Construct: ORF TRCN0000480476
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005588.1_s317c1
- Derived from:
- ccsbBroadEn_02368
- DNA Barcode:
- TTACTCAGGGATTCTGAGAATTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIMM17B (10245)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480476
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10245 | TIMM17B | translocase of inner mitoch... | NM_005834.5 | 99.8% | 100% | 228C>T |
| 2 | human | 10245 | TIMM17B | translocase of inner mitoch... | NM_001167947.2 | 77.3% | 77.4% | 127_276del;378C>T |
| 3 | mouse | 21855 | Timm17b | translocase of inner mitoch... | NM_011591.5 | 89.3% | 95.9% | (many diffs) |
| 4 | mouse | 21855 | Timm17b | translocase of inner mitoch... | NM_001313693.1 | 77.5% | 83.1% | (many diffs) |
| 5 | mouse | 21855 | Timm17b | translocase of inner mitoch... | XM_017318459.1 | 77.2% | 82.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagtacgct cgggagccct gcccatggcg aattgtggat gattgcggtg 121 gagccttcac tatgggtgtc atcggtggcg gagTCTTCCA GGCCATCAAG GGTTTCCGCA 181 ATGCCCCTGT TGGAATTCGG CACCGGTTGA GAGGTAGTGC CAATGCTGTG AGGATCCGAG 241 CCCCCCAGAT TGGAGGTAGC TTCGCAGTGT GGGGGGGCCT GTTCTCCACC ATTGACTGTG 301 GCCTGGTGCG GCTTCGGGGC AAGGAGGATC CCTGGAACTC TATCACCAGT GGAGCATTGA 361 CCGGGGCTGT GCTGGCTGCC CGCAGTGGCC CACTGGCCAT GGTGGGCTCA GCAATGATGG 421 GGGGCATCCT GTTGGCCCTC ATTGAGGGCG TTGGCATCCT CCTCACTCGC TACACAGCCC 481 AGCAGTTCCG AAATGCGCCC CCATTCCTGG AGGACCCCAG CCAGCTGCCC CCTAAGGATG 541 GCACCCCGGC CCCAGGCTAC CCCAGCTATC AGCAGTACCA CTGCCCAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGATTA CTCAGGGATT CTGAGAATTT CACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t