Construct: ORF TRCN0000480860
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004867.1_s317c1
- Derived from:
- ccsbBroadEn_11845
- DNA Barcode:
- GTTGGACTCTATGCTTACAGCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMC1B (27127)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480860
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27127 | SMC1B | structural maintenance of c... | NM_001291501.2 | 99.9% | 99.9% | 3148C>A |
| 2 | human | 27127 | SMC1B | structural maintenance of c... | XM_011530145.2 | 94.3% | 93.7% | (many diffs) |
| 3 | human | 27127 | SMC1B | structural maintenance of c... | NM_148674.5 | 93.9% | 93.9% | 3148C>A;3272_3493del |
| 4 | human | 27127 | SMC1B | structural maintenance of c... | XM_011530144.2 | 90.6% | 90.6% | 0_1ins123;3025C>A;3149_3370del |
| 5 | human | 27127 | SMC1B | structural maintenance of c... | XR_244368.4 | 82.2% | (many diffs) | |
| 6 | mouse | 140557 | Smc1b | structural maintenance of c... | NM_080470.1 | 79.5% | 76.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3552
- ORF length:
- 3483
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccacctg gagctgctgc ttgtggaaaa tttcaagtcg tggcggggcc 121 gccaggtcat tggccccttc cggaggttca cctgcatcat cggccccaac ggctctggaa 181 aatctaatgt aatggatgca cttagttttg taatgggaga gaaaatagct aatttaagag 241 tgaaaaatat tcaagaactc attcatggag cacatattgg aaaacctatt tcttcttctg 301 caagtgtaaa aattatatat gtggaggaaa gtggcgaaga gaaaacattt gcaaggatta 361 tccgaggggg atgctcagaa tttcgcttta atgataatct tgtgagtcgt tctgtttaca 421 ttgcagagtt ggaaaagata ggcataatag tcaaagcaca aaattgtttg gtttttcagg 481 gaactgtaga gtcaatttca gtgaagaaac ccaaagaaag gacccagttt tttgaggaaa 541 tcagcacttc aggagagctt ataggagaat atgaagaaaa gaaaagaaag ttacaaaaag 601 ccgaagagga tgcacagttt aactttaata agaaaaaaaa tatagcggca gagcgcagac 661 aagcaaaatt agagaaggaa gaggcagaac gttaccagag tctccttgaa gaactgaaaa 721 tgaacaagat acaactgcag ctttttcaac tataccataa tgagaaaaag attcatctcc 781 tgaacaccaa gttagagcat gtgaataggg atttgagtgt caaaagagag tctttgtctc 841 atcatgaaaa catagttaaa gccaggaaaa aggaacatgg aatgctaact agacaactac 901 aacaaacaga aaaagaatta aaatcggttg aaaccctttt aaatcagaag aggcctcagt 961 acattaaagc caaagaaaac acttctcacc accttaagaa attagatgtg gctaagaaat 1021 caataaagga cagcgaaaaa caatgttcta aacaggaaga tgatataaaa gccctggaga 1081 cagagctggc tgatttagat gctgcatgga gaagttttga aaagcagatt gaggaagaaa 1141 ttttacataa aaagcgagac attgaactgg aagccagtca gctggatcgt tataaagaac 1201 ttaaggaaca agtaagaaag aaagtagcta caatgactca acaactggaa aaactgcagt 1261 gggaacagaa gacagatgaa gaaagactgg catttgaaaa gaggaggcat ggagaagttc 1321 agggaaatct aaaacaaata aaagaacaaa tagaagatca taaaaaacga atagagaagt 1381 tagaggagta tacaaagaca tgcatggatt gcttgaaaga gaaaaaacag caagaggaaa 1441 ccctagtgga tgaaattgaa aaaacaaaat caagaatgtc tgaagttaat gaagaattga 1501 atcttattag aagtgaattg cagaatgctg ggattgatac ccatgaggga aaacgtcagc 1561 aaaagagagc agaggttctg gaacacctta aaagactgta cccagattct gtgtttggaa 1621 gactatttga cctgtgtcat cctattcata agaaatacca gctggctgtt actaaggttt 1681 ttggccggtt catcactgcc attgttgtag cctctgaaaa ggtagcaaaa gattgtattc 1741 gatttctgaa ggaggaaaga gctgaacctg agacattcct cgctctagat taccttgata 1801 tcaagccaat caatgaaaga ctaagggagc ttaaaggctg taaaatggtg attgatgtca 1861 taaagactca gtttcctcag ctgaagaaag tgattcagtt tgtgtgtgga aatggtcttg 1921 tttgtgagac tatggaagaa gcaaggcata ttgcactcag tggacctgaa agacagaaaa 1981 cagtagctct tgatggaaca ttatttttaa aatctggagt gatctctgga gggtcaagtg 2041 acttaaaata caaggctaga tgctgggatg agaaagagtt aaagaatcta agagacagac 2101 gaagccagaa aatccaagag ctaaagggtt taatgaagac actccgcaaa gaaacagatt 2161 tgaaacaaat acagaccctg atacagggaa ctcaaacacg actcaaatat tcacaaaatg 2221 aactagagat gattaagaag aagcaccttg ttgcttttta ccaggaacaa tctcagttac 2281 aaagtgaact actaaatatt gagtctcaat gtattatgtt gagtgaagga atcaaggaac 2341 gacaacgaag aattaaagaa tttcaagaaa agatagataa ggtagaagac gatatcttcc 2401 aacacttctg tgaagaaatt ggcgtggaaa atattcgtga atttgagaac aaacatgtta 2461 aacggcaaca agaaattgat caaaaaagat tagaatttga aaaacaaaaa actcggctta 2521 atgttcaact tgagtatagt cgcagtcacc ttaagaagaa actgaataag atcaacacat 2581 taaaagaaac tatccagaaa ggtagtgaag atattgatca cctaaagaag gctgaagaaa 2641 actgtctgca gacagtgaat gaactcatgg caaagcagca gcaacttaag gacatacgtg 2701 tcactcagaa ctccagtgcc gagaaagttc aaactcaaat tgaagaggaa cggaagaagt 2761 ttctggctgt tgatagggaa gtggggaaat tgcaaaaaga agttgtaagt attcaaactt 2821 ctctggaaca gaaacgatta gagaagcata acttgctgct tgattgcaaa gtgcaagaca 2881 ttgagataat ccttttgtcg gggtcactgg atgacatcat tgaagtggag atgggaactg 2941 aagcagaaag tacccaggca acaattgata tctatgaaaa agaagaagcc tttgaaatag 3001 actacagctc tctaaaagag gatttgaagg ctctacagtc tgatcaagaa atcgaggccc 3061 accttaggct cttattgcag caagtagcat cccaggaaga tatcttactg aaaacagcag 3121 ccccaaacct acgagcactG GAGAACTTAA AGACTGTCAG AGACAAGTTT CAAGAGTCCA 3181 CAGATGCTTT TGAGGCCAGC AGAAAGGAAG CCAGAATGTG TAGGCAAGAG TTCGAGCAAG 3241 TGAAAAAAAG GAGATACGAT CTTTTCACCC AGTGTTTTGA GCATGTCTCA ATCTCAATTG 3301 ATCAAATCTA CAAGAAGCTC TGCAGAAACA ACAGCGCCCA AGTGTCAAGT TACATCAAAG 3361 AGCAAACTCA AGACCAGTTT CAGATGATAG TCATCTCCCT AAAAGAAGAG TTCTATTCCA 3421 GAGCCGACGC GCTGATCGGC ATCTATCCTG AGTACGATGA CTGCATGTTC AGCCGAGTTT 3481 TGACCCTAGA TCTTTCTCAG TATCCAGACA CTGAAGGCCA AGAAAGCAGC AAGAGACACG 3541 GAGAGTCCCG CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 3601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 3661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTT GGACTCTATG CTTACAGCAA 3721 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t