Construct: ORF TRCN0000480760
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012722.1_s317c1
- Derived from:
- ccsbBroadEn_07541
- DNA Barcode:
- TCTAATCCCGCACTTGCATAGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FARSB (10056)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480760
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10056 | FARSB | phenylalanyl-tRNA synthetas... | NM_005687.5 | 99.8% | 99.8% | 1653C>T;1753G>A |
| 2 | human | 10056 | FARSB | phenylalanyl-tRNA synthetas... | XM_006712169.2 | 83% | 83% | 0_1ins297;1356C>T;1456G>A |
| 3 | human | 10056 | FARSB | phenylalanyl-tRNA synthetas... | XM_011510466.2 | 83% | 83% | 0_1ins297;1356C>T;1456G>A |
| 4 | human | 10056 | FARSB | phenylalanyl-tRNA synthetas... | XM_017003110.2 | 83% | 83% | 0_1ins297;1356C>T;1456G>A |
| 5 | human | 10056 | FARSB | phenylalanyl-tRNA synthetas... | XM_024452492.1 | 83% | 83% | 0_1ins297;1356C>T;1456G>A |
| 6 | human | 10056 | FARSB | phenylalanyl-tRNA synthetas... | NR_130154.2 | 25.3% | (many diffs) | |
| 7 | mouse | 23874 | Farsb | phenylalanyl-tRNA synthetas... | NM_001278075.1 | 87.3% | 93.7% | (many diffs) |
| 8 | mouse | 23874 | Farsb | phenylalanyl-tRNA synthetas... | NM_011811.4 | 87.3% | 93.7% | (many diffs) |
| 9 | mouse | 23874 | Farsb | phenylalanyl-tRNA synthetas... | XR_865550.1 | 77.3% | (many diffs) | |
| 10 | mouse | 23874 | Farsb | phenylalanyl-tRNA synthetas... | NR_102758.1 | 65.6% | (many diffs) | |
| 11 | mouse | 23874 | Farsb | phenylalanyl-tRNA synthetas... | NR_102757.1 | 64.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1833
- ORF length:
- 1767
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gactgtcagc gtgaagcgtg atctgctctt ccaagccctg ggccgcacct 121 acactgacga agaatttgat gaactatgtt ttgaatttgg tctggagctt gatgaaatta 181 catctgagaa ggaaataata agtaaagaac aaggtaatgt aaaggcagca ggagcctctg 241 atgttgttct ttacaaaatt gacgtccctg ccaatagata tgatctcctg tgtctggaag 301 gattggttcg aggacttcag gtcttcaaag aaaggataaa ggctccagtg tataaacggg 361 taatgcctga tggaaaaatc cagaaattga ttatcacaga agagacagct aagatacgtc 421 cttttgcggt agcagcagtt ctccgtaata taaagtttac taaagatcga tatgacagct 481 tcattgaact tcaggagaaa ttacatcaga atatttgcag gaaaagagca ctggttgcca 541 ttggtaccca tgatttggac actttgtcgg gcccatttac ttatactgca aagcgtcctt 601 cagatatcaa attcaagcct ctaaataaga ccaaggagta tacagcctgt gaactgatga 661 acatatacaa gactgacaat cacctgaaac attatttaca tatcattgaa aacaaacccc 721 tgtatccagt tatctatgat agcaatggtg tcgtcctttc aatgcctccc atcatcaatg 781 gggatcattc cagaataaca gtaaatacta gaaatatttt tattgaatgc acgggaactg 841 actttactaa ggcaaaaata gttcttgata ttattgtcac catgttcagt gaatattgtg 901 agaatcaatt tacggtcgaa gctgctgaag tggtttttcc taatggaaaa tcacatacct 961 ttccagaatt agcttaccga aaggagatgg tgagagctga cctaattaac aaaaaagttg 1021 gaatcagaga aactccagaa aatcttgcca aacttctgac caggatgtat ttaaaatcag 1081 aagtcatagg tgatgggaat cagattgaga ttgaaatccc tccaaccaga gctgacatta 1141 tccatgcatg tgatattgta gaagatgcag ctattgctta tggatataac aacattcaga 1201 tgactctccc gaaaacttac accatagcta atcaatttcc tcttaataag ctcactgaac 1261 ttctccgaca tgacatggca gccgctggct tcactgaagc acttaccttt gccctgtgct 1321 cccaagaaga tattgctgat aaactaggtg tggatatctc tgcaacaaag gcagtccaca 1381 taagtaatcc taaaacagct gaatttcagg tggcacgcac tacccttctt cctggcctcc 1441 TGAAGACCAT AGCAGCAAAT CGTAAGATGC CCCTTCCACT GAAACTGTTT GAAATCTCTG 1501 ACATTGTAAT AAAAGATTCT AATACAGATG TAGGTGCAAA AAACTACAGA CATCTCTGTG 1561 CTGTTTATTA CAACAAGAAT CCTGGGTTTG AGATCATTCA TGGGCTGCTG GACAGAATTA 1621 TGCAGTTGCT CGATGTGCCT CCTGGTGAAG ACAAGGGGGG ATATGTGATC AAAGCATCAG 1681 AAGGGCCTGC TTTCTTCCCC GGGCGATGTG CAGAGATTTT TGCCAGGGGT CAAAGCGTCG 1741 GGAAGCTTGG GGTCCTTCAT CCTGACGTTA TCACCAAATT TGAGCTGACC ATGCCCTGCT 1801 CCTCCCTAGA AATCAATATT GGACCCTTTT TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1861 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1921 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 1981 TAATCCCGCA CTTGCATAGA TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 2041 ttgtgaaaga tt