttatcacaca caaaaaatga cagaaatgtt gaatgttcct 421 ttgaggtctt gtggtctgat tcttcaataa catcagtaac caaatcttcc tctgaagtga 481 cggaatttat ttcaaagcta tgtcagctct atcctgaaga gaacttggag aaactcattc 541 cttgcttagc tggtccggac gcattttatg tggagcgaaa ccacgtggat ctggactcag 601 gcctgaggta cctggcctca ttaccttctc acgtgttgaa aaatgaccat gtcaggaggt 661 ttctcagcac ttcctctccc ccacagcagc ttcagagtcc aagtcctggc aatccctccc 721 tttctaaagt aggtaccgtg atgggcgtgt ctggaaggcc tgtgtgtgga gtggctggta 781 tcccgtcctc gcagagcgga gcccagcacc acgggcagca cccggccggc tccgccgccc 841 ccttgcctca ctgctcccat gcgggcagcg cgggctcagc cctggcctac cggacccaga 901 tggacacatc acctgccatc ctcatgcctt ccagtctgca gacccctcag acccaggagc 961 agaatgggat tctagactgg cttaggaaac tgcgtttgca caagtattac cccgtcttta 1021 agcagctctc catggagaag tttttgagcc ttactgaaga agatctgaat aaatttgagt 1081 ctcttaccat gggggcaaag aagaagctca agacccagct ggagctggaa aaggagaagt 1141 cagagagacg gtgcctgaac ccctcggccc cgccgctggt caccagcagt ggtgtggctc 1201 gagtgccccc caccagccac gtcgggcccg tgcagtcggg gcggggcagc catgcagcag 1261 agctgcgggt ggaagtggag cagccccatc accagctgcc ccgggaaggc agttcctcgg 1321 agtactccag ctcctcctcc agccccatgg gggtacaggc ccgggaagag agctccgaca 1381 gcgctgagga gaatgacaga cgtgtggaga ttcacttgga gagctctgac aaggagaagc 1441 cggtgatgct gctgaatcac ttcacttcca gttccgccag acccacggcc caggttctcc 1501 ctgtgcagaa tgaggccagc tccaatccat caggccacca ccccctgccc ccgcagatgc 1561 tgagcgcagc ctcacacatc acacccatcc gcatgctgaa ttccgtgcac aagccggaaa 1621 gagggagcgc ggacatgaag ctcctctcgt cttctgtgca ctcacttttg tctctagaag 1681 aaaggaataa aggatctgga ccaagaagca gcatgaaagt ggacaagagc tttggcagcg 1741 ccatgatgga cgtgctgccc gcgtccgcac cccaccagcc tgtgcaggtc ctctctgggc 1801 tttcggagag cagctccatg tcacccacag tctcctttgg tccccggacc aaagtcgtgc 1861 atgcatccac gctggacagg gtgctgaaga cagcacagca accggccctg gtcgtggaga 1921 ccagcacggc tgccacgggg acgcccagca cagtcctcca cgccgcccgt ccgcccatca 1981 aactgctgct gtcgtcatct gttcctgctg attctgccat ttctgggcaa acttcctgtc 2041 ctaataatgt gcaaataagt gtgccccctg caataataaa cccccggact gctctgtaca 2101 cagccaacac caaagttgcc ttttctgcaa tgagcagtat gccaatgggc cccctgcagg 2161 gtggcttctg tgcaaacagc aacactgcct ctcccagcag ccacccctcc acgtcctttg 2221 ccaacatggc cacgttgccc agctgcccag cccccagctc cagcccggcg ctgtcctccg 2281 tccctgaaag cagtttctat agcagcagtg gcggtggcgg ctccacagga aacattcctg 2341 cctcgaatcc gaaccaccac caccaccacc accatcagca gcccccggca cccccgcagc 2401 ccgccccacc cccgccaggc tgcattgtgt gcacgtcctg tggctgcagc ggcagctgcg 2461 gctcgagtgg cctgactgtc agctacgcca actacttcca gcacccgttc tccggtccgt 2521 ccgtgttcac cttccccTTC TTGCCCTTCA GTCCCATGTG CAGCAGCGGC TACGTCAGCG 2581 CCCAGCAGTA CGGCGGCGGC TCCACCTTCC CCGTCGTGCA CGCCCCTTAC AGCAGCAGCG 2641 GGACCCCAGA CCCTGTCCTG AGTGGGCAGT CCACGTTTGC CGTGCCACCC ATGCAGAACT 2701 TCATGGCAGG GACAGCAGGG GTGTACCAGA CCCAAGGACT GGTGGGCAGT AGCAATGGTT 2761 CCAGTCACAA AAAGAGCGGG AACCTATCTT GTTACAACTG CGGGGCCACT GGTCACCGCG 2821 CCCAGGACTG CAAACAGCCG TCCATGGACT TCAACCGGCC AGGTACTTTT AGGTTGAAAT 2881 ACGCCCCTCC AGCAGAAAGT CTGGACTCCA CAGATTTGCC AACTTTCTTG TACAAAGTGG 2941 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3001 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3061 AGACAGTCTC CGACTGGGCA CGTGTACGCG TTAAGTCgac aatcaacctc tggattacaa 3121 aatttgtgaa agatt