Construct: ORF TRCN0000480529
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013810.2_s317c1
- Derived from:
- ccsbBroadEn_10983
- DNA Barcode:
- CACGTTTAACACTTGGCGGCCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYO1D (4642)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480529
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4642 | MYO1D | myosin ID | NM_001303280.2 | 100% | 100% | |
| 2 | human | 4642 | MYO1D | myosin ID | XM_017024685.2 | 45.4% | 45.2% | 1296_1297insGTGGG;1301delA;1305_2865del |
| 3 | human | 4642 | MYO1D | myosin ID | NM_001303279.2 | 45.1% | 44.9% | 1296_1297insGTGGG;1301delA;1305_2883del |
| 4 | human | 4642 | MYO1D | myosin ID | NM_015194.3 | 43.1% | 42.9% | 1296_1297insGTGGG;1301delA;1305_3018del |
| 5 | human | 4642 | MYO1D | myosin ID | XR_001752521.2 | 37.6% | (many diffs) | |
| 6 | human | 4642 | MYO1D | myosin ID | XR_934470.2 | 36.3% | (many diffs) | |
| 7 | human | 4642 | MYO1D | myosin ID | XM_024450770.1 | 34.3% | 34.1% | (many diffs) |
| 8 | mouse | 338367 | Myo1d | myosin ID | XM_011249086.2 | 78.2% | 85.8% | (many diffs) |
| 9 | mouse | 338367 | Myo1d | myosin ID | XM_006533586.3 | 74.5% | 80.4% | (many diffs) |
| 10 | mouse | 338367 | Myo1d | myosin ID | XM_006533585.1 | 41.2% | 44.5% | (many diffs) |
| 11 | mouse | 338367 | Myo1d | myosin ID | NM_177390.3 | 38.7% | 41.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1374
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagcaggag agcctggaat tcggcaaggc agacttcgtg ctgatggaca 121 ccgtctccat gcccgagttc atggccaacc tcaggctcag atttgaaaaa gggcgcatct 181 atacgttcat tggagaagtc gtcgtttctg tgaaccctta caagttgttg aacatctatg 241 gaagagacac aattgagcag tataaaggcc gtgagctgta tgagagaccg cctcaccttt 301 ttgctattgc ggatgctgct tacaaggcta tgaagaggcg atcaaaagac acttgtattg 361 tgatatcagg ggaaagtgga gctggtaaaa cggaagccag taagtacatt atgcagtata 421 ttgcggccat caccaacccc agtcagagag cagaggttga aagagtgaag aatatgttgc 481 ttaagtccaa ctgtgttttg gaagcttttg gaaatgccaa aaccaaccgt aatgacaact 541 caagcaggtt tggaaaatac atggatatca actttgactt caagggtgac cctattggtg 601 ggcatatcaa taactactta ctagaaaagt ctcgagtgat tgtgcaacag ccaggagaaa 661 gaagctttca ttctttctat cagctactcc aaggaggttc agaacaaatg ctacgctctc 721 tacatctcca gaaatccctt tcatcctaca actatattca tgtgggagct caattaaagt 781 cttctatcaa tgatgctgcc gaattcagag ttgttgctga tgccatgaaa gtcattggct 841 tcaaacctga ggagatccaa acagtgtata agattttggc tgctattctg cacttgggaa 901 atttaaaatt tgtagtagat ggtgacacgc ctcttattga gaatggcaaa gtagtatcta 961 tcatagcaga attgctctct acTAAGACAG ATATGGTTGA GAAAGCCCTT CTTTACCGGA 1021 CTGTGGCCAC AGGCCGTGAC ATCATTGACA AGCAGCACAC AGAACAAGAG GCCAGCTACG 1081 GCAGAGACGC CTTTGCCAAG GCAATATATG AGCGCCTTTT TTGTTGGATC GTTACTCGCA 1141 TCAATGATAT TATTGAGGTC AAGAACTATG ACACCACAAT CCATGGGAAA AACACTGTTA 1201 TTGGTGTCTT GGATATCTAT GGCTTTGAAA TCTTTGACAA CAACAGTTTT GAACAATTCT 1261 GTATCAATTA CTGCAATGAG AAACTGCAGC AGCTATTTAT TCAGCTGGTT CTGAAGCAAG 1321 AACAAGAGGA ATACCAGCGG GAAGGGATCC CCTGGAAACA TGTGGGATTG CTATACCCAA 1381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1501 TATCTTGTGG AAAGGACGAC ACGTTTAACA CTTGGCGGCC GTTACGCGTT AAGTCgacaa 1561 tcaacctctg gattacaaaa tttgtgaaag att