Construct: ORF TRCN0000480793
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017418.1_s317c1
- Derived from:
- ccsbBroadEn_08060
- DNA Barcode:
- AGGTGCGAATACAACTGGACTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPNE7 (27132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480793
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27132 | CPNE7 | copine 7 | NM_153636.3 | 99.9% | 100% | 1638T>C |
| 2 | human | 27132 | CPNE7 | copine 7 | XM_011523000.1 | 93.7% | 88.9% | 1538_1631del;1732T>C;1769_1785del |
| 3 | human | 27132 | CPNE7 | copine 7 | XM_017023140.2 | 93% | 88.3% | (many diffs) |
| 4 | human | 27132 | CPNE7 | copine 7 | NM_014427.5 | 88% | 88.1% | 353_577del;1863T>C |
| 5 | human | 27132 | CPNE7 | copine 7 | XM_017023139.2 | 87.5% | 87.5% | 170_181del;365_589del;1875T>C |
| 6 | human | 27132 | CPNE7 | copine 7 | XM_017023138.2 | 83.2% | 79% | (many diffs) |
| 7 | human | 27132 | CPNE7 | copine 7 | XM_011523001.3 | 74% | 66.7% | (many diffs) |
| 8 | human | 27132 | CPNE7 | copine 7 | XM_017023141.1 | 51.5% | 46.9% | (many diffs) |
| 9 | mouse | 102278 | Cpne7 | copine VII | NM_170684.2 | 85.1% | 89.6% | (many diffs) |
| 10 | mouse | 102278 | Cpne7 | copine VII | XM_006530549.2 | 66.2% | 70.3% | (many diffs) |
| 11 | mouse | 102278 | Cpne7 | copine VII | XR_001778387.1 | 65.1% | (many diffs) | |
| 12 | mouse | 102278 | Cpne7 | copine VII | XR_387726.3 | 57.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1740
- ORF length:
- 1674
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cgcgggctcg gagcgcgggg cggcggcaac ccccgggggt ttgcccgcgc 121 cctgcgcctc gaaggtggag ctgcggctca gctgccggca cctgctggac cgcgacccgc 181 tcaccaagtc cgaccccagc gtggcgttgc tgcagcaggc gcagggccag tgggtgcagg 241 tgggcagaac cgaggtggtc cggagcagcc tgcatcccgt gttctccaag gtcttcacgg 301 tggactacta cttcgaggag gtgcagaggc tgcgctttga ggtgtacgac acgcatgggc 361 ccagcggctt cagctgtcag gaggacgatt tcctgggggg catggagtgc accctggggc 421 agattgtggc ccagaagaag gtgacccgcc cgctgctgct caagtttggc aggaacgctg 481 gcaagtccac catcacggtg atcgccgagg acatctcggg gaacaacggc tacgtggagc 541 tctccttccg ggccaggaag ctggacgaca aggacctctt cagcaagtcc gaccccttcc 601 tggagctcta cagggtcaac gacgaccagg gcttgcagct ggtgtacagg acggaggtgg 661 tgaagaacaa cctgaacccg gtgtgggagg ccttcaaagt ctctctgagt tccctctgca 721 gctgcgagga gacaaggcct ctaaagtgcc tggtctggga ttacgactct cgaggaaagc 781 acgacttcat cggagaattc tctaccacct tcgaggagat gcagaaggcc tttgaggagg 841 ggcaggccca gtgggactgt gtgaacccca aatacaagca gaagagacgc agttataaga 901 actcaggagt ggtcgtcctg gctgacctca agttccacag ggtgtactcc ttcctggact 961 atatcatggg cggctgccag atccacttca ccgtggccat tgacttcacc gcctccaatg 1021 gagacccgcg gaacagctgc tccctgcact acatcaaccc ctaccagccg aacgagtacc 1081 tgaaggcact ggtgtccgtg ggcgagatct gccaggacta tgacagtgac aagaggtttt 1141 ccgctttggg gtttggagcc cggatccctc ccaagtatga ggtgtcccat gactttgcca 1201 tcaatttcaa ccctgaggac gatgagtgtg aaggcatcca gggcgtggtg gaggcctacc 1261 agaactgcct gcccagggtc cagctctacg gccccaccaa cgtggcgccc atcatctcca 1321 aggtggcacg cgtggcggcg gccgaggaga gcaccgggaa agcctctcaa tactacatcc 1381 tgctgaTCCT GACGGACGGC GTGGTGACCG ACATGGCCGA CACACGGGAG GCCATTGTGC 1441 GTGCCTCACG CCTGCCCATG TCCATCATCA TCGTGGGCGT GGGCAACGCC GACTTCACCG 1501 ACATGCAGGT CCTGGACGGC GACGACGGCG TCCTGCGCTC CCCACGGGGT GAGCCCGCGC 1561 TCCGGGACAT CGTACAGTTC GTGCCCTTCC GGGAGCTCAA GAACGCATCC CCTGCGGCGC 1621 TGGCCAAGTG CGTGCTGGCC GAGGTCCCGA AGCAGGTGGT GGAGTACTAC AGCCACAGAG 1681 GCCTGCCCCC GAGAAGCCTG GGCGTCCCTG CCGGAGAGGC CAGCCCAGGC TGCACACCGT 1741 GCTCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1801 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1861 TTTATATATC TTGTGGAAAG GACGAAGGTG CGAATACAAC TGGACTCTGA CGCGTTAAGT 1921 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt