Construct: ORF TRCN0000480628
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014511.1_s317c1
- Derived from:
- ccsbBroadEn_08696
- DNA Barcode:
- TGCGGCATATCCTATAACCGCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPA6 (57094)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480628
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57094 | CPA6 | carboxypeptidase A6 | NM_020361.5 | 99.9% | 99.7% | 518C>G |
| 2 | human | 57094 | CPA6 | carboxypeptidase A6 | XM_011517570.2 | 66% | 65.9% | 0_1ins444;74C>G |
| 3 | human | 57094 | CPA6 | carboxypeptidase A6 | XM_017013646.1 | 66% | 65.9% | 0_1ins444;74C>G |
| 4 | human | 57094 | CPA6 | carboxypeptidase A6 | XM_017013647.1 | 52.3% | 48.6% | (many diffs) |
| 5 | human | 57094 | CPA6 | carboxypeptidase A6 | XR_001745565.1 | 47.7% | (many diffs) | |
| 6 | mouse | 329093 | Cpa6 | carboxypeptidase A6 | NM_177834.4 | 86.2% | 84.2% | (many diffs) |
| 7 | mouse | 329093 | Cpa6 | carboxypeptidase A6 | NM_001289497.1 | 52.2% | 54.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1377
- ORF length:
- 1311
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gtgtctcggg aagcgcaggg gccaggcagc tgctttcctg cctctttgct 121 ggctcttttt gaagattctg caaccggggc acagccacct ttataacaac cgctatgctg 181 gtgataaagt gataagattt attcccaaaa cagaagagga agcatatgca ctgaagaaaa 241 tatcctatca acttaaggtg gacctgtggc agcccagcag tatctcctat gtatcagagg 301 gaacagttac tgatgtccat atcccccaaa atggttcccg agccctgtta gccttcttac 361 aggaagccaa catccagtac aaggtcctca tagaagatct tcagaaaaca ctggagaagg 421 gaagcagctt gcacacccag agaaaccgaa gatccctctc tggatataat tatgaagttt 481 atcactcctt agaagaaatt caaaattgga tgcatcatct gaataaaact cactcaggcc 541 tcattcacat gttctctatt ggaagatcat atgagggaag atgtcttttt attttaaagc 601 tgggcagacg atcacgactc aaaagagctg tttggataga ctgtggtatt catgcaagag 661 aatggattgg tcctgccttt tgtcagtggt ttgtaaaaga agctcttcta acatataaga 721 gtgacccagc catgagaaaa atgttgaatc atctatattt ctatatcatg cctgtgttta 781 acgtcgatgg ataccatttt agttggacca atgatcgatt ttggagaaaa acaaggtcaa 841 ggaactcaag gtttcgctgc cgtggagtgg atgccaatag aaactggaaa gtgaagtggt 901 gtgatgaagg agcttctatg cacccttgtg atgacacata ctgtggccct tttccagaat 961 ctgagccgga agtgaaggct gtagctaact tccttcGAAA ACACAGAAAG CACATTAGGG 1021 CTTATCTCTC CTTTCATGCA TATGCTCAGA TGTTACTGTA TCCCTATTCT TACAAATATG 1081 CAACAATTCC CAATTTTAGA TGTGTGGAAT CTGCAGCTTA TAAAGCTGTG AATGCACTTC 1141 AGTCAGTATA CGGGGTACGA TACAGATATG GACCAGCCTC CACAACGTTG TATGTGAGCT 1201 CTGGTAGCTC AATGGATTGG GCCTACAAAA ATGGAATACC TTATGCATTT GCTTTCGAAC 1261 TACGTGACAC TGGATATTTT GGATTTTTAC TCCCAGAGAT GCTCATCAAA CCCACCTGTA 1321 CAGAAACTAT GCTGGCTGTG AAAAATATCA CAATGCACCT GCTAAAGAAA TGTCCCTGCC 1381 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1441 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1501 ATATATCTTG TGGAAAGGAC GATGCGGCAT ATCCTATAAC CGCCCTACGC GTTAAGTCga 1561 caatcaacct ctggattaca aaatttgtga aagatt