Construct: ORF TRCN0000480742
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001433.1_s317c1
- Derived from:
- ccsbBroadEn_01668
- DNA Barcode:
- CATGCGGCTCTGCCTTACAATGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- THPO (7066)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480742
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7066 | THPO | thrombopoietin | NM_001177597.2 | 100% | 100% | |
| 2 | human | 7066 | THPO | thrombopoietin | NM_001290022.1 | 100% | 100% | |
| 3 | human | 7066 | THPO | thrombopoietin | NM_000460.4 | 98.8% | 98.8% | 394_405del |
| 4 | human | 7066 | THPO | thrombopoietin | NM_001289998.1 | 98.8% | 98.8% | 394_405del |
| 5 | human | 7066 | THPO | thrombopoietin | NM_001290028.1 | 98.8% | 98.8% | 394_405del |
| 6 | human | 7066 | THPO | thrombopoietin | NM_001177598.2 | 91.4% | 43% | 393_394insCAGGG;957_958ins85 |
| 7 | human | 7066 | THPO | thrombopoietin | NM_001290026.1 | 91.4% | 43% | 393_394insCAGGG;957_958ins85 |
| 8 | human | 7066 | THPO | thrombopoietin | NM_001289997.1 | 79.8% | 50.9% | 394_405del;475_476ins116;858_859ins85 |
| 9 | human | 7066 | THPO | thrombopoietin | NM_001290027.1 | 79.8% | 50.9% | 394_405del;475_476ins116;858_859ins85 |
| 10 | human | 7066 | THPO | thrombopoietin | NM_001290003.1 | 70.7% | 70.7% | 1_420del;814_825del |
| 11 | human | 7066 | THPO | thrombopoietin | XM_017007107.1 | 57.6% | 37.4% | 1_420del;883_884ins116;1266_1267ins85 |
| 12 | mouse | 21832 | Thpo | thrombopoietin | XM_017316933.1 | 23.1% | 23% | (many diffs) |
| 13 | mouse | 21832 | Thpo | thrombopoietin | XM_017316932.1 | 22.4% | 21.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1116
- ORF length:
- 1047
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagctgact gaattgctcc tcgtggtcat gcttctccta actgcaaggc 121 taacgctgtc cagcccggct cctcctgctt gtgacctccg agtcctcagt aaactgcttc 181 gtgactccca tgtccttcac agcagactga gccagtgccc agaggttcac cctttgccta 241 cacctgtcct gctgcctgct gtggacttta gcttgggaga atggaaaacc cagatggagg 301 agaccaaggc acaggacatt ctgggagcag tgacccttct gctggaggga gtgatggcag 361 cacggggaca actgggaccc acttgcctct catccctcct ggggcagctt tctggacagg 421 tccgtctcct ccttggggcc ctgcagagcc tccttggaac ccagggcagg accacagctc 481 acaaggatcc caatgccatc ttcctgagct tccaacacct gctccgagga aaggtgcgtt 541 tcctgatgct tgtaggaggg tccaccctct gcgtcaggcg ggccccaccc accacagctg 601 tccccagcag aacctctcta gtcctcacac tgaacgagct cccaaacagg acttctggat 661 tgttggagac aaacttcact gcctcagcca gaactactgg ctctgggctt ctgaagtggc 721 agcagggatt cagagccaag attcctggTC TGCTGAACCA AACCTCCAGG TCCCTGGACC 781 AAATCCCCGG ATACCTGAAC AGGATACACG AACTCTTGAA TGGAACTCGT GGACTCTTTC 841 CTGGACCCTC ACGCAGGACC CTAGGAGCCC CGGACATTTC CTCAGGAACA TCAGACACAG 901 GCTCCCTGCC ACCCAACCTC CAGCCTGGAT ATTCTCCTTC CCCAACCCAT CCTCCTACTG 961 GACAGTATAC GCTCTTCCCT CTTCCACCCA CCTTGCCCAC CCCTGTGGTC CAGCTCCACC 1021 CCCTGCTTCC TGACCCTTCT GCTCCAACGC CCACCCCTAC CAGCCCTCTT CTAAACACAT 1081 CCTACACCCA CTCCCAGAAT CTGTCTCAGG AAGGGTTGCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 ACATGCGGCT CTGCCTTACA ATGCGACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt