Construct: ORF TRCN0000480906
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005288.3_s317c1
- Derived from:
- ccsbBroadEn_14853
- DNA Barcode:
- TAATCTCTACCGGTACTACACCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK11 (6794)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480906
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6794 | STK11 | serine/threonine kinase 11 | NM_000455.5 | 100% | 100% | |
| 2 | human | 6794 | STK11 | serine/threonine kinase 11 | NM_000455.4:c.790_793del | 99.6% | 60.6% | 786_787insTTGT |
| 3 | mouse | 20869 | Stk11 | serine/threonine kinase 11 | NM_011492.4 | 84.2% | 89.4% | (many diffs) |
| 4 | mouse | 20869 | Stk11 | serine/threonine kinase 11 | NM_001301853.1 | 79.1% | 79.7% | (many diffs) |
| 5 | mouse | 20869 | Stk11 | serine/threonine kinase 11 | NM_001301854.1 | 59.9% | 62.7% | (many diffs) |
| 6 | mouse | 20869 | Stk11 | serine/threonine kinase 11 | XM_006513441.3 | 50.9% | 53.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1365
- ORF length:
- 1299
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggtggtggac ccgcagcagc tgggcatgtt cacggagggc gagctgatgt 121 cggtgggtat ggacacgttc atccaccgca tcgactccac cgaggtcatc taccagccgc 181 gccgcaagcg ggccaagctc atcggcaagt acctgatggg ggacctgctg ggggaaggct 241 cttacggcaa ggtgaaggag gtgctggact cggagacgct gtgcaggagg gccgtcaaga 301 tcctcaagaa gaagaagttg cgaaggatcc ccaacgggga ggccaacgtg aagaaggaaa 361 ttcaactact gaggaggtta cggcacaaaa atgtcatcca gctggtggat gtgttataca 421 acgaagagaa gcagaaaatg tatatggtga tggagtactg cgtgtgtggc atgcaggaaa 481 tgctggacag cgtgccggag aagcgtttcc cagtgtgcca ggcccacggg tacttctgtc 541 agctgattga cggcctggag tacctgcata gccagggcat tgtgcacaag gacatcaagc 601 cggggaacct gctgctcacc accggtggca ccctcaaaat ctccgacctg ggcgtggccg 661 aggcactgca cccgttcgcg gcggacgaca cctgccggac cagccagggc tccccggctt 721 tccagccgcc cgagattgcc aacggcctgg acaccttctc cggcttcaag gtggacatct 781 ggtcggctgg ggtcaccctc tacaacatca ccacgggtct gtaccccttc gaaggggaca 841 acatctacaa gttgtttgag aacatcggga aggggagcta cgccatcccg ggcgactgtg 901 gccccccgct ctctgacctg ctgaaaggga tgcttgagta cgaaccggcc aagaggttct 961 ccaTCCGGCA GATCCGGCAG CACAGCTGGT TCCGGAAGAA ACATCCTCCG GCTGAAGCAC 1021 CAGTGCCCAT CCCACCGAGC CCAGACACCA AGGACCGGTG GCGCAGCATG ACTGTGGTGC 1081 CGTACTTGGA GGACCTGCAC GGCGCGGACG AGGACGAGGA CCTCTTCGAC ATCGAGGATG 1141 ACATCATCTA CACTCAGGAC TTCACGGTGC CCGGACAGGT CCCAGAAGAG GAGGCCAGTC 1201 ACAATGGACA GCGCCGGGGC CTCCCCAAGG CCGTGTGTAT GAACGGCACA GAGGCGGCGC 1261 AGCTGAGCAC CAAATCCAGG GCGGAGGGCC GGGCCCCCAA CCCTGCCCGC AAGGCCTGCT 1321 CCGCCAGCAG CAAGATCCGC CGGCTGTCGG CCTGCAAGCA GCAGTGCCCA ACTTTCTTGT 1381 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1441 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1501 GAAAGGACGA TAATCTCTAC CGGTACTACA CCAAACGCGT TAAGTCgaca atcaacctct 1561 ggattacaaa atttgtgaaa gatt