Construct: ORF TRCN0000480492
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016083.1_s317c1
- Derived from:
- ccsbBroadEn_05437
- DNA Barcode:
- GAGTTGGTACACAGAGCCTTCACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB7B (338382)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480492
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 338382 | RAB7B | RAB7B, member RAS oncogene ... | NM_001164522.3 | 100% | 100% | |
| 2 | human | 338382 | RAB7B | RAB7B, member RAS oncogene ... | NM_177403.5 | 100% | 100% | |
| 3 | human | 338382 | RAB7B | RAB7B, member RAS oncogene ... | XM_006711288.4 | 89.8% | 88.9% | (many diffs) |
| 4 | human | 338382 | RAB7B | RAB7B, member RAS oncogene ... | NM_001304839.2 | 78.8% | 78.8% | 395_396ins126 |
| 5 | mouse | 226421 | Rab7b | RAB7B, member RAS oncogene ... | NM_145509.3 | 85.4% | 88.4% | (many diffs) |
| 6 | mouse | 226421 | Rab7b | RAB7B, member RAS oncogene ... | XM_006529430.2 | 85.4% | 88.4% | (many diffs) |
| 7 | mouse | 226421 | Rab7b | RAB7B, member RAS oncogene ... | NM_001311096.1 | 68% | 70.8% | (many diffs) |
| 8 | mouse | 226421 | Rab7b | RAB7B, member RAS oncogene ... | XM_006529428.3 | 66.1% | 68.4% | (many diffs) |
| 9 | mouse | 226421 | Rab7b | RAB7B, member RAS oncogene ... | XM_006529433.3 | 54.9% | 54.7% | (many diffs) |
| 10 | mouse | 226421 | Rab7b | RAB7B, member RAS oncogene ... | XM_006529429.3 | 52.6% | 54.8% | (many diffs) |
| 11 | mouse | 226421 | Rab7b | RAB7B, member RAS oncogene ... | XM_006529431.3 | 42.5% | 42.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 663
- ORF length:
- 597
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tccccggaag aaggtggacc tgaaactcat tatcgtcgga gccattggtg 121 tgggaaagac ctccctcctt caccaatatg tgcacaagac gttttatgag gaataccaga 181 ccacactggg ggccagcatc ctctccaaga ttatcatatt gggtgacaca actttGAAGT 241 TACAGATCTG GGACACGGGC GGTCAGGAGC GGTTCCGCTC CATGGTGTCC ACGTTCTACA 301 AGGGCTCCGA TGGCTGCATC CTAGCTTTTG ATGTCACCGA CCTGGAGTCT TTTGAAGCCC 361 TGGATATCTG GCGGGGTGAT GTCCTGGCCA AGATTGTCCC CATGGAGCAG TCCTACCCCA 421 TGGTGTTGTT GGGGAACAAG ATCGATCTGG CAGACCGGAA GGTACCCCAG GAAGTAGCTC 481 AAGGCTGGTG TAGAGAGAAA GATATTCCTT ACTTTGAAGT CAGTGCCAAG AATGACATCA 541 ATGTGGTGCA AGCGTTTGAG ATGCTGGCCA GTAGGGCTCT GTCGAGGTAC CAGAGCATCT 601 TAGAAAATCA CCTCACAGAA TCCATCAAGC TCTCGCCAGA CCAGTCAAGG AGCAGATGCT 661 GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 781 GGCTTTATAT ATCTTGTGGA AAGGACGAGA GTTGGTACAC AGAGCCTTCA CAACGCGTTA 841 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt