Construct: ORF TRCN0000480771

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009224.1_s317c1
Derived from:
ccsbBroadEn_11685
DNA Barcode:
GCCTGCGAACGCAGCACTCCAGTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DCUN1D4 (23142)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480771
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23142 DCUN1D4 defective in cullin neddyla... XM_024453945.1 100% 100%
2 human 23142 DCUN1D4 defective in cullin neddyla... NM_001287757.1 84.9% 84.9% 435_539del
3 human 23142 DCUN1D4 defective in cullin neddyla... XM_011534380.1 84.9% 84.9% 435_539del
4 human 23142 DCUN1D4 defective in cullin neddyla... XM_024453941.1 84.9% 84.9% 435_539del
5 human 23142 DCUN1D4 defective in cullin neddyla... XM_024453942.1 84.9% 84.9% 435_539del
6 human 23142 DCUN1D4 defective in cullin neddyla... XM_024453943.1 84.9% 84.9% 435_539del
7 human 23142 DCUN1D4 defective in cullin neddyla... XM_024453944.1 84.9% 84.9% 435_539del
8 human 23142 DCUN1D4 defective in cullin neddyla... NM_015115.3 76.6% 76.6% 1_180del
9 human 23142 DCUN1D4 defective in cullin neddyla... XM_017007912.2 76% 76% 1_186del
10 human 23142 DCUN1D4 defective in cullin neddyla... NM_001040402.3 67.4% 67.4% 1_180del;615_719del
11 human 23142 DCUN1D4 defective in cullin neddyla... XM_011534379.2 67% 67% 1_186del;621_725del
12 human 23142 DCUN1D4 defective in cullin neddyla... XM_005265731.3 65.4% 65.4% 1_312del
13 human 23142 DCUN1D4 defective in cullin neddyla... NM_001287755.1 58.6% 58.6% 1_312del;747_851del
14 human 23142 DCUN1D4 defective in cullin neddyla... XM_017007913.2 50.4% 50.4% 0_1ins240;195_299del
15 human 23142 DCUN1D4 defective in cullin neddyla... XR_001741177.2 13.7% 1_291del;726_886del;1044_4299del
16 human 23142 DCUN1D4 defective in cullin neddyla... XR_001741180.2 13.7% 1_291del;726_890del;1048_4303del
17 human 23142 DCUN1D4 defective in cullin neddyla... XR_245254.3 13.6% 1_313del;748_908del;1066_4321del
18 human 23142 DCUN1D4 defective in cullin neddyla... XR_001741176.1 13.6% 1_313del;748_912del;1070_4325del
19 human 23142 DCUN1D4 defective in cullin neddyla... XR_001741179.2 13.6% 1_291del;726_917del;1075_4330del
20 human 23142 DCUN1D4 defective in cullin neddyla... XR_427521.3 13.5% 1_313del;748_939del;1097_4352del
21 human 23142 DCUN1D4 defective in cullin neddyla... XR_941046.2 13.4% 1_313del;748_984del;1142_4397del
22 human 23142 DCUN1D4 defective in cullin neddyla... XR_001741178.2 12.8% 1_291del;726_1181del;1339_4594del
23 human 23142 DCUN1D4 defective in cullin neddyla... XR_001741175.1 12.8% 1_313del;748_1203del;1361_4616del
24 mouse 100737 Dcun1d4 DCN1, defective in cullin n... XM_006503627.3 77% 81.8% (many diffs)
25 mouse 100737 Dcun1d4 DCN1, defective in cullin n... XM_006503628.3 77% 81.8% (many diffs)
26 mouse 100737 Dcun1d4 DCN1, defective in cullin n... XM_006503629.2 77% 81.8% (many diffs)
27 mouse 100737 Dcun1d4 DCN1, defective in cullin n... XM_006503630.3 77% 81.8% (many diffs)
28 mouse 100737 Dcun1d4 DCN1, defective in cullin n... XM_006503631.2 77% 81.8% (many diffs)
29 mouse 100737 Dcun1d4 DCN1, defective in cullin n... XM_006503633.3 77% 81.8% (many diffs)
30 mouse 100737 Dcun1d4 DCN1, defective in cullin n... XM_006503634.3 77% 81.8% (many diffs)
31 mouse 100737 Dcun1d4 DCN1, defective in cullin n... XM_006503636.3 77% 81.8% (many diffs)
32 mouse 100737 Dcun1d4 DCN1, defective in cullin n... XM_006503637.3 77% 81.8% (many diffs)
33 mouse 100737 Dcun1d4 DCN1, defective in cullin n... XM_006503638.2 77% 81.8% (many diffs)
34 mouse 100737 Dcun1d4 DCN1, defective in cullin n... NM_001190733.1 69.5% 73.9% (many diffs)
35 mouse 100737 Dcun1d4 DCN1, defective in cullin n... XM_017320580.1 68.7% 71.9% (many diffs)
36 mouse 100737 Dcun1d4 DCN1, defective in cullin n... XM_006503626.2 65.9% 70.1% (many diffs)
37 mouse 100737 Dcun1d4 DCN1, defective in cullin n... NM_178896.5 61.1% 65% (many diffs)
38 mouse 100737 Dcun1d4 DCN1, defective in cullin n... NM_001190734.1 58.3% 62% (many diffs)
39 mouse 100737 Dcun1d4 DCN1, defective in cullin n... XR_376792.2 12.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
657
ORF length:
591
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc accaaggaaa aagagaagac ctgcctctgg agatgattta tctgccaaga
121aaagtagaca tgatagcatg tatagaaaat atgattcgac tagaataaag actgaagaag
181aagccttttc aagtaaaagg tgcttggaat ggttctatga atatgcagga actgatgatg
241ttgtaggccc tgaaggcatg gagaaatttt gtgaagacat tggtgttgaa ccagaaaacg
301tagttatgct tgtcctagct tggaaattGG ATGCACAAAA CATGGGTTAT TTTACTCTAC
361AGGAGTGGTT AAAAGGAATG ACTTCTCTCC AATGTGATAC AACAGAAAAA CTCAGAAATA
421CTTTGGATTA CTTAAGATCA TTCTTAAATG ATTCTACAAA CTTTAAACTT ATTTACAGAT
481ATGCGTTTGA CTTTGCACGG CAATCAAAAT ACAAAGTTAT TAATAAAGAC CAGTGGTGCA
541ATGTCCTAGA GTTTAGCAGA ACAATTAATC TTGACCTCAG CAACTATGAT GAAGATGGAG
601CATGGCCAGT TTTGTTGGAC GAGTTTGTGG AGTGGTATAA AGACAAACAG ATGTCCTACC
661CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
721TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
781ATATATCTTG TGGAAAGGAC GAGCCTGCGA ACGCAGCACT CCAGTTACGC GTTAAGTCga
841caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)