Construct: ORF TRCN0000480320
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013651.1_s317c1
- Derived from:
- ccsbBroadEn_01205
- DNA Barcode:
- GTTTATTCAGGTGGAAAAAATGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIN1 (5300)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480320
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5300 | PIN1 | peptidylprolyl cis/trans is... | NM_006221.3 | 100% | 100% | |
| 2 | human | 5300 | PIN1 | peptidylprolyl cis/trans is... | XM_011528068.2 | 91.4% | 82% | (many diffs) |
| 3 | human | 5300 | PIN1 | peptidylprolyl cis/trans is... | NR_038830.2 | 66.8% | 1_27del;84_136del;570_731del | |
| 4 | human | 5301 | PIN1P1 | peptidylprolyl cis/trans is... | NR_023916.1 | 44.5% | (many diffs) | |
| 5 | human | 5300 | PIN1 | peptidylprolyl cis/trans is... | NR_038422.2 | 41% | 1_138del;195_247del;681_1191del | |
| 6 | mouse | 23988 | Pin1 | protein (peptidyl-prolyl ci... | NM_023371.3 | 87.5% | 95.1% | (many diffs) |
| 7 | mouse | 241593 | Pin1rt1 | protein (peptidyl-prolyl ci... | NM_001033768.2 | 86.9% | 92% | (many diffs) |
| 8 | mouse | 241593 | Pin1rt1 | protein (peptidyl-prolyl ci... | XM_006499488.1 | 86.9% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 555
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacgaggag aagctgccgc ccggctggga gaagcgcatg agccgcagct 121 caggccgagt gtactacttc aaccacatca ctaacgccag ccagtgggag cggcccagcg 181 gcaacagcag cagtggtggc aaaaacgggc agggggagcc tgccagggtc cgctgctcgc 241 acctgcTGGT GAAGCACAGC CAGTCACGGC GGCCCTCGTC CTGGCGGCAG GAGAAGATCA 301 CCCGGACCAA GGAGGAGGCC CTGGAGCTGA TCAACGGCTA CATCCAGAAG ATCAAGTCGG 361 GAGAGGAGGA CTTTGAGTCT CTGGCCTCAC AGTTCAGCGA CTGCAGCTCA GCCAAGGCCA 421 GGGGAGACCT GGGTGCCTTC AGCAGAGGTC AGATGCAGAA GCCATTTGAA GACGCCTCGT 481 TTGCGCTGCG GACGGGGGAG ATGAGCGGGC CCGTGTTCAC GGATTCCGGC ATCCACATCA 541 TCCTCCGCAC TGAGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 601 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 661 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTTTATTCAG GTGGAAAAAA 721 TGGGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt