Transcript: Human NM_018246.3

Homo sapiens coiled-coil domain containing 25 (CCDC25), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
CCDC25 (55246)
Length:
3627
CDS:
101..727

Additional Resources:

NCBI RefSeq record:
NM_018246.3
NBCI Gene record:
CCDC25 (55246)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_018246.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000247094 TGCAAGATGAACAACGTTAAT pLKO_005 347 CDS 100% 13.200 18.480 N Ccdc25 n/a
2 TRCN0000424038 GTATATACGCCGTGGTCTAAC pLKO_005 371 CDS 100% 10.800 15.120 N CCDC25 n/a
3 TRCN0000143156 CAGGATGGCAATGATTCAGAT pLKO.1 695 CDS 100% 4.950 6.930 N CCDC25 n/a
4 TRCN0000122667 GCTGCAAGATGAACAACGTTA pLKO.1 345 CDS 100% 4.950 6.930 N CCDC25 n/a
5 TRCN0000122416 GAGATCCTGAACCGATTAGAA pLKO.1 482 CDS 100% 5.625 4.500 N CCDC25 n/a
6 TRCN0000143707 GTCTTCAAATCAGGATGGCAA pLKO.1 685 CDS 100% 2.640 2.112 N CCDC25 n/a
7 TRCN0000415953 GTTGAACTTGACAGCATATAA pLKO_005 925 3UTR 100% 15.000 10.500 N CCDC25 n/a
8 TRCN0000415274 CTGTCCCTGATTTCTCGATTA pLKO_005 1169 3UTR 100% 10.800 7.560 N CCDC25 n/a
9 TRCN0000143877 CATTCAAGGCTGCAAGATGAA pLKO.1 337 CDS 100% 4.950 3.465 N CCDC25 n/a
10 TRCN0000144631 GATGGCAATGATTCAGATGAA pLKO.1 698 CDS 100% 4.950 3.465 N CCDC25 n/a
11 TRCN0000143604 GCAGAGAAAGAATGCAGAGAT pLKO.1 536 CDS 100% 4.950 2.970 N CCDC25 n/a
12 TRCN0000144883 GAAATGAAGAAGAAGAGGGAA pLKO.1 617 CDS 100% 2.640 1.584 N CCDC25 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_018246.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08496 pDONR223 100% 99.8% 99% None 621_622insA n/a
2 ccsbBroad304_08496 pLX_304 0% 99.8% 99% V5 (not translated due to frame shift) 621_622insA n/a
3 TRCN0000480827 CGCTCATGACCGGATGACATGGGC pLX_317 63.1% 99.8% 99% V5 (not translated due to prior stop codon) 621_622insA n/a
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