Construct: ORF TRCN0000480849
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008822.1_s317c1
- Derived from:
- ccsbBroadEn_00409
- DNA Barcode:
- TTGTGTCTTGCGGCTCGGGATGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP2C18 (1562)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480849
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1562 | CYP2C18 | cytochrome P450 family 2 su... | NM_000772.3 | 100% | 100% | |
| 2 | human | 1562 | CYP2C18 | cytochrome P450 family 2 su... | NM_001128925.2 | 87.9% | 87.9% | 639_640ins177 |
| 3 | human | 1559 | CYP2C9 | cytochrome P450 family 2 su... | NM_000771.4 | 87.7% | 81.8% | (many diffs) |
| 4 | human | 1557 | CYP2C19 | cytochrome P450 family 2 su... | NM_000769.4 | 87.5% | 80.8% | (many diffs) |
| 5 | human | 1558 | CYP2C8 | cytochrome P450 family 2 su... | NM_000770.3 | 84.5% | 77.1% | (many diffs) |
| 6 | human | 1558 | CYP2C8 | cytochrome P450 family 2 su... | NM_001198853.1 | 72.5% | 66.3% | (many diffs) |
| 7 | human | 1558 | CYP2C8 | cytochrome P450 family 2 su... | NM_001198855.1 | 72.5% | 66.3% | (many diffs) |
| 8 | human | 1558 | CYP2C8 | cytochrome P450 family 2 su... | NM_001198854.1 | 66.5% | 60.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1536
- ORF length:
- 1470
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tccagctgtg gctctggtgc tctgtctctc ctgtttgttt ctcctttcac 121 tctggaggca gagctctgga agagggaggc tcccgtctgg ccccactcct ctcccgatta 181 ttggaaatat cctgcagtta gatgttaagg acatgagcaa atccttaacc aatttctcaa 241 aagtctatgg ccctgtgttc actgtgtatt ttggcctgaa gcccattgtg gtgttgcatg 301 gatatgaagc agtgaaggag gccctgattg atcatggaga ggagttttct ggaagaggaa 361 gttttccagt ggctgaaaaa gttaacaaag gacttggaat ccttttcagc aatggaaaga 421 gatggaagga gatccggcgt ttctgcctca tgactctgcg gaattttggg atggggaaga 481 ggagcatcga ggaccgtgtt caagaggaag cccgctgcct tgtggaggag ttgagaaaaa 541 ccaatgcctc accctgtgat cccactttca tcctgggctg tgctccctgc aatgtgatct 601 gctctgttat tttccatgat cgatttgatt ataaagatca gaggtttctt aacttgatgg 661 aaaaattcaa tgaaaacctc aggattctga gctctccatg gatccaggtc tgcaataatt 721 tccctgctct catcgattat ctcccaggaa gtcataataa aatagctgaa aattttgctt 781 acattaaaag ttatgtattg gagagaataa aagaacatca agaatccctg gacatgaaca 841 gtgctcggga ctttattgat tgtttcctga tcaaaatgga acaggaaaag cacaatcaac 901 agtctgaatt tactgttgaa agcttgatag ccactgtaac tgatatgttt ggggctggaa 961 cagagacaac gagcaccact ctgagatatg gactcctgct cctgctgaag tacccagagg 1021 tcacagctaa agtccaggaa gagattgaat gtgtagttgg cagaaaccgg agcccctgta 1081 tgcaggacag gagtcacatg ccctacacag atgctgtggt gcacgagatc cagagataca 1141 ttgaccTCCT CCCCACCAAC CTGCCCCATG CAGTGACCTG TGATGTTAAA TTCAAAAACT 1201 ACCTCATCCC CAAGGGCACG ACCATAATAA CATCCCTGAC TTCTGTGCTG CACAATGACA 1261 AAGAATTCCC CAACCCAGAG ATGTTTGACC CTGGCCACTT TCTGGATAAG AGTGGCAACT 1321 TTAAGAAAAG TGACTACTTC ATGCCTTTCT CAGCAGGAAA ACGGATGTGT ATGGGAGAGG 1381 GCCTGGCCCG CATGGAGCTG TTTTTATTCC TGACCACCAT TTTGCAGAAC TTTAACCTGA 1441 AATCTCAGGT TGACCCAAAG GATATTGACA TCACCCCCAT TGCCAATGCA TTTGGTCGTG 1501 TGCCACCCTT GTACCAGCTC TGCTTCATTC CTGTCTGCCC AACTTTCTTG TACAAAGTGG 1561 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1621 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1681 ATTGTGTCTT GCGGCTCGGG ATGGGACGCG TTAAGTCgac aatcaacctc tggattacaa 1741 aatttgtgaa agatt