Transcript: Mouse NM_001190734.1

Mus musculus DCN1, defective in cullin neddylation 1, domain containing 4 (S. cerevisiae) (Dcun1d4), transcript variant A, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Dcun1d4 (100737)
Length:
4078
CDS:
35..955

Additional Resources:

NCBI RefSeq record:
NM_001190734.1
NBCI Gene record:
Dcun1d4 (100737)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001190734.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000197683 CATACCCTTAACAAGCTGAAT pLKO.1 155 CDS 100% 4.950 6.930 N Dcun1d4 n/a
2 TRCN0000182051 CCGCTGTTTCACAGTTCCTTT pLKO.1 2467 3UTR 100% 4.950 6.930 N Dcun1d4 n/a
3 TRCN0000149004 GCTTGGAAATTGGATGCACAA pLKO.1 509 CDS 100% 4.050 5.670 N DCUN1D4 n/a
4 TRCN0000178545 GCTCATTTACAGATACGCGTT pLKO.1 658 CDS 100% 2.160 3.024 N Dcun1d4 n/a
5 TRCN0000198562 GCAGGCATGACAGCATGTATA pLKO.1 315 CDS 100% 13.200 9.240 N Dcun1d4 n/a
6 TRCN0000197956 GACGAATTTGTGGAATGGTAT pLKO.1 914 CDS 100% 4.950 3.465 N Dcun1d4 n/a
7 TRCN0000181717 GCAGTTCTTCGGACTGCTTTA pLKO.1 228 CDS 100% 1.080 0.756 N Dcun1d4 n/a
8 TRCN0000176492 CTTCCCTACAATGTGATACAA pLKO.1 573 CDS 100% 5.625 3.375 N Dcun1d4 n/a
9 TRCN0000149752 GACATTGGTGTTGAACCAGAA pLKO.1 467 CDS 100% 4.050 2.430 N DCUN1D4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001190734.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11685 pDONR223 100% 58.3% 62% None (many diffs) n/a
2 ccsbBroad304_11685 pLX_304 0% 58.3% 62% V5 (many diffs) n/a
3 TRCN0000480771 GCCTGCGAACGCAGCACTCCAGTT pLX_317 55.4% 58.3% 62% V5 (many diffs) n/a
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