Construct: ORF TRCN0000480916
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001714.3_s317c1
- Derived from:
- ccsbBroadEn_14745
- DNA Barcode:
- TCGCACACGGACTCGTTGGGCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PFKL (5211)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480916
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5211 | PFKL | phosphofructokinase, liver ... | NM_001002021.3 | 99.5% | 99.6% | 1_9delATGAGAAGC;516C>T |
| 2 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_024452085.1 | 99.5% | 99.6% | 1_9delATGAGAAGC;516C>T |
| 3 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_006724011.1 | 96.9% | 96.9% | 1_9delATGAGAAGC;309_377del;585C>T |
| 4 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028368.1 | 96.9% | 96.9% | 1_9delATGAGAAGC;309_377del;585C>T |
| 5 | human | 5211 | PFKL | phosphofructokinase, liver ... | NM_002626.6 | 92.5% | 91.7% | (many diffs) |
| 6 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028369.1 | 91.4% | 91.5% | 0_1ins210;297C>T |
| 7 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_005261137.4 | 90.7% | 90.8% | 0_1ins228;279C>T |
| 8 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028370.1 | 90.7% | 90.8% | 0_1ins228;279C>T |
| 9 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028372.1 | 90.7% | 90.8% | 0_1ins228;279C>T |
| 10 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_005261135.3 | 90% | 89.2% | (many diffs) |
| 11 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_011529603.2 | 66.3% | 64.1% | (many diffs) |
| 12 | mouse | 18641 | Pfkl | phosphofructokinase, liver,... | NM_008826.4 | 81.5% | 85.8% | (many diffs) |
| 13 | mouse | 18641 | Pfkl | phosphofructokinase, liver,... | XM_017313846.1 | 49.2% | 52.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2547
- ORF length:
- 2481
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg taaccagggt agaggtcgag agtcctctcg tgggggtctc catgttcaag 121 ggagctgccg aggcttgagc aggagccccc agcaggaaac tggctttgcc aaggcccccg 181 ctgggacaga ctgtttcttt cactgcagtc ctgggagccg agggcaaggg gacaggaaag 241 aggaagtgac ctcagagcct ggtggcacca gcatcatgtc caggctgggg ggcatgaacg 301 ctgctgtccg ggctgtgacg cgcatgggca tttatgtggg tgccaaagtc ttcctcatct 361 acgagggcta tgagggcctc gtggagggag gtgagaacat caagcaggcc aactggctga 421 gcgtctccaa catcatccag ctgggcggca ctatcattgg cagcgctcgc tgcaaggcct 481 ttaccaccag ggaggggcgc cgggcagcgg cctacaacct ggtccagcac ggcatcacca 541 acctgtgcgt catcggcggg gatggcagcc ttacaggtgc caacatcttc cgcagcgagt 601 ggggcagcct gctggaggag ctggtggcgg aaggtaagat ctcagagact acagcccgga 661 cctactcgca cctgaacatc gcgggcctag tgggctccat cgataacgac ttctgcggca 721 ccgacatgac catcggcacg gactcggccc tccaccgcat catggaggtc atcgatgcca 781 tcaccaccac tgcccagagc caccagagga ccttcgtgct ggaagtgatg ggccggcact 841 gcgggtacct ggcgctggta tctgcactgg cctcaggggc cgactggctg ttcatccccg 901 aggctccacc cgaggacggc tgggagaact tcatgtgtga gaggctgggt gagactcgga 961 gccgtgggtc ccgactgaac atcatcatca tcgctgaggg tgccattgac cgcaacggga 1021 agcccatctc gtccagctac gtgaaggacc tggtggttca gaggctgggc ttcgacaccc 1081 gtgtaactgt gctgggccac gtgcagcggg gagggacgcc ctctgccttc gaccggatcc 1141 tgagcagcaa gatgggcatg gaggcggtga tggcgctgct ggaagccacg cctgacacgc 1201 cggcctgcgt ggtcaccctc tcggggaacc agtcagtgcg gctgcccctc atggagtgcg 1261 tgcagatgac caaggaagtg cagaaagcca tggatgacaa gaggtttgac gaggccaccc 1321 agctccgtgg tgggagcttc gagaacaact ggaacattta caagctcctc gcccaccaga 1381 agccccccaa ggagaagtct aacttctccc tggccatcct gaatgtgggg gccccggcgg 1441 ctggcatgaa tgcggccgtg cgctcggcgg tgcggaccgg catctcccat ggacacacag 1501 tatacgtggt gcacgatggc ttcgaaggcc tagccaaggg tcaggtgcaa gaagtaggct 1561 ggcacgacgt ggccggctgg ttggggcgtg gtggctccat gctggggacc aagaggaccc 1621 tgcccaaggg ccagctggag tccattgtgg agaacatccg catctatggt attcacgccc 1681 tgctggtggt cggtgggttt gaggcctatg aaggggtgct gcagctggtg gaggctcgcg 1741 ggcgctacga ggagctctgc atcgtcatgt gtgtcatccc agccaccatc agcaacaacg 1801 tccctggcac cgacttcagc ctgggctccg acactgctgt aaatgccgcc atggagagct 1861 gtgaccgcat caaacagtct gcctcgggga ccaagcgccg tgtgttcatc gtggagacca 1921 tggggggtta ctgtggctac ctggccaccg tgactggcat tgctgtgggg gccgacgccg 1981 cctacgtctt cgaggaccct ttcaacatcc acgacttaaa ggtcaacgtg gagcacatga 2041 cggagaagat gaagacagac attcagaggg gcctggtgct gcggaacgag aagtgccatg 2101 actacTACAC CACGGAGTTC CTGTACAACC TGTACTCATC AGAGGGCAAG GGCGTCTTCG 2161 ACTGCAGGAC CAATGTCCTG GGCCACCTGC AGCAGGGTGG CGCTCCAACC CCCTTTGACC 2221 GGAACTATGG GACCAAGCTG GGGGTGAAGG CCATGCTGTG GTTGTCGGAG AAGCTGCGCG 2281 AGGTTTACCG CAAGGGACGG GTGTTCGCCA ATGCCCCAGA CTCGGCCTGC GTGATCGGCC 2341 TGAAGAAGAA GGCGGTGGCC TTCAGCCCCG TCACTGAGCT CAAGAAAGAC ACTGATTTCG 2401 AGCACCGCAT GCCACGGGAG CAGTGGTGGC TGAGCCTGCG GCTCATGCTG AAGATGCTGG 2461 CACAATACCG CATCAGTATG GCCGCCTACG TGTCAGGGGA GCTGGAGCAC GTGACCCGCC 2521 GCACCCTGAG CATGGACAAG GGCTTCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 2581 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2641 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCGCACAC 2701 GGACTCGTTG GGCGATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2761 aagatt