Construct: ORF TRCN0000480629
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013748.1_s317c1
- Derived from:
- ccsbBroadEn_04474
- DNA Barcode:
- TACACATACCCGTACACGGAGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRTAP4-2 (85291)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480629
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85291 | KRTAP4-2 | keratin associated protein 4-2 | NM_033062.4 | 99.7% | 99.2% | 284A>G |
| 2 | human | 100132476 | KRTAP4-7 | keratin associated protein 4-7 | NM_033061.3 | 75.2% | 70.3% | (many diffs) |
| 3 | human | 84616 | KRTAP4-4 | keratin associated protein 4-4 | NM_032524.2 | 70.4% | 69% | (many diffs) |
| 4 | human | 728224 | KRTAP4-8 | keratin associated protein 4-8 | NM_031960.3 | 66% | 57.8% | (many diffs) |
| 5 | human | 85289 | KRTAP4-5 | keratin associated protein 4-5 | NM_033188.4 | 64.2% | 59.6% | (many diffs) |
| 6 | human | 83755 | KRTAP4-12 | keratin associated protein ... | NM_031854.3 | 64% | 62.6% | (many diffs) |
| 7 | human | 653240 | KRTAP4-11 | keratin associated protein ... | NM_033059.4 | 63.1% | 56.9% | (many diffs) |
| 8 | human | 728224 | KRTAP4-8 | keratin associated protein 4-8 | NM_001322457.1 | 62.6% | 54.8% | (many diffs) |
| 9 | human | 81871 | KRTAP4-6 | keratin associated protein 4-6 | NM_030976.1 | 60% | 58.5% | (many diffs) |
| 10 | human | 100996750 | LOC100996750 | keratin-associated protein 4-7 | NM_001365252.1 | 57.3% | 52.8% | (many diffs) |
| 11 | human | 100132386 | KRTAP4-9 | keratin associated protein 4-9 | NM_001146041.1 | 56.8% | 53.8% | (many diffs) |
| 12 | mouse | 69464 | Krtap4-13 | keratin associated protein ... | NM_027087.3 | 70.5% | 66.2% | (many diffs) |
| 13 | mouse | 670482 | Gm11554 | predicted gene 11554 | NM_001099313.2 | 62.5% | 56.5% | (many diffs) |
| 14 | mouse | 670472 | Gm11569 | predicted gene 11569 | NM_001099312.1 | 62% | 55.3% | (many diffs) |
| 15 | mouse | 68768 | Krtap4-6 | keratin associated protein 4-6 | NM_026834.2 | 61.4% | 52% | (many diffs) |
| 16 | mouse | 100040248 | Gm11563 | predicted gene 11563 | NM_001126320.2 | 60.9% | 60.5% | (many diffs) |
| 17 | mouse | 670496 | Gm11564 | predicted gene 11564 | NM_001100614.1 | 60.6% | 53.4% | (many diffs) |
| 18 | mouse | 670464 | Gm11596 | predicted gene 11596 | NM_001099311.2 | 59.2% | 50% | (many diffs) |
| 19 | mouse | 76444 | Krtap4-7 | keratin associated protein 4-7 | NM_029613.1 | 56.8% | 53.2% | (many diffs) |
| 20 | mouse | 665992 | Krtap4-8 | keratin associated protein 4-8 | NM_001085547.2 | 54% | 52.3% | (many diffs) |
| 21 | mouse | 665998 | Krtap4-9 | keratin associated protein 4-9 | NM_001085548.2 | 48.9% | 44.4% | (many diffs) |
| 22 | mouse | 100040276 | Gm11595 | predicted gene 11595 | NM_001126322.1 | 41.5% | 37.5% | (many diffs) |
| 23 | mouse | 100502865 | 2300003K06Rik | RIKEN cDNA 2300003K06 gene | NM_001195383.1 | 40.6% | 32.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 477
- ORF length:
- 408
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggTCAACTCC TGTTGTGGCT CTGTGTGCTC TGACCAGGGC TGTGGCCTAG 121 AGAACTGCTG CCGTCCCAGC TGCTGCCAGA CCACCTGCTG CAGGACCACC TGCTGCCGCC 181 CCAGCTGCTG TGTGTCCAGC TGCTGCAGAC CGCAGTGCTG CCAGTCTGTG TGCTGCCAGC 241 CCACCTGCTG CAGCCCCAGC TGCTGCCAGA CCACTTGCTG CAGGACCACC TGCTGCCGTC 301 CCAGCTGCTG TGTGTCCAGC TGCTTCAGAC CCCAGTGCTG CCAGTCTGTG TGCTGCCAGC 361 CCACCTGCTG CCGCCCCAGC TGTGGCCAGA CCACCTGCTG CAGGACCACC TGCTACCGCC 421 CCAGCTGCTG TGTGTCCACC TGCTGCCGCC CAACCTGCTC TAGTGGCTCT TGCTGCTTGC 481 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 541 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 601 ATATATCTTG TGGAAAGGAC GATACACATA CCCGTACACG GAGCCCACGC GTTAAGTCga 661 caatcaacct ctggattaca aaatttgtga aagatt