Construct: ORF TRCN0000480825
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002522.1_s317c1
- Derived from:
- ccsbBroadEn_07666
- DNA Barcode:
- TGTTGGCTCTCGCACATGCACCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD226 (10666)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480825
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10666 | CD226 | CD226 molecule | NM_001303618.2 | 99.9% | 99.7% | 919A>G |
| 2 | human | 10666 | CD226 | CD226 molecule | NM_006566.4 | 99.9% | 99.7% | 919A>G |
| 3 | human | 10666 | CD226 | CD226 molecule | XM_005266642.3 | 99.9% | 99.7% | 919A>G |
| 4 | human | 10666 | CD226 | CD226 molecule | XM_006722374.3 | 62.8% | 61.9% | (many diffs) |
| 5 | human | 10666 | CD226 | CD226 molecule | NM_001303619.2 | 53.7% | 53.5% | 0_1ins465;454A>G |
| 6 | human | 10666 | CD226 | CD226 molecule | XM_005266643.3 | 53.7% | 53.5% | 0_1ins465;454A>G |
| 7 | human | 10666 | CD226 | CD226 molecule | XM_017025525.1 | 53.7% | 53.5% | 0_1ins465;454A>G |
| 8 | human | 10666 | CD226 | CD226 molecule | XM_017025526.1 | 53.7% | 53.5% | 0_1ins465;454A>G |
| 9 | human | 10666 | CD226 | CD226 molecule | XM_017025527.1 | 53.7% | 53.5% | 0_1ins465;454A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1077
- ORF length:
- 1008
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggattatcct actttacttt tggctcttct tcatgtatac agagctctat 121 gtgaagaggt gctttggcat acatcagttc cctttgccga gaacatgtct ctagaatgtg 181 tgtatccatc aatgggcatc ttaacacagg tggagtggtt caagatcggg acccagcagg 241 attccatagc cattttcagc cctactcatg gcatggtcat aaggaagccc tatgctgaga 301 gggtttactt tttgaattca acgatggctt ccaataacat gactcttttc tttcggaatg 361 cctctgaaga tgatgttggc tactattcct gctctcttta cacttaccca cagggaactt 421 ggcagaaggt gatacaggtg gttcagtcag atagttttga ggcagctgtg ccatcaaata 481 gccacattgt ttcggaacct ggaaagaatg tcacactcac ttgtcagcct cagatgacgt 541 ggcctgtgca ggcagtgagg tgggaaaaga tccagccccg tcagatcgac ctcttaactt 601 actgcaactt ggtccatggc agaaatttca cctccaagtt cccaagacaa atagtgagca 661 actgcagcca cggaaggtgg agcgtcatcg tcatccccga tgtcacagtc tcagactcgg 721 ggctttaccg ctgctacttg caggCCAGCG CAGGAGAAAA CGAAACCTTC GTGATGAGAT 781 TGACTGTAGC CGAGGGTAAA ACCGATAACC AATATACCCT CTTTGTGGCT GGAGGGACAG 841 TTTTATTGTT GTTGTTTGTT ATCTCAATTA CCACCATCAT TGTCATTTTC CTTAACAGAA 901 GGAGAAGGAG AGAGAGAAGA GATCTATTTA CAGAGTCCTG GGATACACAG AAGGCACCCA 961 ATAACTATAG AAGTCCCATC TCTACCGGTC AACCTACCAA TCAATCCATG GATGATACAA 1021 GAGAGGATAT TTATGTCAAC TATCCAACCT TCTCTCGCAG ACCAAAGACT AGAGTTTTGC 1081 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1141 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1201 ATATATCTTG TGGAAAGGAC GATGTTGGCT CTCGCACATG CACCCAACGC GTTAAGTCga 1261 caatcaacct ctggattaca aaatttgtga aagatt