Construct: ORF TRCN0000480798
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015101.2_s317c1
- Derived from:
- ccsbBroadEn_03687
- DNA Barcode:
- GCCCGCGGTGGGCAGTGATTACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSFL1C (55968)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480798
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55968 | NSFL1C | NSFL1 cofactor | NM_016143.4 | 100% | 100% | |
| 2 | human | 55968 | NSFL1C | NSFL1 cofactor | NM_001206736.1 | 99.4% | 99.4% | 277_282delAGGAGC |
| 3 | human | 55968 | NSFL1C | NSFL1 cofactor | XM_011529301.2 | 99.1% | 99.1% | 202_210delAGAGAAAAG |
| 4 | human | 55968 | NSFL1C | NSFL1 cofactor | XM_011529300.2 | 98.6% | 98.6% | 202_210delAGAGAAAAG;286_291delAGGAGC |
| 5 | human | 55968 | NSFL1C | NSFL1 cofactor | NM_018839.4 | 91.6% | 91.6% | 444_445ins93 |
| 6 | human | 55968 | NSFL1C | NSFL1 cofactor | XM_011529304.1 | 84.9% | 81.8% | (many diffs) |
| 7 | human | 55968 | NSFL1C | NSFL1 cofactor | XM_017027957.2 | 84.5% | 81.4% | (many diffs) |
| 8 | human | 55968 | NSFL1C | NSFL1 cofactor | XM_017027956.1 | 84.4% | 82.7% | (many diffs) |
| 9 | human | 55968 | NSFL1C | NSFL1 cofactor | XM_011529302.2 | 84% | 82.2% | (many diffs) |
| 10 | human | 55968 | NSFL1C | NSFL1 cofactor | XM_006723592.3 | 70.5% | 64.6% | 0_1ins238;41_42ins31;44_45ins58 |
| 11 | human | 55968 | NSFL1C | NSFL1 cofactor | XM_006723593.3 | 70.5% | 64.6% | 0_1ins238;41_42ins31;44_45ins58 |
| 12 | human | 55968 | NSFL1C | NSFL1 cofactor | XM_006723594.3 | 70% | 67% | 0_1ins238;38_39ins95 |
| 13 | human | 55968 | NSFL1C | NSFL1 cofactor | XM_017027958.2 | 69.9% | 67% | (many diffs) |
| 14 | human | 55968 | NSFL1C | NSFL1 cofactor | XR_001754341.2 | 36.4% | (many diffs) | |
| 15 | human | 55968 | NSFL1C | NSFL1 cofactor | XR_002958493.1 | 27.7% | (many diffs) | |
| 16 | human | 55968 | NSFL1C | NSFL1 cofactor | NR_038164.1 | 25.7% | (many diffs) | |
| 17 | mouse | 386649 | Nsfl1c | NSFL1 (p97) cofactor (p47) | NM_001291074.1 | 91.8% | 96.2% | (many diffs) |
| 18 | mouse | 386649 | Nsfl1c | NSFL1 (p97) cofactor (p47) | NM_198326.3 | 91.3% | 95.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1176
- ORF length:
- 1110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggagcga caggaggcgc tgagggagtt cgtggcggtg acgggcgccg 121 aggaggaccg ggcccgcttc tttctcgagt cggccggctg ggacttgcag atcgcgctag 181 cgagctttta tgaggacgga ggggatgaag acattgtgac catttcgcag gcaaccccca 241 gttcagtgtc cagaggcaca gcccccagtg ataatagagt gacatccttc agagacctca 301 ttcatgacca agatgaagat gaggaggaag aggaaggcca gaggttttat gctgggggct 361 cagagagaag tggacagcag attgttggcc ctcccaggaa gaaaagtccc aacgagctgg 421 tggatgatct ctttaaaggt gccaaagagc atggagctgt agctgtggag cgagtgacca 481 agagccctgg agagaccagt aaaccgagac catttgcagg aggtggctac cgccttgggg 541 cagcaccaga ggaagagtct gcctatgtgg caggagaaaa gaggcagcat tccagccaag 601 atgttcatgt agtattgaaa ctctggaaga gtggattcag cctggataat ggagaactca 661 gaagctacca agacccatcc aatgcccagt ttctggagtc tatccgcaga ggggaggtgc 721 cagcagagct tcggaggcta gcTCACGGTG GACAGGTGAA CTTGGATATG GAGGACCATC 781 GGGACGAGGA CTTTGTGAAG CCCAAAGGAG CCTTCAAAGC CTTCACTGGC GAGGGTCAGA 841 AACTGGGCAG CACTGCCCCC CAGGTGTTGA GTACCAGCTC TCCAGCCCAA CAGGCAGAAA 901 ATGAAGCCAA AGCCAGCTCT TCCATCTTAA TCGACGAATC AGAGCCTACC ACAAACATCC 961 AAATTCGGCT TGCAGACGGC GGGAGGCTGG TGCAGAAATT TAACCACAGC CACAGGATCA 1021 GCGACATCCG ACTCTTCATC GTGGATGCCC GGCCAGCCAT GGCTGCCACC AGCTTTATCC 1081 TCATGACTAC TTTCCCGAAC AAAGAGCTGG CTGATGAGAG CCAGACCCTG AAGGAAGCCA 1141 ACCTGCTCAA TGCTGTCATC GTGCAGCGGT TAACATATCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 AGCCCGCGGT GGGCAGTGAT TACAGACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt