Construct: ORF TRCN0000480737
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003745.1_s317c1
- Derived from:
- ccsbBroadEn_06172
- DNA Barcode:
- CCTAAATCACCCTATCTGTTGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPS8 (2059)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480737
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2059 | EPS8 | epidermal growth factor rec... | NM_004447.6 | 99.9% | 99.8% | 1892C>T |
| 2 | human | 2059 | EPS8 | epidermal growth factor rec... | XM_024448879.1 | 99.9% | 99.8% | 1892C>T |
| 3 | human | 2059 | EPS8 | epidermal growth factor rec... | XM_024448880.1 | 99.9% | 99.8% | 1892C>T |
| 4 | human | 2059 | EPS8 | epidermal growth factor rec... | XM_024448881.1 | 99.9% | 99.8% | 1892C>T |
| 5 | human | 2059 | EPS8 | epidermal growth factor rec... | XM_024448882.1 | 99.9% | 99.8% | 1892C>T |
| 6 | human | 2059 | EPS8 | epidermal growth factor rec... | XM_024448878.1 | 98.6% | 98.5% | 1_33del;1925C>T |
| 7 | human | 2059 | EPS8 | epidermal growth factor rec... | XM_011520605.3 | 97.5% | 97.5% | 1_60del;1952C>T |
| 8 | mouse | 13860 | Eps8 | epidermal growth factor rec... | NM_001271588.1 | 84.4% | 88.9% | (many diffs) |
| 9 | mouse | 13860 | Eps8 | epidermal growth factor rec... | NM_001271595.1 | 84.4% | 88.9% | (many diffs) |
| 10 | mouse | 13860 | Eps8 | epidermal growth factor rec... | NM_007945.3 | 84.4% | 88.9% | (many diffs) |
| 11 | mouse | 13860 | Eps8 | epidermal growth factor rec... | XM_011241204.2 | 84.4% | 88.9% | (many diffs) |
| 12 | mouse | 13860 | Eps8 | epidermal growth factor rec... | XM_017321383.1 | 83% | 86.9% | (many diffs) |
| 13 | mouse | 13860 | Eps8 | epidermal growth factor rec... | NM_001271587.1 | 81.8% | 86.2% | (many diffs) |
| 14 | mouse | 13860 | Eps8 | epidermal growth factor rec... | XM_006505508.3 | 81.5% | 86.3% | (many diffs) |
| 15 | mouse | 13860 | Eps8 | epidermal growth factor rec... | NM_001271589.1 | 57.2% | 60.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2532
- ORF length:
- 2466
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tggtcatatt tctaatcatc ccagtagttt tggaatgtac ccatctcaga 121 tgaatggcta cggatcatca cctacctttt cccagacgga cagagaacat ggttcaaaaa 181 caagtgcaaa ggccctttat gaacaaagga agaattatgc acgggacagt gtcagcagtg 241 tgtcagatat atctcaatac cgtgttgaac acttgactac ctttgtcctg gatcggaaag 301 atgctatgat cactgttgat gatggaataa ggaaattgaa attgcttgat gccaagggca 361 aagtgtggac tcaagatatg attcttcaag tggatgacag agctgtgagc ctgattgatt 421 tagaatcaaa gaatgaactg gagaattttc ctttaaacac aatccagcac tgccaagctg 481 tgatgcattc atgcagctat gattcagttc ttgcactggt gtgcaaagag ccaacccaga 541 acaagccaga tcttcatctc ttccagtgtg atgaggttaa ggcaaaccta attagtgaag 601 atattgaaag tgcaatcagt gacagtaaag gagggaaaca gaagaggcgg cccgacgccc 661 tgaggatgat ttccaatgca gaccctagta taccgcctcc acccagagct cctgcccctg 721 cgccccctgg gaccgtcacc caggtggatg ttagaagtcg agtggcagcc tggtctgcat 781 gggcagccga ccaaggggac tttgagaaac caaggcagta tcatgagcag gaagaaacac 841 ctgagatgat ggcagcccgc attgacagag atgtgcaaat cttaaaccac attttggatg 901 acattgaatt ttttatcaca aaactccaaa aagcagcaga agcattttct gagctttcta 961 aaaggaagaa aaacaagaaa ggtaaaagga aaggaccagg agagggtgtt ttaacgctgc 1021 gggcaaaacc tccacctcct gatgaatttc ttgactgttt ccaaaagttt aaacacggat 1081 ttaaccttct ggccaaactg aagtctcata ttcagaatcc tagtgctgca gatttggttc 1141 actttttgtt tactccatta aatatggtgg tgcaggcaac aggaggtcct gaactagcca 1201 gttcagtact tagtccccta ttgaataagg acacaattga tttcttaaat tatactgtca 1261 atggtgatga acggcagctg tggatgtcat tgggaggaac ttggatgaaa gccagagcag 1321 agtggccaaa agaacagttt attccaccat atgttccacg attccgcaat ggctgggagc 1381 ccccaatgct gaactttatg ggagccacaa tggaacaaga tctttatcaa ctggcagaat 1441 ctgtggcaaa tgtagcagaa catcagcgca aacaggaaat aaaaagatta tccacagagc 1501 attccagtgt atcagagtat catccagccg atggctatgc gttcagtagc aacatttaca 1561 caagaggatc ccacctggac caaggggaag ctgctgttgc ttttaagcca acttctaatc 1621 gccatataga tagaaattat gaaccactca aaacacaacc caagaaatat gccaaatcca 1681 agtatgactt tgtagcaagg aacaacagtg agctctcggt tctaaaggat gatattttag 1741 agatacttga tgatcggaag caatggtgga aagttcgaaa tgcaagtgga gactctggat 1801 ttgtgccaaa taacattttg gatattgtga gacctccaga atctggattg gggcgtgctg 1861 atccacctta tactcatact atacagaaac aaaggatgga gtatggccca agaccagctg 1921 atactccccc tgctccatca cctcctccaa caccagttcc tgttcctgtt ccccttcccc 1981 cttccactcc agcacctgtt cctgtgtcaa aggtcccagc aaatataaca cgtcaaaaca 2041 gcagctccag tgacagtggt ggcagtatcg tgcgagacag ccagagacac aaacaacttc 2101 cggtggaccg aaggaaatcT CAGATGGAGG AAGTGCAAGA TGAACTCATC CACAGACTGA 2161 CCATTGGTCG GAGTGCCGCT CAGAAGAAAT TCCATGTGCC ACGGCAGAAC GTGCCAGTTA 2221 TCAATATCAC TTACGACTCC ACACCAGAGG ATGTGAAGAC GTGGTTACAG TCAAAGGGAT 2281 TCAACCCTGT GACTGTCAAT AGTCTTGGAG TATTAAATGG TGCACAACTT TTCTCTCTCA 2341 ATAAGGATGA ACTGAGGACA GTCTGCCCTG AAGGGGCGAG AGTCTATAGC CAAATCACTG 2401 TACAAAAAGC TGCATTGGAG GATAGCAGTG GCAGCTCCGA GTTACAAGAA ATTATGAGAA 2461 GACGACAGGA AAAAATCAGT GCTGCCGCTA GTGATTCAGG AGTGGAATCT TTTGATGAAG 2521 GAAGCAGTCA CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2581 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2641 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCT AAATCACCCT ATCTGTTGCG 2701 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t