Construct: ORF TRCN0000480668
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007772.2_s317c1
- Derived from:
- ccsbBroadEn_05055
- DNA Barcode:
- GGTCCCTAGTCCCCTATCTTCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAFA4 (151647)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480668
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151647 | TAFA4 | TAFA chemokine like family ... | NM_001005527.2 | 100% | 100% | |
| 2 | human | 151647 | TAFA4 | TAFA chemokine like family ... | NM_182522.5 | 100% | 100% | |
| 3 | human | 151647 | TAFA4 | TAFA chemokine like family ... | XM_011533371.1 | 77% | 72.5% | (many diffs) |
| 4 | human | 151647 | TAFA4 | TAFA chemokine like family ... | XM_011533372.1 | 77% | 72.5% | (many diffs) |
| 5 | mouse | 320701 | Fam19a4 | family with sequence simila... | NM_177233.5 | 85.2% | 90% | (many diffs) |
| 6 | mouse | 320701 | Fam19a4 | family with sequence simila... | XM_017321648.1 | 85.2% | 90% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 86
- ORF end:
- 506
- ORF length:
- 420
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctTGCA ACAAATTGAT GAGCAATGCT TTTTTATAAT 61 GCCAACTTTG TACAAAAAAG TTGGCATGAG GTCCCCAAGG ATGAGAGTCT GTGCTAAGTC 121 AGTGTTGCTG TCGCACTGGC TCTTTCTAGC CTACGTGTTA ATGGTGTGCT GTAAGCTGAT 181 GTCCGCCTCA AGCCAGCACC TCCGGGGACA TGCAGGTCAC CACCAAATCA AGCAAGGGAC 241 CTGTGAGGTG GTCGCCGTGC ACAGGTGCTG CAATAAGAAC CGCATAGAAG AGCGGTCACA 301 AACGGTCAAG TGCTCTTGCT TCCCGGGACA GGTGGCGGGC ACAACTCGGG CTCAACCTTC 361 TTGTGTTGAA GCTTCCATTG TGATTCAGAA ATGGTGGTGT CACATGAATC CGTGTTTGGA 421 AGGAGAGGAT TGTAAAGTGC TGCCAGATTA CTCAGGTTGG TCCTGTAGCA GTGGCAATAA 481 AGTCAAAACT ACGAAGGTAA CGCGGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG 541 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 601 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGTCCCTAG 661 TCCCCTATCT TCAAGACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 721 agatt