Construct: ORF TRCN0000480827
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001706.1_s317c1
- Derived from:
- ccsbBroadEn_08496
- DNA Barcode:
- CGCTCATGACCGGATGACATGGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC25 (55246)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480827
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55246 | CCDC25 | coiled-coil domain containi... | NM_018246.3 | 99.8% | 99% | 621_622insA |
| 2 | human | 55246 | CCDC25 | coiled-coil domain containi... | NM_001304532.2 | 67.2% | 66.5% | 0_1ins204;417_418insA |
| 3 | human | 55246 | CCDC25 | coiled-coil domain containi... | XM_011544570.2 | 67.2% | 66.5% | 0_1ins204;417_418insA |
| 4 | human | 55246 | CCDC25 | coiled-coil domain containi... | XM_011544571.2 | 67.2% | 66.5% | 0_1ins204;417_418insA |
| 5 | human | 55246 | CCDC25 | coiled-coil domain containi... | NM_001304530.2 | 57.2% | 55.9% | (many diffs) |
| 6 | human | 55246 | CCDC25 | coiled-coil domain containi... | NM_001304529.2 | 40.1% | 39.2% | (many diffs) |
| 7 | human | 55246 | CCDC25 | coiled-coil domain containi... | NR_156432.2 | 14.3% | (many diffs) | |
| 8 | human | 55246 | CCDC25 | coiled-coil domain containi... | NR_130761.2 | 13% | (many diffs) | |
| 9 | human | 55246 | CCDC25 | coiled-coil domain containi... | NR_130762.2 | 12.8% | (many diffs) | |
| 10 | mouse | 67179 | Ccdc25 | coiled-coil domain containi... | NM_145944.4 | 91.8% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 693
- ORF length:
- 627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gttctacttc accagcagca gcgttaattc atctgcctac actatttaca 121 tgggaaaaga taaatatgaa aatgaagatc tgatcaagca tggctggcct gaagatatct 181 ggtttcatgt ggacaaactc tcttcggctc atgtatacct tcgattacat aagggagaga 241 atatagaaga catcccaaag gaagtgctga tggactgtgc ccaccttgtg aaggCCAATA 301 GCATTCAAGG CTGCAAGATG AACAACGTTA ATGTGGTATA TACGCCGTGG TCTAACCTGA 361 AGAAAACAGC TGACATGGAT GTGGGGCAGA TAGGCTTTCA CAGGCAGAAG GATGTAAAAA 421 TTGTGACAGT GGAGAAGAAA GTAAATGAGA TCCTGAACCG ATTAGAAAAG ACCAAAGTCG 481 AGCGGTTCCC AGACCTAGCA GCAGAGAAAG AATGCAGAGA TCGTGAAGAG AGGAATGAGA 541 AAAAAGCCCA AATTCAGGAA ATGAAAAAGA GAGAAAAAGA AGAAATGAAG AAGAAGAGGG 601 AAATGGATGA ACTTAGGAGC TATTCATCAC TAATGAAAGT TGAAAATATG TCTTCAAATC 661 AGGATGGCAA TGATTCAGAT GAATTCAATG TACCCAACTT TCTTGTACAA AGTGGTTGAT 721 ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT 781 CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGCT 841 CATGACCGGA TGACATGGGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt 901 gtgaaagatt