Construct: ORF TRCN0000480373
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011256.1_s317c1
- Derived from:
- ccsbBroadEn_07739
- DNA Barcode:
- GATAAACCGGCTAAGACCGCAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WWP2 (11060)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480373
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11060 | WWP2 | WW domain containing E3 ubi... | NM_001270454.2 | 99.8% | 100% | 1239G>A;1707T>C;2091A>G |
| 2 | human | 11060 | WWP2 | WW domain containing E3 ubi... | NM_007014.5 | 99.8% | 100% | 1239G>A;1707T>C;2091A>G |
| 3 | human | 11060 | WWP2 | WW domain containing E3 ubi... | XM_011522823.2 | 99.8% | 100% | 1239G>A;1707T>C;2091A>G |
| 4 | human | 11060 | WWP2 | WW domain containing E3 ubi... | XM_011522825.1 | 99.8% | 100% | 1239G>A;1707T>C;2091A>G |
| 5 | human | 11060 | WWP2 | WW domain containing E3 ubi... | XM_017022879.1 | 99.8% | 100% | 1239G>A;1707T>C;2091A>G |
| 6 | human | 11060 | WWP2 | WW domain containing E3 ubi... | XM_017022880.1 | 99.8% | 100% | 1239G>A;1707T>C;2091A>G |
| 7 | human | 11060 | WWP2 | WW domain containing E3 ubi... | XM_017022881.1 | 99.8% | 100% | 1239G>A;1707T>C;2091A>G |
| 8 | human | 11060 | WWP2 | WW domain containing E3 ubi... | NM_001270453.1 | 86.5% | 86.6% | (many diffs) |
| 9 | human | 11060 | WWP2 | WW domain containing E3 ubi... | XM_011522826.3 | 86.5% | 86.6% | (many diffs) |
| 10 | human | 11060 | WWP2 | WW domain containing E3 ubi... | NM_199424.3 | 49.4% | 49.5% | 0_1ins1317;390T>C;774A>G |
| 11 | human | 11060 | WWP2 | WW domain containing E3 ubi... | NM_001270455.2 | 38.4% | 38.5% | 1005_1005delGins1606 |
| 12 | mouse | 66894 | Wwp2 | WW domain containing E3 ubi... | NM_025830.3 | 89.8% | 95.9% | (many diffs) |
| 13 | mouse | 66894 | Wwp2 | WW domain containing E3 ubi... | XM_006531299.3 | 89.8% | 95.9% | (many diffs) |
| 14 | mouse | 66894 | Wwp2 | WW domain containing E3 ubi... | XR_001778461.1 | 74.8% | (many diffs) | |
| 15 | mouse | 66894 | Wwp2 | WW domain containing E3 ubi... | XM_006531301.1 | 45.1% | 48.7% | (many diffs) |
| 16 | mouse | 66894 | Wwp2 | WW domain containing E3 ubi... | XM_017312941.1 | 33.7% | 35.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2676
- ORF length:
- 2610
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc atctgccagc tctagccggg caggagtggc cctgcctttt gagaagtctc 121 agctcacttt gaaagtggtg tccgcaaagc ccaaggtgca taatcgtcaa cctcgaatta 181 actcctacgt ggaggtggcg gtggatggac tccccagtga gaccaagaag actgggaagc 241 gcattgggag ctctgagctt ctctggaatg agatcatcat tttgaatgtc acggcacaga 301 gtcatttaga tttaaaggtc tggagctgcc ataccttgag aaatgaactg ctaggcaccg 361 catctgtcaa cctctccaac gtcttgaaga acaatggggg caaaatggag aacatgcagc 421 tgaccctgaa cctgcagacg gagaacaaag gcagcgttgt ctcaggcgga gagctgacaa 481 ttttcctgga cgggccaact gttgatctgg gaaatgtgcc taatggcagt gccctgacag 541 atggatcaca gctgccttcg agagactcca gtggaacagc agtagctcca gagaaccggc 601 accagccccc cagcacaaac tgctttggtg gaagatcccg gacgcacaga cattcgggtg 661 cttcagccag aacaacccca gcaaccggcg agcaaagccc cggtgctcgg agccggcacc 721 gccagcccgt caagaactca ggccacagtg gcttggccaa tggcacagtg aatgatgaac 781 ccacaacagc cactgatccc gaagaacctt ccgttgttgg tgtgacgtcc ccacctgctg 841 cacccttgag tgtgaccccg aatcccaaca cgacttctct ccctgcccca gccacaccgg 901 ctgaaggaga ggaacccagc acttcgggta cacagcagct cccagcggct gcccaggccc 961 ccgacgctct gcctgctgga tgggaacagc gagagctgcc caacggacgt gtctattatg 1021 ttgaccacaa taccaagacc accacctggg agcggcccct tcctccaggc tgggaaaaac 1081 gcacagatcc ccgaggcagg ttttactatg tggatcacaa tactcggacc accacctggc 1141 agcgtccgac cgcggagtac gtgcgcaact atgagcagtg gcagtcgcag cggaatcagc 1201 tccagggggc catgcagcac ttcagccaaa gattcctcta ccagtcttcg agtgcttcga 1261 ctgaccatga tcccctgggc cccctccctc ctggctggga gaaaagacag gacaatggac 1321 gggtgtatta cgtgaaccat aacactcgca cgacccagtg ggaggatccc cggacccagg 1381 ggatgatcca ggaaccagct ctgcccccag gatgggagat gaaatacacc agcgaggggg 1441 tgcgatactt tgtggaccac aatacccgca ccaccacctt taaggatcct cgcccggggt 1501 ttgagtcggg gacgaagcaa ggttcccctg gtgcttatga ccgcagtttt cggtggaagt 1561 atcaccagtt ccgtttcctc tgccattcaa atgccctacc tagccacgtg aagatcagcg 1621 tttccaggca gacgcttttc gaagattcct tccaacagat catgaacatg aaaccctatg 1681 acctgcgccg ccggctctac atcatcatgc gtggcgagga gggcctggac tatgggggca 1741 tcgccagaga gtggtttttc ctcctgtctc acgaggtgct caaccctatg tattgtttat 1801 ttgaatatgc cggaaagaac aattactgcc tgcagatcaa ccccgcctcc tccatcaacc 1861 cggaccacct cacctacttt cgctttatag gcagattcat cgccatggcg ctgtaccatg 1921 gaaagttcat cgacacgggc ttcaccctcc ctttctacaa gcggatgctc aataagagac 1981 caaccctgaa agacctggag tccattgacc ctgagttcta caactccatt gtctggatca 2041 aagagaacaa cctggaagaa tgtggcctgg agctgtactt catccaggac atggagatac 2101 tgggcaaggt gacgacccac gagctgaagg agggcggcga gagcatccgg gtcacggagg 2161 agaacaagga agagtacatc atgctgctga ctgactGGCG TTTCACCCGA GGCGTGGAAG 2221 AGCAGACCAA AGCCTTCCTG GATGGCTTCA ACGAGGTGGC CCCGCTGGAG TGGCTGCGCT 2281 ACTTTGACGA GAAAGAGCTG GAGCTGATGC TGTGCGGCAT GCAGGAGATA GACATGAGCG 2341 ACTGGCAGAA GAGCACCATC TACCGGCACT ACACCAAGAA CAGCAAGCAG ATCCAGTGGT 2401 TCTGGCAGGT GGTGAAGGAG ATGGACAACG AGAAGAGGAT CCGGCTGCTG CAGTTTGTCA 2461 CCGGTACCTG CCGCCTGCCC GTCGGGGGAT TTGCCGAACT CATCGGTAGC AACGGACCAC 2521 AGAAGTTTTG CATTGACAAA GTTGGCAAGG AAACCTGGCT GCCCAGAAGC CACACCTGCT 2581 TCAACCGTCT GGATCTTCCA CCCTACAAGA GCTACGAACA GCTGAGAGAG AAGCTGCTGT 2641 ATGCCATTGA GGAGACCGAG GGCTTTGGAC AGGAGTACCC AACTTTCTTG TACAAAGTGG 2701 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2761 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2821 AGATAAACCG GCTAAGACCG CAGGCACGCG TTAAGTCgac aatcaacctc tggattacaa 2881 aatttgtgaa agatt