Construct: ORF TRCN0000480506
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005865.1_s317c1
- Derived from:
- ccsbBroadEn_09644
- DNA Barcode:
- ACTTAACCGTCAGCACACAATTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C18orf25 (147339)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480506
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 147339 | C18orf25 | chromosome 18 open reading ... | NM_145055.5 | 99.8% | 99.5% | 6G>T;1198C>A |
| 2 | human | 147339 | C18orf25 | chromosome 18 open reading ... | XM_011525822.2 | 99.8% | 99.5% | 6G>T;1198C>A |
| 3 | human | 147339 | C18orf25 | chromosome 18 open reading ... | NM_001008239.2 | 84.7% | 84.4% | 6G>T;712_713ins183;1015C>A |
| 4 | human | 147339 | C18orf25 | chromosome 18 open reading ... | XM_017025555.1 | 84.7% | 84.4% | 6G>T;712_713ins183;1015C>A |
| 5 | mouse | 212163 | 8030462N17Rik | RIKEN cDNA 8030462N17 gene | NM_178670.3 | 90.9% | 93.5% | (many diffs) |
| 6 | mouse | 212163 | 8030462N17Rik | RIKEN cDNA 8030462N17 gene | XM_006526426.1 | 90.9% | 93.5% | (many diffs) |
| 7 | mouse | 212163 | 8030462N17Rik | RIKEN cDNA 8030462N17 gene | XM_011247067.2 | 81.6% | 76% | (many diffs) |
| 8 | mouse | 212163 | 8030462N17Rik | RIKEN cDNA 8030462N17 gene | XM_011247069.2 | 77.4% | 80.6% | (many diffs) |
| 9 | mouse | 212163 | 8030462N17Rik | RIKEN cDNA 8030462N17 gene | XM_011247070.2 | 68.3% | 63.8% | (many diffs) |
| 10 | mouse | 212163 | 8030462N17Rik | RIKEN cDNA 8030462N17 gene | XM_006526428.2 | 37.7% | 37.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1278
- ORF length:
- 1212
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tatggaggag gcagtgggaa aagttgaaga actcattgag tccgaagccc 121 caccaaaagc atctgaacaa gagacagcca aggaggaaga tggatctgta gaactggaat 181 ctcaagttca gaaagatggt gtagcggatt ctacagttat ttcttcaatg ccctgcttgt 241 tgatggaact gagaagggac tcttctgagt ctcagttagc atccacagag agtgacaagc 301 ctacaactgg ccgagtttat gagagtgact cctctaatca ctgcatgctt tccccttcct 361 ctagtggtca cctggctgat tcagatacgt tgtcttccgc agaagagaat gaaccctctc 421 aggcagaaac ggcggtagaa ggagaccctt caggagtgtc tggtgccaca gttgggcgca 481 agtctaggcg gtcccgatct gaaagtgaaa cttccactat ggctgccaag aaaaaccggc 541 aatccagtga taaacagaat ggccgagtcg ccaaggttaa aggtcatcgg agccaaaagc 601 acaaggagag gatcaggcta ctgaggcaga aacgggaggc tgctgcaagg aagaaatata 661 acctgctgca ggacagtagt accagtgata gtgacctgac ttgtgactca agcacgagct 721 catcagatga tgatgaagag gtttcaggga gcagcaagac aatcactgca gagataccag 781 atggacctcc agttgtagct cattatgata tgtctgacac caactcTGAC CCAGAAGTGG 841 TAAATGTGGA CAATTTATTG GCGGCTGCAG TAGTTCAAGA GCACAGTAAT TCTGTAGGCG 901 GCCAGGACAC AGGAGCTACC TGGAGGACCA GCGGGCTTCT AGAGGAGCTG AATGCAGAGG 961 CAGGTCATTT GGATCCAGGA TTCCTAGCAA GTGACAAAAC ATCTGCTGGC AATGCGCCAC 1021 TCAATGAAGA AATTAACATT GCGTCTTCAG ATAGTGAAGT AGAGATTGTG GGAGTTCAGG 1081 AACATGCAAG GTGTGTTCAT CCTCGAGGTG GTGTGATTCA GAGTGTTTCT TCATGGAAGC 1141 ATGGCTCGGG CACGCAGTAT GTTAGCACCA GGCAAACACA GTCATGGACT GCTGTGACTC 1201 CCCAGCAGAC TTGGGCTTCA CCAGCAGAAG TTGTTGACCT TACCTTGGAT GAGGATAGCA 1261 GGAGTAAATA CCTACTGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1321 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1381 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACTTAAC CGTCAGCACA 1441 CAATTTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt