Construct: ORF TRCN0000480830
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000785.1_s317c1
- Derived from:
- ccsbBroadEn_05741
- DNA Barcode:
- TATACTAGCCTCATTATCCTGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TVP23A (780776)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480830
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 780776 | TVP23A | trans-golgi network vesicle... | NM_001079512.4 | 100% | 100% | |
| 2 | human | 780776 | TVP23A | trans-golgi network vesicle... | XM_006720944.3 | 100% | 100% | |
| 3 | human | 780776 | TVP23A | trans-golgi network vesicle... | XM_011522654.3 | 100% | 100% | |
| 4 | human | 780776 | TVP23A | trans-golgi network vesicle... | XM_017023649.2 | 100% | 100% | |
| 5 | human | 780776 | TVP23A | trans-golgi network vesicle... | NM_001318873.2 | 69.9% | 69.9% | 0_1ins192 |
| 6 | human | 780776 | TVP23A | trans-golgi network vesicle... | XM_017023650.2 | 69.9% | 69.9% | 0_1ins192 |
| 7 | human | 780776 | TVP23A | trans-golgi network vesicle... | XM_017023651.2 | 69.9% | 69.9% | 0_1ins192 |
| 8 | human | 780776 | TVP23A | trans-golgi network vesicle... | XR_001751978.1 | 42.5% | 1_409del;1049_1501del | |
| 9 | human | 780776 | TVP23A | trans-golgi network vesicle... | NR_134902.2 | 35.3% | 1_301del;941_1806del | |
| 10 | human | 780776 | TVP23A | trans-golgi network vesicle... | NR_134901.2 | 34% | 1_301del;389_458del;1011_1876del | |
| 11 | human | 780776 | TVP23A | trans-golgi network vesicle... | NR_134903.2 | 33.5% | (many diffs) | |
| 12 | human | 780776 | TVP23A | trans-golgi network vesicle... | XR_001751979.1 | 31.5% | 1_409del;1049_2028del | |
| 13 | human | 780776 | TVP23A | trans-golgi network vesicle... | NR_134904.2 | 29.6% | (many diffs) | |
| 14 | human | 780776 | TVP23A | trans-golgi network vesicle... | NR_134905.2 | 27.3% | 1_301del;388_389ins145;796_1661del | |
| 15 | human | 780776 | TVP23A | trans-golgi network vesicle... | NR_134906.2 | 25.7% | 1_301del;388_389ins145;796_1777del | |
| 16 | mouse | 383103 | Tvp23a | trans-golgi network vesicle... | XM_017317060.1 | 85.7% | 89.2% | (many diffs) |
| 17 | mouse | 383103 | Tvp23a | trans-golgi network vesicle... | NM_001013778.1 | 81.8% | 84.6% | (many diffs) |
| 18 | mouse | 383103 | Tvp23a | trans-golgi network vesicle... | XM_006522315.3 | 79% | 82.2% | (many diffs) |
| 19 | mouse | 383103 | Tvp23a | trans-golgi network vesicle... | XM_006522314.3 | 75.6% | 78.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 708
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagcaggcc ctggtggacg ataccgagga tgtgtccctg gactttggaa 121 acgaggagga gctggccttt aggaaagcca agatcagaca ccccttggcc acctttttcc 181 acctgttttt ccgagtgagt gccatcgtca cctacgtgag ctgcgactgg ttcagcaaga 241 gctttgtggg ctgttttgtc atggtgctgc tcctcctgtc cctggacttc tggtctgtga 301 agaatgtaac cggaagactc ctggtgggcc TTCGATGGTG GAACCAGATA GATGAAGATG 361 GGAAGAGCCA CTGGATCTTT GAAGCCAGGA AGGTCTCTCC GAATAGCATT GCTGCCACAG 421 AAGCTGAAGC ACGAATCTTC TGGCTGGGCC TCATAATCTG CCCCATGATA TGGATTGTGT 481 TTTTTTTTAG CACCTTATTT TCCTTGAAGC TAAAGTGGCT GGCTCTGGTG GTTGCTGGGA 541 TCTCTCTCCA AGCTGCAAAC CTGTATGGCT ACATCCTTTG TAAGATGGGA GGCAACAGTG 601 ACATTGGCAA GGTCACAGCC AGTTTCCTGT CCCAGACAGT GTTCCAGACG GCCTGCCCAG 661 GTGACTTTCA GAAGCCTGGC CTCGAGGGGC TGGAGATTCA CCAGCATTTG CCAACTTTCT 721 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 781 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 841 GTGGAAAGGA CGATATACTA GCCTCATTAT CCTGTATACG CGTTAAGTCg acaatcaacc 901 tctggattac aaaatttgtg aaagatt