Construct: ORF TRCN0000480866
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002392.1_s317c1
- Derived from:
- ccsbBroadEn_16145
- DNA Barcode:
- ACATAATCAGAGATTATTCCTTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- P4HA3 (283208)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480866
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | NM_182904.5 | 62% | 61.5% | (many diffs) |
| 2 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | NM_001288748.2 | 55.9% | 55.4% | (many diffs) |
| 3 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747836.1 | 52.9% | (many diffs) | |
| 4 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747837.1 | 51.7% | (many diffs) | |
| 5 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747840.1 | 47.9% | (many diffs) | |
| 6 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | NR_110031.2 | 46.7% | (many diffs) | |
| 7 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747838.1 | 45.1% | (many diffs) | |
| 8 | human | 283208 | P4HA3 | prolyl 4-hydroxylase subuni... | XR_001747839.1 | 38.5% | (many diffs) | |
| 9 | mouse | 320452 | P4ha3 | procollagen-proline, 2-oxog... | NM_177161.4 | 54.3% | 54.4% | (many diffs) |
| 10 | mouse | 320452 | P4ha3 | procollagen-proline, 2-oxog... | XM_006507944.3 | 53.4% | 53.5% | (many diffs) |
| 11 | mouse | 320452 | P4ha3 | procollagen-proline, 2-oxog... | XM_011241831.1 | 51.2% | 51.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1077
- ORF length:
- 1008
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggtcctggg gcgcggctgg cggcgctgct ggcggtgctg gcgctcggga 121 caggagaccc agaaagggct gcggctcggg gcgacacgtt ctcggcgctg accagcgtgg 181 cgcgcgccct ggcgcccgag cgccggctgc tggggctgct gaggcggtac ctgcgcgggg 241 aggaggcgcg gctgcgggac ctgactagat tctacgacaa ggtactttct ttgcatgagg 301 attcaacaac ccctgtggct aaccctctgc ttgcatttac tctcatcaaa cgcctgcagt 361 ctgactggag gaatgtggta catagtctgg aggccagtga gaacatccga gctctgaagg 421 atggctatga gaaggtggag caagaccttc cagcctttga ggaccttgag ggagcagcaa 481 gggccctgat gcggctgcag gacgtgtaca tgctcaatgt gaaaggcctg gcccgaggtg 541 tctttcagag agtcactggc tctgccatca ctgacctgta cagccccaaa cggctctttt 601 ctctcacagg ggatgactgc ttccaagttg gcaaggtggc cTATGACATG GGGGATTATT 661 ACCATGCCAT TCCATGGCTG GAGGAGGCTG TCAGTCTCTT CCGAGGATCT TACGGAGAGT 721 GGAAGACAGA GGATGAGGCA AGTCTAGAAG ATGCCTTGGA TCACTTGGCC TTTGCTTATT 781 TCCGGGCAGG AAATGTTTCG TGTGCCCTCA GCCTCTCTCG GGAGTTTCTT CTCTACAGCC 841 CAGATAATAA GAGGATGGCC AGGAATGTCT TGAAATATGA AAGGCTCTTG GCAGAGAGCC 901 CCAACCACGT GGTAGCTGAG GCTGTCATCC AGAGGCCCAA TATACCCCAC CTGCAGACCA 961 GAGACACCTA CGAGGGGCTA TGTCAGACCC TGGGTTCCCA GCCCACTCTC TACCAGATCC 1021 CTAGCCTCTA CTGTTCCTAT GAGACCAATT CCAACGCCTA CCTGCAGCTC CAGCCCTGAA 1081 GACTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC 1141 CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT 1201 TGGCTTTATA TATCTTGTGG AAAGGACGAA CATAATCAGA GATTATTCCT TTTACGCGTT 1261 AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att