Construct: ORF TRCN0000480583
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017459.4_s317c1
- Derived from:
- ccsbBroadEn_06773
- DNA Barcode:
- GCTTCGTTGCTTAAGTGCGTCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKAR2B (5577)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480583
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5577 | PRKAR2B | protein kinase cAMP-depende... | NM_002736.3 | 99.8% | 99.7% | 96G>T;154G>T |
| 2 | human | 5577 | PRKAR2B | protein kinase cAMP-depende... | XM_011516398.1 | 93.1% | 93% | 96G>T;154G>T;395_396ins84 |
| 3 | human | 5577 | PRKAR2B | protein kinase cAMP-depende... | XM_024446836.1 | 61.2% | 61.2% | 0_1ins486 |
| 4 | mouse | 19088 | Prkar2b | protein kinase, cAMP depend... | NM_011158.3 | 91% | 96.8% | (many diffs) |
| 5 | mouse | 19088 | Prkar2b | protein kinase, cAMP depend... | XM_006515014.3 | 91% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1320
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag catcgagatc ccggcgggac tgacggagct gctgcagggc ttcacggtgg 121 aggtgctgag gcaccagccc gcggacctgc tggagttcgc tctgcagcac ttcacccgcc 181 tgcagcagga gaacgagcgc aaaggcaccg cgcgcttctg ccatgagggc aggacctggg 241 gggacctggg cgccgctgcc gggggcggca cccccagcaa gggggtcaac ttcgccgagg 301 agcccatgca gtccgactcc gaggacgggg aggaggagga ggcggcgccc gcggacgcag 361 gggcgttcaa tgctccagta ataaaccgat tcacaaggcg tgcctcagta tgtgcagaag 421 cttataatcc tgatgaagaa gaagatgatg cagagtccag gattatacat ccaaaaactg 481 atgatcaaag aaataggttg caagaggctt gcaaagacat cctgctgttt aagaatctgg 541 atccggagca gatgtctcaa gtattagatg ccatgtttga aaaattggtc aaagatgggg 601 agcatgtaat tgatcaaggt gacgatggtg acaactttta tgtaattgat agaggcacat 661 ttgatattta tgtgaaatgt gatggtgttg gaagatgtgt tggtaactat gataatcgtg 721 ggagtttcgg cgaactggcc ttaatgtaca atacacccag agcagctaca atcactgcta 781 cctctcctgg tgctctgtgg ggtttggaca gggtaacctt caggagaata attgtgaaaa 841 acaatgccaa aaagagaaaa atgtatgaaa gctttattga gtcactgcca ttccttaaat 901 ctttggagtt ttctgaacgc ctgaaagtag tagatgtgat aggcaccaaa gtatacaacg 961 atggagaaca aatcattgcT CAGGGAGATT CGGCTGATTC TTTTTTCATT GTAGAATCTG 1021 GAGAAGTGAA AATTACTATG AAAAGAAAGG GTAAATCAGA AGTGGAAGAG AATGGTGCAG 1081 TAGAAATCGC TCGATGCTCG CGGGGACAGT ACTTTGGAGA GCTTGCCCTG GTAACTAACA 1141 AACCTCGAGC AGCTTCTGCC CACGCCATTG GGACTGTCAA ATGTTTAGCA ATGGATGTGC 1201 AAGCATTTGA AAGGCTTCTG GGACCTTGCA TGGAAATTAT GAAAAGGAAC ATCGCTACCT 1261 ATGAAGAACA GTTAGTTGCC CTGTTTGGAA CGAACATGGA TATTGTTGAA CCCACTGCAT 1321 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAATTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGAGCTTC GTTGCTTAAG TGCGTCTTGA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt