Construct: ORF TRCN0000480391
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018877.2_s317c1
- Derived from:
- ccsbBroadEn_14910
- DNA Barcode:
- GTAAAGCGGTTGTCTCGACGCCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RIPK1 (8737)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480391
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8737 | RIPK1 | receptor interacting serine... | NM_001354930.2 | 99.6% | 37% | (many diffs) |
| 2 | human | 8737 | RIPK1 | receptor interacting serine... | NM_003804.6 | 99.6% | 37% | (many diffs) |
| 3 | human | 8737 | RIPK1 | receptor interacting serine... | NM_001354931.2 | 92.7% | 30.3% | (many diffs) |
| 4 | human | 8737 | RIPK1 | receptor interacting serine... | NM_001317061.3 | 75.4% | 13.1% | (many diffs) |
| 5 | human | 8737 | RIPK1 | receptor interacting serine... | NM_001354932.2 | 75.4% | 13.1% | (many diffs) |
| 6 | human | 8737 | RIPK1 | receptor interacting serine... | NM_001354933.2 | 75.4% | 13.1% | (many diffs) |
| 7 | human | 8737 | RIPK1 | receptor interacting serine... | NM_001354934.2 | 75.4% | 13.1% | (many diffs) |
| 8 | human | 8737 | RIPK1 | receptor interacting serine... | XM_017011403.1 | 75.4% | 13.1% | (many diffs) |
| 9 | human | 8737 | RIPK1 | receptor interacting serine... | XM_017011404.2 | 56.1% | 1.6% | (many diffs) |
| 10 | human | 8737 | RIPK1 | receptor interacting serine... | XM_006715237.3 | 50% | 68.9% | (many diffs) |
| 11 | human | 8737 | RIPK1 | receptor interacting serine... | XM_017011405.1 | 50% | 68.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 909
- ORF length:
- 840
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaaccagac atgtccttga atgtcattaa gatgaaatcc agtgacttcc 121 tggagagtgc agaactggac agcggaggct tcgggaaggt gtctctgtgt ttccacagaa 181 cccagggact catgatcatg aaaacagtgt acaaggggcc caactgcatt gagcacaacg 241 aggccctctt ggaggaggcg aagatgatga acagactgag acacagccgg gtggtgaagc 301 tcctgggcgt catcatagag gaagggaagt actccctggt gatggagtac atggagaagg 361 gcaacctgat gcacgtgctg aaagccgaga tgagtactcc gctttctgta aaaggaagga 421 taattttgga aatcattgaa ggaatgtgct acttacatgg aaaaggcgtg atacacaagg 481 acctgaagcc tgaaaatatc cttgttgata atgacttcca cattaagatc gcagacctcg 541 gccttgcctc ctttaagatg tggagcaaac tgaataatga agagcacaat gagctgaggg 601 aagtggacgg caccgctaag aagaatggcg gcaccctcta ctacatggcg cccgagcacc 661 tgaatgacgt caacgcaaag cccacagaga agtcggatgt gtacagcttt gctgtagtac 721 tctgggcgat atttgcaaat aaggagccat atgaaaatgc tatctgtgag cagcagttga 781 taatgtgcat aaaatctggg aacaggccag atgtgatgac atcactgagt actgcccaga 841 gaaattatca gtctcatgaa gctctgctgg gaagcgaatc cggaagctcg gccgacattt 901 cctggcattg aagaaaaatt taggcctttt tatttaagtc aattagaaga aagtgtagaa 961 gaggacgtga agagtttaaa gaaagagtat tcaaacgaaa atgcagttgt gaagagaatg 1021 cagtctcttc aacttgattg tgtggcagta ccttcaagcc ggtcaaattc agccacagaa 1081 cagcctggtt cactgcacag ttcccaggga cttgggatgg gtcctgtgga ggagtcctgg 1141 tttgctcctt ccctggagca cccacaagaa gagaatgagc ccagcctgca gagtaaactc 1201 caagacgaag ccaactacca tctttatggc agccgcatgg acaggcagac gaaacagcag 1261 cccagacaga attgggctac aacagagagg aggaaaggag acgcagggtc tcccatgacc 1321 tttttgcaca gcaaagacct tacgagaatt ttcagaatac agagggaaaa ggcactgctt 1381 attccagtgc agccagtcat ggtaatgcag tgcaccagcc ctcagggctc accagccaac 1441 ctcaagtact gtatcagaac aatggattat atagctcaca tggctttgga acaagaccac 1501 tggatccagg aacagcaggt cccagagttt ggtacaggcc aattccaagt catatgccta 1561 gtctgcataa tatcccagtg cctgagacca actatctagg aaatacaccc accatgccat 1621 tcagctcctt gccaccaaca gatgaatcta taaaatatac catatacaat agtactggca 1681 ttcagattgg agcctacaat TATATGGAGA TTGGTGGGAC GAGTTCATCA CTACTAGACA 1741 GCACAAATAC GAACTTCAAA GAAGAGCCAG CTGCTAAGTA CCAAGCTATC TTTGATAATA 1801 CCACTAGTCT GACGGATAAA CACCTGGACC CAATCAGGGA AAATCTGGGA AAGCACTGGA 1861 AAAACTGTGC CCGTAAACTG GGCTTCACAC AGTCTCAGAT TGATGAAATT GACCATGACT 1921 ATGAGCGAGA TGGACTGAAA GAAAAGGTTT ACCAGATGCT CCAAAAGTGG GTGATGAGGG 1981 AAGGCATAAA GGGAGCCACG GTGGGGAAGC TGGCCCAGGC GCTCCACCAG TGTTCCAGGA 2041 TCGACCTTCT GAGCAGCTTG ATTTACGTCA GCCAGAACTT GCCAACTTTC TTGTACAAAG 2101 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2161 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2221 ACGAGTAAAG CGGTTGTCTC GACGCCAAAC GCGTTAAGTC gacaatcaac ctctggatta 2281 caaaatttgt gaaagatt