Construct: ORF TRCN0000480376
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009712.1_s317c1
- Derived from:
- ccsbBroadEn_11189
- DNA Barcode:
- CTCTATCCCCGCCTGTTCGCGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TK2 (7084)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480376
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7084 | TK2 | thymidine kinase 2 | NM_001271934.2 | 99.8% | 99.5% | 410C>A |
| 2 | human | 7084 | TK2 | thymidine kinase 2 | NM_001172643.1 | 91.8% | 90.5% | (many diffs) |
| 3 | human | 7084 | TK2 | thymidine kinase 2 | NM_004614.5 | 81.1% | 80% | (many diffs) |
| 4 | human | 7084 | TK2 | thymidine kinase 2 | NM_001172644.2 | 80.9% | 71.2% | (many diffs) |
| 5 | human | 7084 | TK2 | thymidine kinase 2 | NM_001272050.2 | 77.6% | 77.3% | 0_1ins144;266C>A |
| 6 | human | 7084 | TK2 | thymidine kinase 2 | NM_001172645.1 | 74.3% | 73.2% | (many diffs) |
| 7 | human | 7084 | TK2 | thymidine kinase 2 | NM_001271935.1 | 54.4% | 40.9% | (many diffs) |
| 8 | human | 7084 | TK2 | thymidine kinase 2 | NR_073520.2 | 11.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 717
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacaagtatc tgtgtcgagg gcaatattgc aagtgggaag acgacatgcc 121 tggaattctt ctccaacgcg acagacgtcg aggtgttaac ggagcctgtg tccaagtgga 181 gaaatgtccg tggccacaat cctctgggcc tgatgtacca cgatgcctct cgctggggtc 241 ttacgctaca gacttatgtg cagctcacca tgctggacag gcatactcgt ccTCAGGTGT 301 CATCTGTACG GTTGATGGAG AGGTCGATTC ACAGCGCAAG ATACATTTTT GTAGAAAACC 361 TGTATAGAAG TGGGAAGATG CCAGAAGTGG ACTATGTAGT TCTGTCGGAA TGGTTTGACT 421 GGATCTTGAG GAACATGGAC GTGTCTGTTG ATTTGATAGT TTACCTTCGG ACCAATCATG 481 AGACTTGTTA CCAGAGGTTA AAGAAGAGAT GCAGGGAAGA GGAGAAGGTC ATTCCGCTGG 541 AATACCTGGA AGCAATTCAC CATCTCCATG AGGAGTGGCT CATCAAAGGC AGCCTTTTCC 601 CCATGGCAGC CCCTGTTCTG GTGATTGAGG CTGACCACCA CATGGAGAGG ATGTTAGAAC 661 TCTTTGAACA AAATCGGGAT CGAATATTAA CTCCAGAGAA TCGGAAGCAT TGCCCATTGC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GACTCTATCC CCGCCTGTTC GCGTGCACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt